Restriction map for
pF1KB7057
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# NO CUT ENZYMES ( 112 enzymes) #
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Acc65I AccI AclI AcsI AflII
AgeI AhdI Alw44I AlwI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BalI
BamHI BbrPI BciVI BclI BfrI
BglII BlnI BsaAI BsaBI BsaI
BsiWI BsmI Bsp1407I BspDI BspLU11I
BsrGI BssHII Bst1107I BstBI BstXI
BstYI BstZ17I ClaI CpoI DpnII
DraI DraIII DrdI Eam1105I Eco47III
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI MamI MboI MfeI MflI
MluI MluNI MscI MseI MunI
NcoI NdeI NdeII NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SalI Sau3AI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI StyI SwaI TfiI
Tru9I Tsp45I Tsp509I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 1462 GACGTC GACGT^C
AspI 208 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 401 TGCGCA TGC^GCA
BbeI 778 GGCGCC GGCGC^C
BbsI 172 GTCTTC GAAGAC(2/6)
BcgI 752 GCAACCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 172 GTCTTC GAAGAC(2/6)
BsiEI 125 CGGCCG CG[GA][CT]^CG
BsmBI 1464 CGTCTC CGTCTC(1/5)
BspHI 1097 TCATGA T^CATGA
BsrBI 388 GAGCGG CCGCTC(-3/-3)
BsrDI 1487 CATTGC GCAATG(2/0)
BssSI 1264 CTCGTG CACGAG(-5/-1)
BstAPI 1164 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1603 GGTAACC G^GT[ACGT]ACC
BstPI 1603 GGTAACC G^GT[ACGT]ACC
EagI 125 CGGCCG C^GGCCG
EclXI 125 CGGCCG C^GGCCG
Eco52I 125 CGGCCG C^GGCCG
EcoO65I 1603 GGTAACC G^GT[ACGT]ACC
FspI 401 TGCGCA TGC^GCA
KasI 778 GGCGCC G^GCGCC
KspI 570 CCGCGG CCGC^GG
MaeIII 1604 GTAAC ^GT[ACGT]AC
MslI 1083 CACCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1419 GCCGGC GCC^GGC
NarI 778 GGCGCC GG^CGCC
NgoMIV 1419 GCCGGC G^CCGGC
NlaIII 1098 CATG CATG^
PflFI 208 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1362 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1097 TCATGA T^CATGA
SacII 570 CCGCGG CCGC^GG
SbfI 312 CCTGCAGG CCTGCA^GG
SfoI 778 GGCGCC GGC^GCC
Sse8387I 312 CCTGCAGG CCTGCA^GG
StuI 18 AGGCCT AGG^CCT
Tth111I 208 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1362 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1493 CCAACAGCCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccIII 1121 TCCGGA T^CCGGA
1131 TCCGGA
AfaI 1383 GTAC GT^AC
1725 GTAC
AflIII 707 ACGTGT A^C[GA][CT]GT
851 ACACGT
AocI 6 CCTGAGG CC^T[ACGT]AGG
1536 CCTCAGG
BfaI 361 CTAG C^TAG
1033 CTAG
BlpI 165 GCTCAGC GC^T[ACGT]AGC
988 GCTGAGC
Bpu1102I 165 GCTCAGC GC^T[ACGT]AGC
988 GCTGAGC
BsaWI 1121 TCCGGA [AT]^CCGG[AT]
1131 TCCGGA
BseAI 1121 TCCGGA T^CCGGA
1131 TCCGGA
BseRI 949 GAGGAG GAGGAG(10/8)
1276 GAGGAG
BsmAI 1118 GTCTC GTCTC(1/5)
1465 GTCTC
BspEI 1121 TCCGGA T^CCGGA
1131 TCCGGA
BsrFI 1219 ACCGGC [GA]^CCGG[CT]
1419 GCCGGC
Bsu36I 6 CCTGAGG CC^T[ACGT]AGG
1536 CCTCAGG
CelII 165 GCTCAGC GC^T[ACGT]AGC
988 GCTGAGC
Cfr10I 1219 ACCGGC [GA]^CCGG[CT]
1419 GCCGGC
DsaI 570 CCGCGG C^C[GA][CT]GG
767 CCGTGG
Eco81I 6 CCTGAGG CC^T[ACGT]AGG
1536 CCTCAGG
HinfI 212 GAGTC G^A[ACGT]TC
1241 GACTC
MaeI 361 CTAG C^TAG
1033 CTAG
MroI 1121 TCCGGA T^CCGGA
1131 TCCGGA
PleI 1241 GACTC GAGTC(4/5)
212 GAGTC
RsaI 1383 GTAC GT^AC
1725 GTAC
SapI 492 GCTCTTC GCTCTTC(1/4)
636 GCTCTTC
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
ApaI AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BsgI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DraII EaeI EarI EcoNI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
Ksp632I MaeII MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIV PpuMI PstI PvuII SacI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI TaqI TseI TspRI TthHB8I
XmaCI XmaI