Restriction map for pF1KB7039
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# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AgeI        AocI        
Aor51HI     ApaI        ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AvrII       
BamHI       BanI        BbeI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BsaBI       BsaHI       BseAI       
BsiWI       BsmI        BspDI       BspEI       BspLU11I    
BsrBI       BsrDI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      ClaI        DraI        DrdI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       FbaI        FseI        
Hin1I       HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MroI        MseI        MunI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PstI        PvuII       
SacII       SalI        SbfI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     XbaI        XcmI        
XhoI        XmnI        


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# ONE CUT ENZYMES (   47 enzymes) #
###################################

AflIII       1202  ACGTGT               A^C[GA][CT]GT
AhdI         1113  GACCTCCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        222  GTGCAC               G^TGCAC
AlwI          876  GATCC                GGATC(4/5)
ApaLI         222  GTGCAC               G^TGCAC
AspEI        1113  GACCTCCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          760  CCCGGG               C^[CT]CG[GA]G
AviII         644  TGCGCA               TGC^GCA
BanII         241  GAGCTC               G[GA]GC[CT]^C
BbrPI        1201  CACGTG               CAC^GTG
BbsI          454  GAAGAC               GAAGAC(2/6)
BpuAI         454  GAAGAC               GAAGAC(2/6)
BsaWI         192  ACCGGA               [AT]^CCGG[AT]
BsgI         1036  CTGCAC               GTGCAG(16/14)
BsiEI         540  CGATCG               CG[GA][CT]^CG
BsmBI        1354  GAGACG               CGTCTC(1/5)
BsmFI         105  GTCCC                GGGAC(10/14)
BsoBI         760  CCCGGG               C^[CT]CG[GA]G
BspHI        1127  TCATGA               T^CATGA
BsrFI         916  GCCGGC               [GA]^CCGG[CT]
BstYI         875  AGATCC               [GA]^GATC[CT]
Cfr10I        916  GCCGGC               [GA]^CCGG[CT]
CpoI         1186  CGGTCCG              CG^G[AT]CCG
Eam1105I     1113  GACCTCCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I       799  ATGCAT               ATGCA^T
FspI          644  TGCGCA               TGC^GCA
HaeII         350  AGCGCC               [GA]GCGC^[CT]
HincII        532  GTCAAC               GT[CT]^[GA]AC
HindII        532  GTCAAC               GT[CT]^[GA]AC
HinfI         828  GACTC                G^A[ACGT]TC
MflI          875  AGATCC               [GA]^GATC[CT]
NaeI          916  GCCGGC               GCC^GGC
NgoMIV        916  GCCGGC               G^CCGGC
NsiI          799  ATGCAT               ATGCA^T
PleI          828  GACTC                GAGTC(4/5)
PmaCI        1201  CACGTG               CAC^GTG
PmlI         1201  CACGTG               CAC^GTG
PvuI          540  CGATCG               CGAT^CG
RcaI         1127  TCATGA               T^CATGA
RsrII        1186  CGGTCCG              CG^G[AT]CCG
SacI          241  GAGCTC               GAGCT^C
ScaI         1304  AGTACT               AGT^ACT
SmaI          760  CCCGGG               CCC^GGG
SmlI          397  CTCAAG               C^T[CT][GA]AG
XhoII         875  AGATCC               [GA]^GATC[CT]
XmaCI         760  CCCGGG               C^CCGGG
XmaI          760  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AlwNI         334  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              937  CAGGTCCTG           
BalI          278  TGGCCA               TGG^CCA
              692  TGGCCA              
BlpI           87  GCTGAGC              GC^T[ACGT]AGC
              243  GCTCAGC             
Bpu1102I       87  GCTGAGC              GC^T[ACGT]AGC
              243  GCTCAGC             
BsaAI         898  TACGTG               [CT]AC^GT[GA]
             1201  CACGTG              
Bsp1407I      485  TGTACA               T^GTACA
             1002  TGTACA              
BsrGI         485  TGTACA               T^GTACA
             1002  TGTACA              
BsrI          528  ACTGG                ACTGG(1/-1)
             1042  CCAGT               
CelII          87  GCTGAGC              GC^T[ACGT]AGC
              243  GCTCAGC             
DsaI          236  CCACGG               C^C[GA][CT]GG
              908  CCGTGG              
EaeI          278  TGGCCA               [CT]^GGCC[GA]
              692  TGGCCA              
EcoNI         327  CCTGAACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1140  CCTCATGCAGG         
EcoT14I       287  CCAAGG               C^C[AT][AT]GG
              626  CCAAGG              
MluNI         278  TGGCCA               TGG^CCA
              692  TGGCCA              
MscI          278  TGGCCA               TGG^CCA
              692  TGGCCA              
SapI          765  GAAGAGC              GCTCTTC(1/4)
              381  GCTCTTC             
SspBI         485  TGTACA               T^GTACA
             1002  TGTACA              
StyI          287  CCAAGG               C^C[AT][AT]GG
              626  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AspHI       
AvaII       BbvI        BcnI        BmyI        BpmI        
BsaI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BspMI       BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DraIII      
EarI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MnlI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PpuMI       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SphI        StuI        
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
Van91I      
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