Restriction map for
pF1KB7090
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AlwI
AlwNI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaWI BseAI BsiWI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I EarI Eco47III EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MaeI MaeIII
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
Tru9I Tsp45I TspRI Tth111I TthHB8I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AccII 333 CGCG CG^CG
AcsI 504 GAATTC [GA]^AATT[CT]
Alw44I 400 GTGCAC G^TGCAC
AocI 276 CCTGAGG CC^T[ACGT]AGG
ApaLI 400 GTGCAC G^TGCAC
ApoI 504 GAATTC [GA]^AATT[CT]
AvaII 482 GGTCC G^G[AT]CC
BanII 375 GAGCTC G[GA]GC[CT]^C
BbeI 327 GGCGCC GGCGC^C
BsaBI 150 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 445 GAGACC GGTCTC(1/5)
BsiEI 74 CGGCCG CG[GA][CT]^CG
BsmBI 454 GAGACG CGTCTC(1/5)
Bsp1407I 215 TGTACA T^GTACA
BspMI 288 GCAGGT ACCTGC(4/8)
BsrBI 229 GAGCGG CCGCTC(-3/-3)
BsrDI 92 GCAATG GCAATG(2/0)
BsrFI 76 GCCGGT [GA]^CCGG[CT]
BsrGI 215 TGTACA T^GTACA
BstF5I 495 CATCC GGATG(2/0)
BstNI 60 CCTGG CC^[AT]GG
BstUI 333 CGCG CG^CG
Bsu36I 276 CCTGAGG CC^T[ACGT]AGG
Cfr10I 76 GCCGGT [GA]^CCGG[CT]
DdeI 277 CTGAG C^T[ACGT]AG
DpnII 150 GATC ^GATC
DraII 481 GGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 74 CGGCCG [CT]^GGCC[GA]
EagI 74 CGGCCG C^GGCCG
EclXI 74 CGGCCG C^GGCCG
Eco52I 74 CGGCCG C^GGCCG
Eco81I 276 CCTGAGG CC^T[ACGT]AGG
EcoO109I 481 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 504 GAATTC G^AATTC
EcoRII 60 CCTGG ^CC[AT]GG
EcoT14I 356 CCATGG C^C[AT][AT]GG
FokI 495 CATCC GGATG(9/13)
HaeII 327 GGCGCC [GA]GCGC^[CT]
HgaI 456 GACGC GACGC(5/10)
HphI 407 TCACC GGTGA(8/7)
KasI 327 GGCGCC G^GCGCC
MaeII 398 ACGT A^CGT
MamI 150 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 150 GATC ^GATC
MboII 192 GAAGA GAAGA(8/7)
MvaI 60 CCTGG CC^[AT]GG
MvnI 333 CGCG CG^CG
NarI 327 GGCGCC GG^CGCC
NcoI 356 CCATGG C^CATGG
NdeII 150 GATC ^GATC
NspI 308 ACATGC [GA]CATG^[CT]
PpuMI 481 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 313 CTGCAG CTGCA^G
SacI 375 GAGCTC GAGCT^C
Sau3AI 150 GATC ^GATC
ScaI 104 AGTACT AGT^ACT
SfaNI 300 GATGC GCATC(5/9)
SfcI 313 CTGCAG C^T[GA][CT]AG
SfoI 327 GGCGCC GGC^GCC
SspBI 215 TGTACA T^GTACA
StyI 356 CCATGG C^C[AT][AT]GG
TfiI 155 GAATC G^A[AT]TC
Tsp509I 505 AATT ^AATT
XcmI 50 CCAGCAGCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 327 GGCGCC G[GA]^CG[CT]C
456 GACGCC
AfaI 105 GTAC GT^AC
216 GTAC
AluI 376 AGCT AG^CT
437 AGCT
AspHI 375 GAGCTC G[AT]GC[AT]^C
400 GTGCAC
BcnI 34 CCCGG CC^[GC]GG
393 CCCGG
BsaHI 327 GGCGCC G[GA]^CG[CT]C
456 GACGCC
BsgI 342 CTGCAC GTGCAG(16/14)
286 GTGCAG
BsiHKAI 375 GAGCTC G[AT]GC[AT]^C
400 GTGCAC
BsmAI 445 GAGAC GTCTC(1/5)
454 GAGAC
BsmFI 365 GGGAC GGGAC(10/14)
66 GTCCC
Cac8I 26 GCACGC GC[ACGT]^[ACGT]GC
330 GCCCGC
CfoI 328 GCGC GCG^C
334 GCGC
Cfr13I 273 GGGCC G^G[ACGT]CC
482 GGTCC
DsaI 263 CCGTGG C^C[GA][CT]GG
356 CCATGG
FauI 331 CCCGC CCCGC(4/6)
186 GCGGG
HhaI 328 GCGC GCG^C
334 GCGC
Hin1I 327 GGCGCC G[GA]^CG[CT]C
456 GACGCC
HinP1I 328 GCGC G^CGC
334 GCGC
MspA1I 87 CAGCGG C[AC]G^C[GT]G
256 CCGCTG
NciI 34 CCCGG CC^[GC]GG
393 CCCGG
PleI 64 GAGTC GAGTC(4/5)
382 GAGTC
RsaI 105 GTAC GT^AC
216 GTAC
Sau96I 273 GGGCC G^G[ACGT]CC
482 GGTCC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI BanI BbvI BmyI BpmI
BsaJI BseRI BsiYI BslI Bsp1286I
BssKI Fnu4HI HaeIII HapII HinfI
HpaII ItaI MnlI MspI MwoI
NlaIII NlaIV ScrFI TseI