Restriction map for
pF1KB5597
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AspI AviII AvrII BalI BamHI
BbrPI BclI BfrI BglII BlnI
BsaAI BsaBI BsiWI BsmBI BsmI
Bsp1407I BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I CpoI
DraI DraIII DrdI DsaI Eam1105I
Eco47III Eco81I EcoRI EcoT22I FbaI
FseI FspI HgaI HindIII HpaI
KspI MaeII MamI MfeI MluI
MluNI MscI MseI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SapI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI Tru9I Tth111I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 1064 GGTACC G^GTACC
AccI 1223 GTCGAC GT^[AC][GT]AC
AccIII 1014 TCCGGA T^CCGGA
AcsI 481 AAATTC [GA]^AATT[CT]
ApoI 481 AAATTC [GA]^AATT[CT]
Asp700I 162 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1064 GGTACC G^GTACC
AvaI 212 CCCGGG C^[CT]CG[GA]G
BanII 1008 GAGCTC G[GA]GC[CT]^C
BbsI 1321 GTCTTC GAAGAC(2/6)
BcgI 743 CGAAGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 289 GTATCC GTATCC(6/5)
BlpI 648 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 648 GCTGAGC GC^T[ACGT]AGC
BpuAI 1321 GTCTTC GAAGAC(2/6)
BsaI 685 GAGACC GGTCTC(1/5)
BsaWI 1014 TCCGGA [AT]^CCGG[AT]
BseAI 1014 TCCGGA T^CCGGA
BsiEI 1249 CGGCCG CG[GA][CT]^CG
BsmFI 824 GGGAC GGGAC(10/14)
BsoBI 212 CCCGGG C^[CT]CG[GA]G
BspDI 1240 ATCGAT AT^CGAT
BspEI 1014 TCCGGA T^CCGGA
BspHI 770 TCATGA T^CATGA
BsrBI 60 CCGCTC CCGCTC(-3/-3)
BstAPI 807 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 381 GGTGACC G^GT[ACGT]ACC
BstPI 381 GGTGACC G^GT[ACGT]ACC
BstYI 390 GGATCT [GA]^GATC[CT]
CelII 648 GCTGAGC GC^T[ACGT]AGC
ClaI 1240 ATCGAT AT^CGAT
EaeI 1249 CGGCCG [CT]^GGCC[GA]
EagI 1249 CGGCCG C^GGCCG
EarI 360 GAAGAG CTCTTC(1/4)
EclXI 1249 CGGCCG C^GGCCG
Eco52I 1249 CGGCCG C^GGCCG
EcoO65I 381 GGTGACC G^GT[ACGT]ACC
EcoRV 1158 GATATC GAT^ATC
FauI 1047 GCGGG CCCGC(4/6)
HincII 1223 GTCGAC GT[CT]^[GA]AC
HindII 1223 GTCGAC GT[CT]^[GA]AC
KpnI 1064 GGTACC GGTAC^C
Ksp632I 360 GAAGAG CTCTTC(1/4)
MflI 390 GGATCT [GA]^GATC[CT]
MroI 1014 TCCGGA T^CCGGA
NspI 884 ACATGC [GA]CATG^[CT]
PshAI 459 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 770 TCATGA T^CATGA
SacI 1008 GAGCTC GAGCT^C
SalI 1223 GTCGAC G^TCGAC
SbfI 750 CCTGCAGG CCTGCA^GG
SmaI 212 CCCGGG CCC^GGG
SmlI 877 CTCAAG C^T[CT][GA]AG
Sse8387I 750 CCTGCAGG CCTGCA^GG
StuI 110 AGGCCT AGG^CCT
Tsp509I 482 AATT ^AATT
XhoII 390 GGATCT [GA]^GATC[CT]
XmaCI 212 CCCGGG C^CCGGG
XmaI 212 CCCGGG C^CCGGG
XmnI 162 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 741 CGCG CG^CG
1079 CGCG
AcyI 929 GGCGCC G[GA]^CG[CT]C
1274 GGCGCC
AspHI 1008 GAGCTC G[AT]GC[AT]^C
1255 GTGCTC
BbeI 929 GGCGCC GGCGC^C
1274 GGCGCC
BfaI 25 CTAG C^TAG
985 CTAG
BglI 562 GCCTGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
748 GCCCTGCAGGC
BsaHI 929 GGCGCC G[GA]^CG[CT]C
1274 GGCGCC
BsiHKAI 1008 GAGCTC G[AT]GC[AT]^C
1255 GTGCTC
BsmAI 685 GAGAC GTCTC(1/5)
1134 GTCTC
BsrDI 1209 CATTGC GCAATG(2/0)
1344 CATTGC
BsrFI 926 ACCGGC [GA]^CCGG[CT]
1251 GCCGGT
BstUI 741 CGCG CG^CG
1079 CGCG
BstXI 227 CCAGTGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
386 CCAAGGATCTGG
Cfr10I 926 ACCGGC [GA]^CCGG[CT]
1251 GCCGGT
EcoNI 501 CCTTCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
786 CCTGCTGGAGG
Hin1I 929 GGCGCC G[GA]^CG[CT]C
1274 GGCGCC
KasI 929 GGCGCC G^GCGCC
1274 GGCGCC
MaeI 25 CTAG C^TAG
985 CTAG
MaeIII 78 GTCAC ^GT[ACGT]AC
382 GTGAC
MvnI 741 CGCG CG^CG
1079 CGCG
NarI 929 GGCGCC GG^CGCC
1274 GGCGCC
PleI 341 GACTC GAGTC(4/5)
76 GAGTC
PstI 410 CTGCAG CTGCA^G
751 CTGCAG
SfcI 410 CTGCAG C^T[GA][CT]AG
751 CTGCAG
SfiI 561 GGCCTGTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
747 GGCCCTGCAGGCC
SfoI 929 GGCGCC GGC^GCC
1274 GGCGCC
Tsp45I 78 GTCAC ^GT[GC]AC
382 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BbvCI BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI TseI TspRI TthHB8I Van91I