################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvrII BamHI BanI BbeI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BsaBI BsaHI BseAI BsiWI BsmI BspDI BspEI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI DraI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FbaI FseI Hin1I HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MseI MunI NarI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuII SacII SalI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AflIII 1202 ACGTGT A^C[GA][CT]GT AhdI 1113 GACCTCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 222 GTGCAC G^TGCAC AlwI 876 GATCC GGATC(4/5) ApaLI 222 GTGCAC G^TGCAC AspEI 1113 GACCTCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 760 CCCGGG C^[CT]CG[GA]G AviII 644 TGCGCA TGC^GCA BanII 241 GAGCTC G[GA]GC[CT]^C BbrPI 1201 CACGTG CAC^GTG BbsI 454 GAAGAC GAAGAC(2/6) BpuAI 454 GAAGAC GAAGAC(2/6) BsaWI 192 ACCGGA [AT]^CCGG[AT] BsgI 1036 CTGCAC GTGCAG(16/14) BsiEI 540 CGATCG CG[GA][CT]^CG BsmBI 1354 GAGACG CGTCTC(1/5) BsmFI 105 GTCCC GGGAC(10/14) BsoBI 760 CCCGGG C^[CT]CG[GA]G BspHI 1127 TCATGA T^CATGA BsrFI 916 GCCGGC [GA]^CCGG[CT] BstYI 875 AGATCC [GA]^GATC[CT] Cfr10I 916 GCCGGC [GA]^CCGG[CT] CpoI 1186 CGGTCCG CG^G[AT]CCG Eam1105I 1113 GACCTCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 799 ATGCAT ATGCA^T FspI 644 TGCGCA TGC^GCA HaeII 350 AGCGCC [GA]GCGC^[CT] HincII 532 GTCAAC GT[CT]^[GA]AC HindII 532 GTCAAC GT[CT]^[GA]AC HinfI 828 GACTC G^A[ACGT]TC MflI 875 AGATCC [GA]^GATC[CT] NaeI 916 GCCGGC GCC^GGC NgoMIV 916 GCCGGC G^CCGGC NsiI 799 ATGCAT ATGCA^T PleI 828 GACTC GAGTC(4/5) PmaCI 1201 CACGTG CAC^GTG PmlI 1201 CACGTG CAC^GTG PvuI 540 CGATCG CGAT^CG RcaI 1127 TCATGA T^CATGA RsrII 1186 CGGTCCG CG^G[AT]CCG SacI 241 GAGCTC GAGCT^C ScaI 1304 AGTACT AGT^ACT SmaI 760 CCCGGG CCC^GGG SmlI 397 CTCAAG C^T[CT][GA]AG XhoII 875 AGATCC [GA]^GATC[CT] XmaCI 760 CCCGGG C^CCGGG XmaI 760 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwNI 334 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG 937 CAGGTCCTG BalI 278 TGGCCA TGG^CCA 692 TGGCCA BlpI 87 GCTGAGC GC^T[ACGT]AGC 243 GCTCAGC Bpu1102I 87 GCTGAGC GC^T[ACGT]AGC 243 GCTCAGC BsaAI 898 TACGTG [CT]AC^GT[GA] 1201 CACGTG Bsp1407I 485 TGTACA T^GTACA 1002 TGTACA BsrGI 485 TGTACA T^GTACA 1002 TGTACA BsrI 528 ACTGG ACTGG(1/-1) 1042 CCAGT CelII 87 GCTGAGC GC^T[ACGT]AGC 243 GCTCAGC DsaI 236 CCACGG C^C[GA][CT]GG 908 CCGTGG EaeI 278 TGGCCA [CT]^GGCC[GA] 692 TGGCCA EcoNI 327 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1140 CCTCATGCAGG EcoT14I 287 CCAAGG C^C[AT][AT]GG 626 CCAAGG MluNI 278 TGGCCA TGG^CCA 692 TGGCCA MscI 278 TGGCCA TGG^CCA 692 TGGCCA SapI 765 GAAGAGC GCTCTTC(1/4) 381 GCTCTTC SspBI 485 TGTACA T^GTACA 1002 TGTACA StyI 287 CCAAGG C^C[AT][AT]GG 626 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AccII AciI AfaI AluI AspHI AvaII BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SphI StuI TaqI TseI Tsp45I TspRI TthHB8I Van91I