Restriction map for
pF1KB5566
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BmyI BpuAI BsaAI BsaBI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1286I
BspDI BspHI BspLU11I BspMI BsrBI
BsrDI BssHII BssSI Bst1107I BstBI
BstXI BstZ17I Bsu36I ClaI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MamI MfeI MluI
MspA1I MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
PvuII RcaI SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tsp509I Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 744 GTCTAC GT^[AC][GT]AC
AccIII 25 TCCGGA T^CCGGA
AcyI 102 GGCGTC G[GA]^CG[CT]C
AgeI 689 ACCGGT A^CCGGT
AlwNI 574 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 545 TGGCCA TGG^CCA
BanI 289 GGTGCC G^G[CT][GA]CC
BclI 776 TGATCA T^GATCA
BcnI 155 CCGGG CC^[GC]GG
BfaI 389 CTAG C^TAG
BpmI 595 CTGGAG CTGGAG(16/14)
BsaHI 102 GGCGTC G[GA]^CG[CT]C
BsaI 751 GAGACC GGTCTC(1/5)
BseAI 25 TCCGGA T^CCGGA
BseRI 355 GAGGAG GAGGAG(10/8)
BsgI 107 CTGCAC GTGCAG(16/14)
BsmAI 751 GAGAC GTCTC(1/5)
BsmI 68 GCATTC GAATGC(1/-1)
Bsp1407I 422 TGTACA T^GTACA
BspEI 25 TCCGGA T^CCGGA
BsrGI 422 TGTACA T^GTACA
BstAPI 603 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 150 GGTGACC G^GT[ACGT]ACC
BstF5I 74 CATCC GGATG(2/0)
BstPI 150 GGTGACC G^GT[ACGT]ACC
CfoI 432 GCGC GCG^C
CpoI 22 CGGTCCG CG^G[AT]CCG
DraII 157 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 157 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 150 GGTGACC G^GT[ACGT]ACC
FbaI 776 TGATCA T^GATCA
FokI 74 CATCC GGATG(9/13)
HaeII 431 GGCGCT [GA]GCGC^[CT]
HgaI 103 GCGTC GACGC(5/10)
HhaI 432 GCGC GCG^C
Hin1I 102 GGCGTC G[GA]^CG[CT]C
HinP1I 432 GCGC G^CGC
HincII 268 GTCAAC GT[CT]^[GA]AC
HindII 268 GTCAAC GT[CT]^[GA]AC
MaeI 389 CTAG C^TAG
MboII 438 GAAGA GAAGA(8/7)
MluNI 545 TGGCCA TGG^CCA
MroI 25 TCCGGA T^CCGGA
MscI 545 TGGCCA TGG^CCA
MseI 176 TTAA T^TAA
MslI 651 CATGCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 428 GCCGGC GCC^GGC
NciI 155 CCGGG CC^[GC]GG
NgoMIV 428 GCCGGC G^CCGGC
PinAI 689 ACCGGT A^CCGGT
PleI 266 GAGTC GAGTC(4/5)
PpuMI 157 GGGACCT [GA]G^G[AT]CC[CT]
RsrII 22 CGGTCCG CG^G[AT]CCG
SfaNI 673 GATGC GCATC(5/9)
SphI 650 GCATGC GCATG^C
SspBI 422 TGTACA T^GTACA
TfiI 85 GATTC G^A[AT]TC
Tru9I 176 TTAA T^TAA
XcmI 771 CCACATGATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BamHI 96 GGATCC G^GATCC
217 GGATCC
BlpI 571 GCTCAGC GC^T[ACGT]AGC
612 GCTAAGC
Bpu1102I 571 GCTCAGC GC^T[ACGT]AGC
612 GCTAAGC
BsaWI 25 TCCGGA [AT]^CCGG[AT]
689 ACCGGT
BsmFI 157 GGGAC GGGAC(10/14)
735 GGGAC
BsrFI 428 GCCGGC [GA]^CCGG[CT]
689 ACCGGT
BstNI 52 CCAGG CC^[AT]GG
413 CCAGG
BstYI 96 GGATCC [GA]^GATC[CT]
217 GGATCC
CelII 571 GCTCAGC GC^T[ACGT]AGC
612 GCTAAGC
Cfr10I 428 GCCGGC [GA]^CCGG[CT]
689 ACCGGT
DsaI 146 CCATGG C^C[GA][CT]GG
461 CCATGG
EaeI 336 CGGCCA [CT]^GGCC[GA]
545 TGGCCA
EcoRII 52 CCAGG ^CC[AT]GG
413 CCAGG
FauI 629 CCCGC CCCGC(4/6)
37 GCGGG
HinfI 85 GATTC G^A[ACGT]TC
266 GAGTC
MaeIII 151 GTGAC ^GT[ACGT]AC
709 GTCAC
MflI 96 GGATCC [GA]^GATC[CT]
217 GGATCC
MvaI 52 CCAGG CC^[AT]GG
413 CCAGG
NcoI 146 CCATGG C^CATGG
461 CCATGG
PshAI 159 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
737 GACCATTGTC
SfcI 381 CTATAG C^T[GA][CT]AG
746 CTACAG
TaqI 182 TCGA T^CGA
436 TCGA
Tsp45I 151 GTGAC ^GT[GC]AC
709 GTCAC
TthHB8I 182 TCGA T^CGA
436 TCGA
XhoII 96 GGATCC [GA]^GATC[CT]
217 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BbvI BsaJI BsiYI BslI
BsrI BssKI BstUI Cac8I Cfr13I
DdeI DpnII EcoT14I Fnu4HI HaeIII
HapII HpaII HphI ItaI MboI
MnlI MspI MvnI MwoI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI StyI TseI TspRI