Restriction map for
pF1KB7031
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BsrBI BsrGI
BssHII Bst1107I BstBI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FseI FspI HaeII
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MseI MunI MvnI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 136 GTCTAC GT^[AC][GT]AC
AflIII 365 ACATGT A^C[GA][CT]GT
AlwI 119 GGATC GGATC(4/5)
Asp700I 768 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 157 CTCGGG C^[CT]CG[GA]G
BanI 818 GGTGCC G^G[CT][GA]CC
BanII 847 GAGCTC G[GA]GC[CT]^C
BclI 182 TGATCA T^GATCA
BcnI 531 CCGGG CC^[GC]GG
BfaI 844 CTAG C^TAG
BsaI 1105 GAGACC GGTCTC(1/5)
BseRI 424 CTCCTC GAGGAG(10/8)
BsmAI 1105 GAGAC GTCTC(1/5)
BsoBI 157 CTCGGG C^[CT]CG[GA]G
BspLU11I 365 ACATGT A^CATGT
BspMI 545 ACCTGC ACCTGC(4/8)
BssSI 603 CTCGTG CACGAG(-5/-1)
BstAPI 143 GCATCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 111 GGTGACC G^GT[ACGT]ACC
BstPI 111 GGTGACC G^GT[ACGT]ACC
BstYI 119 GGATCT [GA]^GATC[CT]
CfoI 744 GCGC GCG^C
DdeI 1012 CTAAG C^T[ACGT]AG
DraII 491 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 649 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 299 CCATGG C^C[GA][CT]GG
EcoO109I 491 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 111 GGTGACC G^GT[ACGT]ACC
FauI 668 CCCGC CCCGC(4/6)
FbaI 182 TGATCA T^GATCA
HgaI 377 GCGTC GACGC(5/10)
HhaI 744 GCGC GCG^C
HinP1I 744 GCGC G^CGC
HindIII 1053 AAGCTT A^AGCTT
MaeI 844 CTAG C^TAG
MaeII 266 ACGT A^CGT
MflI 119 GGATCT [GA]^GATC[CT]
NaeI 644 GCCGGC GCC^GGC
NciI 531 CCGGG CC^[GC]GG
NcoI 299 CCATGG C^CATGG
NgoMIV 644 GCCGGC G^CCGGC
PleI 67 GACTC GAGTC(4/5)
PpuMI 491 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 739 CTGCAG CTGCA^G
PvuII 924 CAGCTG CAG^CTG
SacI 847 GAGCTC GAGCT^C
SmlI 507 CTTGAG C^T[CT][GA]AG
SphI 1112 GCATGC GCATG^C
TfiI 162 GATTC G^A[AT]TC
XhoII 119 GGATCT [GA]^GATC[CT]
XmnI 768 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspHI 421 GTGCTC G[AT]GC[AT]^C
847 GAGCTC
BalI 755 TGGCCA TGG^CCA
899 TGGCCA
BsiHKAI 421 GTGCTC G[AT]GC[AT]^C
847 GAGCTC
BsrFI 644 GCCGGC [GA]^CCGG[CT]
1108 ACCGGC
BsrI 311 ACTGG ACTGG(1/-1)
694 CCAGT
BstF5I 357 CATCC GGATG(2/0)
12 GGATG
Cfr10I 644 GCCGGC [GA]^CCGG[CT]
1108 ACCGGC
EaeI 755 TGGCCA [CT]^GGCC[GA]
899 TGGCCA
EarI 988 CTCTTC CTCTTC(1/4)
1032 CTCTTC
EcoT14I 98 CCTTGG C^C[AT][AT]GG
299 CCATGG
FokI 357 CATCC GGATG(9/13)
12 GGATG
HinfI 67 GACTC G^A[ACGT]TC
162 GATTC
Ksp632I 988 CTCTTC CTCTTC(1/4)
1032 CTCTTC
MluNI 755 TGGCCA TGG^CCA
899 TGGCCA
MscI 755 TGGCCA TGG^CCA
899 TGGCCA
MspA1I 408 CCGCTG C[AC]G^C[GT]G
924 CAGCTG
NspI 365 ACATGT [GA]CATG^[CT]
1112 GCATGC
SfaNI 143 GCATC GCATC(5/9)
413 GCATC
SfcI 138 CTACAG C^T[GA][CT]AG
739 CTGCAG
StyI 98 CCTTGG C^C[AT][AT]GG
299 CCATGG
XcmI 71 CCATTGTGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
116 CCAGGATCTTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AvaII BbsI BbvI
BmyI BpuAI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrDI BssKI BstNI
Cac8I Cfr13I DpnII EcoRII Fnu4HI
HaeIII HapII HpaII HphI ItaI
MaeIII MboI MboII MnlI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TseI
Tsp45I TspRI