################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvrII BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BsrI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI Eam1105I EarI Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeIII MamI MfeI MluI MroI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PleI PmeI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AcyI 63 GGCGTC G[GA]^CG[CT]C AfaI 126 GTAC GT^AC AflIII 434 ACGTGT A^C[GA][CT]GT AlwI 515 GGATC GGATC(4/5) AlwNI 513 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 361 CTCGAG C^[CT]CG[GA]G AviII 509 TGCGCA TGC^GCA BalI 290 TGGCCA TGG^CCA BanII 490 GAGCCC G[GA]GC[CT]^C BbrPI 433 CACGTG CAC^GTG BfaI 448 CTAG C^TAG BglI 292 GCCATGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 490 GAGCCC G[AGT]GC[ACT]^C BsaAI 433 CACGTG [CT]AC^GT[GA] BsaHI 63 GGCGTC G[GA]^CG[CT]C BseRI 244 GAGGAG GAGGAG(10/8) BsgI 379 CTGCAC GTGCAG(16/14) BsoBI 361 CTCGAG C^[CT]CG[GA]G Bsp1286I 490 GAGCCC G[AGT]GC[ACT]^C BsrBI 498 CCGCTC CCGCTC(-3/-3) BsrFI 335 ACCGGC [GA]^CCGG[CT] BssHII 280 GCGCGC G^CGCGC BstAPI 512 GCAGGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 515 GGATCT [GA]^GATC[CT] Cfr10I 335 ACCGGC [GA]^CCGG[CT] DdeI 52 CTGAG C^T[ACGT]AG DpnII 516 GATC ^GATC DraIII 313 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 405 GACCGCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 186 CCTGGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 68 CCAAGG C^C[AT][AT]GG FauI 484 CCCGC CCCGC(4/6) FspI 509 TGCGCA TGC^GCA HgaI 64 GCGTC GACGC(5/10) Hin1I 63 GGCGTC G[GA]^CG[CT]C MaeI 448 CTAG C^TAG MaeII 434 ACGT A^CGT MboI 516 GATC ^GATC MflI 515 GGATCT [GA]^GATC[CT] MluNI 290 TGGCCA TGG^CCA MscI 290 TGGCCA TGG^CCA MspA1I 232 CAGCTG C[AC]G^C[GT]G NdeII 516 GATC ^GATC NlaIII 294 CATG CATG^ PaeR7I 361 CTCGAG C^TCGAG PmaCI 433 CACGTG CAC^GTG PmlI 433 CACGTG CAC^GTG PpuMI 393 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 297 GACGGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 31 CTGCAG CTGCA^G PvuII 232 CAGCTG CAG^CTG RsaI 126 GTAC GT^AC Sau3AI 516 GATC ^GATC SfcI 31 CTGCAG C^T[GA][CT]AG SfiI 291 GGCCATGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StyI 68 CCAAGG C^C[AT][AT]GG XcmI 68 CCAAGGCAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 361 CTCGAG C^TCGAG XhoII 515 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AvaII 228 GGACC G^G[AT]CC 394 GGTCC BcnI 276 CCGGG CC^[GC]GG 353 CCGGG BspMI 374 ACCTGC ACCTGC(4/8) 506 ACCTGC BstXI 17 CCAAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 68 CCAAGGCAGTGG DraII 393 GGGTCCC [GA]G^G[ACGT]CC[CT] 524 AGGGCCT DsaI 389 CCGTGG C^C[GA][CT]GG 417 CCACGG EagI 299 CGGCCG C^GGCCG 420 CGGCCG EclXI 299 CGGCCG C^GGCCG 420 CGGCCG Eco52I 299 CGGCCG C^GGCCG 420 CGGCCG EcoO109I 393 GGGTCCC [GA]G^G[ACGT]CC[CT] 524 AGGGCCT HaeII 252 GGCGCT [GA]GCGC^[CT] 263 GGCGCT HphI 241 GGTGA GGTGA(8/7) 326 TCACC MboII 42 GAAGA GAAGA(8/7) 171 GAAGA NciI 276 CCGGG CC^[GC]GG 353 CCGGG PflMI 312 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 384 CCAGGCCGTGG SexAI 185 ACCTGGT A^CC[AT]GGT 314 ACCAGGT SmlI 178 CTCAAG C^T[CT][GA]AG 361 CTCGAG TaqI 332 TCGA T^CGA 362 TCGA TspRI 74 CAGTG CA[GC]TG[ACGT][ACGT]^ 105 CAGTG TthHB8I 332 TCGA T^CGA 362 TCGA Van91I 312 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 384 CCAGGCCGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AccII AciI AluI BbvI BsaJI BsiEI BsiYI BslI BsmFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I EaeI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspI MvaI MvnI MwoI NlaIV Sau96I ScrFI SfaNI TseI