Restriction map for
pF1KB5523
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# NO CUT ENZYMES ( 60 enzymes) #
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Acc65I AccI AccIII AclI AgeI
AscI AseI AsnI Asp718I AvrII
BanI BbeI BbrPI BlnI BseAI
Bsp1407I BspEI BspLU11I BsrGI BssHII
Bst1107I BstBI BstZ17I CpoI DraI
EcoT22I FseI HpaI KasI KpnI
KspI MluI MroI NarI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I RsrII SacII
SalI SfoI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI XhoI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 4601 GACGTC GACGT^C
AflII 1516 CTTAAG C^TTAAG
AflIII 6141 ACACGT A^C[GA][CT]GT
Alw44I 3939 GTGCAC G^TGCAC
ApaLI 3939 GTGCAC G^TGCAC
AspI 925 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 176 GGATAC GTATCC(6/5)
BfrI 1516 CTTAAG C^TTAAG
BlpI 1855 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1855 GCTGAGC GC^T[ACGT]AGC
BsaBI 124 GATTCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 3056 CGTACG C^GTACG
BsmI 2541 GAATGC GAATGC(1/-1)
BstEII 2955 GGTCACC G^GT[ACGT]ACC
BstPI 2955 GGTCACC G^GT[ACGT]ACC
CelII 1855 GCTGAGC GC^T[ACGT]AGC
DraIII 3598 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 5376 CGGCCG C^GGCCG
EclXI 5376 CGGCCG C^GGCCG
Eco52I 5376 CGGCCG C^GGCCG
EcoO65I 2955 GGTCACC G^GT[ACGT]ACC
EcoRI 6979 GAATTC G^AATTC
EcoRV 2974 GATATC GAT^ATC
HincII 1921 GTCAAC GT[CT]^[GA]AC
HindII 1921 GTCAAC GT[CT]^[GA]AC
MamI 124 GATTCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 509 CAATTG C^AATTG
MunI 509 CAATTG C^AATTG
PflFI 925 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 3030 CGATCG CGAT^CG
SbfI 3927 CCTGCAGG CCTGCA^GG
SexAI 6502 ACCTGGT A^CC[AT]GGT
SfiI 6156 GGCCATCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 3927 CCTGCAGG CCTGCA^GG
Tth111I 925 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 918 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 9 enzymes) #
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ApaI 1697 GGGCCC GGGCC^C
7241 GGGCCC
BsiEI 3030 CGATCG CG[GA][CT]^CG
5376 CGGCCG
BsmBI 7323 CGTCTC CGTCTC(1/5)
2389 GAGACG
BspDI 131 ATCGAT AT^CGAT
4162 ATCGAT
BstXI 1169 CCAGTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2232 CCAAGATGCTGG
ClaI 131 ATCGAT AT^CGAT
4162 ATCGAT
DrdI 5752 GACTTCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7315 GACTACTGCGTC
NheI 1704 GCTAGC G^CTAGC
4098 GCTAGC
ScaI 458 AGTACT AGT^ACT
2681 AGTACT
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# 3 OR MORE CUT ENZYMES ( 153 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI AlwI AlwNI AocI
Aor51HI ApoI Asp700I AspEI AspHI
AvaI AvaII AviII BalI BamHI
BanII BbsI BbvCI BbvI BcgI
BclI BcnI BfaI BglI BglII
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI XmnI