Restriction map for
pF1KB7098
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HgaI HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MboII
MfeI MluI MluNI MroI MscI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TfiI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 547 GACGTC GACGT^C
AccI 504 GTCGAC GT^[AC][GT]AC
AccII 369 CGCG CG^CG
AcsI 382 AAATTC [GA]^AATT[CT]
AcyI 547 GACGTC G[GA]^CG[CT]C
AflIII 392 ACGTGT A^C[GA][CT]GT
Alw44I 325 GTGCAC G^TGCAC
ApaLI 325 GTGCAC G^TGCAC
ApoI 382 AAATTC [GA]^AATT[CT]
BanI 134 GGCACC G^G[CT][GA]CC
BanII 3 GAGCTC G[GA]GC[CT]^C
BbvI 334 GCTGC GCAGC(8/12)
BcnI 428 CCCGG CC^[GC]GG
BlpI 350 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 350 GCTCAGC GC^T[ACGT]AGC
BsaHI 547 GACGTC G[GA]^CG[CT]C
BspMI 160 ACCTGC ACCTGC(4/8)
BsrBI 348 CCGCTC CCGCTC(-3/-3)
BsrDI 78 CATTGC GCAATG(2/0)
BssSI 149 CTCGTG CACGAG(-5/-1)
BstAPI 155 GCATCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 50 GGATG GGATG(2/0)
BstUI 369 CGCG CG^CG
CelII 350 GCTCAGC GC^T[ACGT]AGC
CfoI 486 GCGC GCG^C
DdeI 351 CTCAG C^T[ACGT]AG
DsaI 275 CCGTGG C^C[GA][CT]GG
FokI 50 GGATG GGATG(9/13)
HaeII 485 AGCGCC [GA]GCGC^[CT]
HapII 429 CCGG C^CGG
HhaI 486 GCGC GCG^C
Hin1I 547 GACGTC G[GA]^CG[CT]C
HinP1I 486 GCGC G^CGC
HincII 504 GTCGAC GT[CT]^[GA]AC
HindII 504 GTCGAC GT[CT]^[GA]AC
HpaII 429 CCGG C^CGG
MaeIII 14 GTCAC ^GT[ACGT]AC
MslI 318 CACCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 429 CCGG C^CGG
MvnI 369 CGCG CG^CG
MwoI 155 GCATCACCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 428 CCCGG CC^[GC]GG
PstI 335 CTGCAG CTGCA^G
SacI 3 GAGCTC GAGCT^C
SalI 504 GTCGAC G^TCGAC
SfcI 335 CTGCAG C^T[GA][CT]AG
SphI 246 GCATGC GCATG^C
TseI 334 GCTGC G^C[AT]GC
Tsp45I 14 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 244 CCGC CCGC(-3/-1)
348 CCGC
AluI 4 AGCT AG^CT
491 AGCT
AlwI 186 GATCC GGATC(4/5)
288 GATCC
AspHI 3 GAGCTC G[AT]GC[AT]^C
325 GTGCAC
BmyI 3 GAGCTC G[AGT]GC[ACT]^C
325 GTGCAC
BseRI 93 CTCCTC GAGGAG(10/8)
139 CTCCTC
BsiHKAI 3 GAGCTC G[AT]GC[AT]^C
325 GTGCAC
BsmAI 65 GAGAC GTCTC(1/5)
564 GTCTC
Bsp1286I 3 GAGCTC G[AGT]GC[ACT]^C
325 GTGCAC
BstNI 105 CCTGG CC^[AT]GG
144 CCAGG
BstXI 144 CCAGGCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
489 CCAGCTCTTTGG
BstYI 185 AGATCC [GA]^GATC[CT]
287 AGATCC
DpnII 186 GATC ^GATC
288 GATC
EcoNI 138 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
399 CCTGCTGGAGG
EcoRII 105 CCTGG ^CC[AT]GG
144 CCAGG
EcoT14I 28 CCAAGG C^C[AT][AT]GG
320 CCTTGG
HinfI 33 GACTC G^A[ACGT]TC
562 GAGTC
MboI 186 GATC ^GATC
288 GATC
MflI 185 AGATCC [GA]^GATC[CT]
287 AGATCC
MvaI 105 CCTGG CC^[AT]GG
144 CCAGG
NdeII 186 GATC ^GATC
288 GATC
NspI 239 ACATGC [GA]CATG^[CT]
246 GCATGC
PleI 33 GACTC GAGTC(4/5)
562 GAGTC
Sau3AI 186 GATC ^GATC
288 GATC
SfaNI 576 GATGC GCATC(5/9)
155 GCATC
StyI 28 CCAAGG C^C[AT][AT]GG
320 CCTTGG
TaqI 505 TCGA T^CGA
567 TCGA
TthHB8I 505 TCGA T^CGA
567 TCGA
XhoII 185 AGATCC [GA]^GATC[CT]
287 AGATCC
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AfaI BpmI BsaJI BsiYI BslI
BsrI BssKI Cac8I Cfr13I DraIII
Fnu4HI HaeIII HphI ItaI MaeII
MnlI MseI NlaIII NlaIV RsaI
Sau96I ScrFI Tru9I Tsp509I TspRI