Restriction map for
pF1KB5348
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII AccIII AclI AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI MfeI MluI MluNI
MroI MscI MspI MunI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmeI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 1064 GGTACC G^GTACC
AccI 275 GTAGAC GT^[AC][GT]AC
AccII 1102 CGCG CG^CG
AcsI 408 GAATTT [GA]^AATT[CT]
ApaI 895 GGGCCC GGGCC^C
ApoI 408 GAATTT [GA]^AATT[CT]
Asp718I 1064 GGTACC G^GTACC
BanI 1064 GGTACC G^G[CT][GA]CC
BfaI 508 CTAG C^TAG
BglII 490 AGATCT A^GATCT
BpmI 1127 CTGGAG CTGGAG(16/14)
BsaBI 757 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 494 CTGCAC GTGCAG(16/14)
BsrBI 592 CCGCTC CCGCTC(-3/-3)
BstUI 1102 CGCG CG^CG
BstXI 233 CCATCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 490 AGATCT [GA]^GATC[CT]
Cac8I 1120 GCTGGC GC[ACGT]^[ACGT]GC
DraIII 872 CACGTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1101 CCGCGG C^C[GA][CT]GG
FauI 900 CCCGC CCCGC(4/6)
HgaI 181 GACGC GACGC(5/10)
HphI 161 TCACC GGTGA(8/7)
KpnI 1064 GGTACC GGTAC^C
KspI 1101 CCGCGG CCGC^GG
MaeI 508 CTAG C^TAG
MamI 757 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 490 AGATCT [GA]^GATC[CT]
MspA1I 1101 CCGCGG C[AC]G^C[GT]G
MvnI 1102 CGCG CG^CG
NdeI 851 CATATG CA^TATG
SacI 539 GAGCTC GAGCT^C
SacII 1101 CCGCGG CCGC^GG
ScaI 108 AGTACT AGT^ACT
SfaNI 190 GATGC GCATC(5/9)
SpeI 507 ACTAGT A^CTAGT
Tsp509I 409 AATT ^AATT
TspRI 522 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 490 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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AfaI 109 GTAC GT^AC
1065 GTAC
BanII 539 GAGCTC G[GA]GC[CT]^C
895 GGGCCC
BsmAI 200 GAGAC GTCTC(1/5)
971 GAGAC
BsrI 442 CCAGT ACTGG(1/-1)
746 CCAGT
DdeI 185 CTAAG C^T[ACGT]AG
485 CTAAG
EcoNI 313 CCTCCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
367 CCTTCACGAGG
HaeIII 896 GGCC GG^CC
1123 GGCC
HinfI 847 GATTC G^A[ACGT]TC
1081 GATTC
MseI 44 TTAA T^TAA
413 TTAA
PflMI 463 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
904 CCATCTTATGG
RsaI 109 GTAC GT^AC
1065 GTAC
SapI 536 GAAGAGC GCTCTTC(1/4)
27 GCTCTTC
SmlI 981 CTCAAG C^T[CT][GA]AG
1005 CTCAAG
TaqI 582 TCGA T^CGA
969 TCGA
TfiI 847 GATTC G^A[AT]TC
1081 GATTC
Tru9I 44 TTAA T^TAA
413 TTAA
TthHB8I 582 TCGA T^CGA
969 TCGA
Van91I 463 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
904 CCATCTTATGG
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AspHI AvaII BbrPI
BbvI BmyI BsaAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BssKI BssSI BstF5I BstNI Cfr13I
DpnII DraII EarI EcoO109I EcoRII
Fnu4HI FokI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MslI
MvaI MwoI NdeII NlaIII NlaIV
PmaCI PmlI PpuMI Sau3AI Sau96I
ScrFI SfcI TseI Tsp45I