Restriction map for
pF1KB7062
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BclI
BfaI BglI BlnI BsaBI BsaI
BsaWI BseAI BseRI BsgI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BsrBI BsrDI BssHII BssSI BstBI
BstEII BstPI BstUI BstXI Bsu36I
ClaI CpoI DraI DraIII Eam1105I
Eco47III Eco81I EcoO65I EcoRI FbaI
FseI FspI HincII HindII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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ApaI 13 GGGCCC GGGCC^C
AspHI 154 GTGCTC G[AT]GC[AT]^C
BanII 13 GGGCCC G[GA]GC[CT]^C
BbsI 1548 GAAGAC GAAGAC(2/6)
BcgI 639 GCACATGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 418 GGATAC GTATCC(6/5)
BglII 161 AGATCT A^GATCT
BlpI 248 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 248 GCTCAGC GC^T[ACGT]AGC
BpuAI 1548 GAAGAC GAAGAC(2/6)
BsaAI 240 CACGTA [CT]AC^GT[GA]
BsiEI 5 CGGCCG CG[GA][CT]^CG
BsiHKAI 154 GTGCTC G[AT]GC[AT]^C
BsrFI 140 GCCGGT [GA]^CCGG[CT]
Bst1107I 1765 GTATAC GTA^TAC
BstAPI 24 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 579 CCTGG CC^[AT]GG
BstZ17I 1765 GTATAC GTA^TAC
Cac8I 1189 GCTTGC GC[ACGT]^[ACGT]GC
CelII 248 GCTCAGC GC^T[ACGT]AGC
Cfr10I 140 GCCGGT [GA]^CCGG[CT]
DrdI 1372 GACGATTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 5 CGGCCG [CT]^GGCC[GA]
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
EcoRII 579 CCTGG ^CC[AT]GG
EcoRV 1780 GATATC GAT^ATC
EcoT14I 1101 CCATGG C^C[AT][AT]GG
EcoT22I 1508 ATGCAT ATGCA^T
FauI 18 CCCGC CCCGC(4/6)
HindIII 1366 AAGCTT A^AGCTT
MspA1I 1964 CAGCTG C[AC]G^C[GT]G
MvaI 579 CCTGG CC^[AT]GG
NcoI 1101 CCATGG C^CATGG
NsiI 1508 ATGCAT ATGCA^T
PflMI 906 CCATTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 958 GACAGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1082 CTGCAG CTGCA^G
PvuII 1964 CAGCTG CAG^CTG
SapI 1003 GCTCTTC GCTCTTC(1/4)
SspI 924 AATATT AAT^ATT
StyI 1101 CCATGG C^C[AT][AT]GG
Tsp45I 213 GTCAC ^GT[GC]AC
Van91I 906 CCATTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 103 GACGCC G[GA]^CG[CT]C
962 GGCGTC
AflII 768 CTTAAG C^TTAAG
1053 CTTAAG
AlwI 471 GGATC GGATC(4/5)
940 GGATC
AlwNI 1112 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
1961 CAGCAGCTG
AvaII 367 GGTCC G^G[AT]CC
822 GGACC
BanI 81 GGTGCC G^G[CT][GA]CC
319 GGTGCC
BcnI 78 CCGGG CC^[GC]GG
123 CCCGG
BfrI 768 CTTAAG C^TTAAG
1053 CTTAAG
BsaHI 103 GACGCC G[GA]^CG[CT]C
962 GGCGTC
BsaJI 8 CCGTGG C^C[ACGT][ACGT]GG
1101 CCATGG
BsmFI 821 GGGAC GGGAC(10/14)
368 GTCCC
Bsp1407I 761 TGTACA T^GTACA
1727 TGTACA
BspHI 1331 TCATGA T^CATGA
1653 TCATGA
BspLU11I 641 ACATGT A^CATGT
1064 ACATGT
BspMI 27 GCAGGT ACCTGC(4/8)
48 GCAGGT
BsrGI 761 TGTACA T^GTACA
1727 TGTACA
BstYI 161 AGATCT [GA]^GATC[CT]
471 GGATCT
DsaI 8 CCGTGG C^C[GA][CT]GG
1101 CCATGG
EcoNI 358 CCTCGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
484 CCTGTAATAGG
HaeII 60 GGCGCT [GA]GCGC^[CT]
72 GGCGCT
HgaI 103 GACGC GACGC(5/10)
963 GCGTC
Hin1I 103 GACGCC G[GA]^CG[CT]C
962 GGCGTC
HphI 282 GGTGA GGTGA(8/7)
633 GGTGA
MflI 161 AGATCT [GA]^GATC[CT]
471 GGATCT
MslI 1234 CATCACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1455 CACTATGATG
NciI 78 CCGGG CC^[GC]GG
123 CCCGG
PleI 716 GAGTC GAGTC(4/5)
1468 GAGTC
PpuMI 366 AGGTCCC [GA]G^G[AT]CC[CT]
821 GGGACCT
RcaI 1331 TCATGA T^CATGA
1653 TCATGA
SmlI 768 CTTAAG C^T[CT][GA]AG
1053 CTTAAG
SspBI 761 TGTACA T^GTACA
1727 TGTACA
XhoII 161 AGATCT [GA]^GATC[CT]
471 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI ApoI AseI AsnI BbvI
BmyI BpmI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MspI MwoI NdeII
NlaIII NlaIV NspI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I