################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AflIII AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglI BglII BlnI BmyI BpuAI BsaAI BsaBI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I ClaI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MspA1I MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RcaI SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspI StuI SwaI Tsp509I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccI 744 GTCTAC GT^[AC][GT]AC AccIII 25 TCCGGA T^CCGGA AcyI 102 GGCGTC G[GA]^CG[CT]C AgeI 689 ACCGGT A^CCGGT AlwNI 574 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG BalI 545 TGGCCA TGG^CCA BanI 289 GGTGCC G^G[CT][GA]CC BclI 776 TGATCA T^GATCA BcnI 155 CCGGG CC^[GC]GG BfaI 389 CTAG C^TAG BpmI 595 CTGGAG CTGGAG(16/14) BsaHI 102 GGCGTC G[GA]^CG[CT]C BsaI 751 GAGACC GGTCTC(1/5) BseAI 25 TCCGGA T^CCGGA BseRI 355 GAGGAG GAGGAG(10/8) BsgI 107 CTGCAC GTGCAG(16/14) BsmAI 751 GAGAC GTCTC(1/5) BsmI 68 GCATTC GAATGC(1/-1) Bsp1407I 422 TGTACA T^GTACA BspEI 25 TCCGGA T^CCGGA BsrGI 422 TGTACA T^GTACA BstAPI 603 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 150 GGTGACC G^GT[ACGT]ACC BstF5I 74 CATCC GGATG(2/0) BstPI 150 GGTGACC G^GT[ACGT]ACC CfoI 432 GCGC GCG^C CpoI 22 CGGTCCG CG^G[AT]CCG DraII 157 GGGACCT [GA]G^G[ACGT]CC[CT] EcoO109I 157 GGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 150 GGTGACC G^GT[ACGT]ACC FbaI 776 TGATCA T^GATCA FokI 74 CATCC GGATG(9/13) HaeII 431 GGCGCT [GA]GCGC^[CT] HgaI 103 GCGTC GACGC(5/10) HhaI 432 GCGC GCG^C Hin1I 102 GGCGTC G[GA]^CG[CT]C HinP1I 432 GCGC G^CGC HincII 268 GTCAAC GT[CT]^[GA]AC HindII 268 GTCAAC GT[CT]^[GA]AC MaeI 389 CTAG C^TAG MboII 438 GAAGA GAAGA(8/7) MluNI 545 TGGCCA TGG^CCA MroI 25 TCCGGA T^CCGGA MscI 545 TGGCCA TGG^CCA MseI 176 TTAA T^TAA MslI 651 CATGCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 428 GCCGGC GCC^GGC NciI 155 CCGGG CC^[GC]GG NgoMIV 428 GCCGGC G^CCGGC PinAI 689 ACCGGT A^CCGGT PleI 266 GAGTC GAGTC(4/5) PpuMI 157 GGGACCT [GA]G^G[AT]CC[CT] RsrII 22 CGGTCCG CG^G[AT]CCG SfaNI 673 GATGC GCATC(5/9) SphI 650 GCATGC GCATG^C SspBI 422 TGTACA T^GTACA TfiI 85 GATTC G^A[AT]TC Tru9I 176 TTAA T^TAA XcmI 771 CCACATGATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### BamHI 96 GGATCC G^GATCC 217 GGATCC BlpI 571 GCTCAGC GC^T[ACGT]AGC 612 GCTAAGC Bpu1102I 571 GCTCAGC GC^T[ACGT]AGC 612 GCTAAGC BsaWI 25 TCCGGA [AT]^CCGG[AT] 689 ACCGGT BsmFI 157 GGGAC GGGAC(10/14) 735 GGGAC BsrFI 428 GCCGGC [GA]^CCGG[CT] 689 ACCGGT BstNI 52 CCAGG CC^[AT]GG 413 CCAGG BstYI 96 GGATCC [GA]^GATC[CT] 217 GGATCC CelII 571 GCTCAGC GC^T[ACGT]AGC 612 GCTAAGC Cfr10I 428 GCCGGC [GA]^CCGG[CT] 689 ACCGGT DsaI 146 CCATGG C^C[GA][CT]GG 461 CCATGG EaeI 336 CGGCCA [CT]^GGCC[GA] 545 TGGCCA EcoRII 52 CCAGG ^CC[AT]GG 413 CCAGG FauI 629 CCCGC CCCGC(4/6) 37 GCGGG HinfI 85 GATTC G^A[ACGT]TC 266 GAGTC MaeIII 151 GTGAC ^GT[ACGT]AC 709 GTCAC MflI 96 GGATCC [GA]^GATC[CT] 217 GGATCC MvaI 52 CCAGG CC^[AT]GG 413 CCAGG NcoI 146 CCATGG C^CATGG 461 CCATGG PshAI 159 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 737 GACCATTGTC SfcI 381 CTATAG C^T[GA][CT]AG 746 CTACAG TaqI 182 TCGA T^CGA 436 TCGA Tsp45I 151 GTGAC ^GT[GC]AC 709 GTCAC TthHB8I 182 TCGA T^CGA 436 TCGA XhoII 96 GGATCC [GA]^GATC[CT] 217 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaII BbvI BsaJI BsiYI BslI BsrI BssKI BstUI Cac8I Cfr13I DdeI DpnII EcoT14I Fnu4HI HaeIII HapII HpaII HphI ItaI MboI MnlI MspI MvnI MwoI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI StyI TseI TspRI