##################################
# NO CUT ENZYMES (  210 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmBI       BsmFI       BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BsrI        BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       HphI        KasI        KpnI        KspI        
MaeIII      MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MspI        MunI        MvaI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScrFI       
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        StyI        
SwaI        Tsp45I      Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AflIII        114  ACGTGT               A^C[GA][CT]GT
AlwI          364  GATCC                GGATC(4/5)
Asp700I       220  GAAGCGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI          382  GGTGCC               G^G[CT][GA]CC
BbvI          328  GCTGC                GCAGC(8/12)
BfaI          106  CTAG                 C^TAG
BsmAI         510  GAGAC                GTCTC(1/5)
BsmI          522  GCATTC               GAATGC(1/-1)
BspHI         416  TCATGA               T^CATGA
BsrDI          20  CATTGC               GCAATG(2/0)
DdeI          236  CTCAG                C^T[ACGT]AG
Fnu4HI        328  GCTGC                GC^[ACGT]GC
HaeIII          3  GGCC                 GG^CC
ItaI          328  GCTGC                GC^[ACGT]GC
MaeI          106  CTAG                 C^TAG
MaeII         114  ACGT                 A^CGT
MseI          230  TTAA                 T^TAA
MwoI          457  GCGAAAGAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII        417  CATG                 CATG^
RcaI          416  TCATGA               T^CATGA
ScaI          102  AGTACT               AGT^ACT
SspI          137  AATATT               AAT^ATT
TaqI          111  TCGA                 T^CGA
Tru9I         230  TTAA                 T^TAA
TseI          328  GCTGC                G^C[AT]GC
TthHB8I       111  TCGA                 T^CGA
XmnI          220  GAAGCGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcsI           85  GAATTT               [GA]^AATT[CT]
              195  AAATTT              
AfaI          103  GTAC                 GT^AC
              263  GTAC                
AluI          243  AGCT                 AG^CT
              543  AGCT                
AlwNI          74  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              164  CAGATGCTG           
ApoI           85  GAATTT               [GA]^AATT[CT]
              195  AAATTT              
Cac8I         244  GCTGGC               GC[ACGT]^[ACGT]GC
              453  GCTGGC              
DpnII         109  GATC                 ^GATC
              364  GATC                
EarI           67  GAAGAG               CTCTTC(1/4)
              505  GAAGAG              
HinfI         292  GAATC                G^A[ACGT]TC
              430  GATTC               
Ksp632I        67  GAAGAG               CTCTTC(1/4)
              505  GAAGAG              
MboI          109  GATC                 ^GATC
              364  GATC                
NdeII         109  GATC                 ^GATC
              364  GATC                
NlaIV         183  GGAACC               GG[ACGT]^[ACGT]CC
              382  GGTGCC              
RsaI          103  GTAC                 GT^AC
              263  GTAC                
Sau3AI        109  GATC                 ^GATC
              364  GATC                
SfaNI         166  GATGC                GCATC(5/9)
              325  GATGC               
TfiI          292  GAATC                G^A[AT]TC
              430  GATTC               
TspRI         288  CAGTG                CA[GC]TG[ACGT][ACGT]^
              396  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

MboII       MnlI        Tsp509I