Restriction map for
pF1KB7105
##################################
# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AlwNI
AocI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
KspI MaeI MaeII MamI MfeI
MluI MroI MseI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI TfiI
Tru9I Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccII 31 CGCG CG^CG
AcsI 390 AAATTC [GA]^AATT[CT]
AcyI 221 GGCGCC G[GA]^CG[CT]C
AfaI 424 GTAC GT^AC
Alw44I 203 GTGCAC G^TGCAC
Aor51HI 269 AGCGCT AGC^GCT
ApaLI 203 GTGCAC G^TGCAC
ApoI 390 AAATTC [GA]^AATT[CT]
BalI 113 TGGCCA TGG^CCA
BamHI 585 GGATCC G^GATCC
BbeI 221 GGCGCC GGCGC^C
BbvCI 380 CCTCAGC CCTCAGC(-5/-2)
BglII 626 AGATCT A^GATCT
BsaHI 221 GGCGCC G[GA]^CG[CT]C
BseRI 230 GAGGAG GAGGAG(10/8)
BspMI 372 ACCTGC ACCTGC(4/8)
BstEII 612 GGTCACC G^GT[ACGT]ACC
BstPI 612 GGTCACC G^GT[ACGT]ACC
BstUI 31 CGCG CG^CG
Cac8I 532 GCCAGC GC[ACGT]^[ACGT]GC
EarI 130 CTCTTC CTCTTC(1/4)
Eco47III 269 AGCGCT AGC^GCT
EcoO65I 612 GGTCACC G^GT[ACGT]ACC
HgaI 536 GCGTC GACGC(5/10)
Hin1I 221 GGCGCC G[GA]^CG[CT]C
HincII 6 GTTGAC GT[CT]^[GA]AC
HindII 6 GTTGAC GT[CT]^[GA]AC
HinfI 9 GACTC G^A[ACGT]TC
KasI 221 GGCGCC G^GCGCC
Ksp632I 130 CTCTTC CTCTTC(1/4)
MaeIII 613 GTCAC ^GT[ACGT]AC
MboII 131 TCTTC GAAGA(8/7)
MluNI 113 TGGCCA TGG^CCA
MscI 113 TGGCCA TGG^CCA
MvnI 31 CGCG CG^CG
NarI 221 GGCGCC GG^CGCC
PleI 9 GACTC GAGTC(4/5)
PpuMI 431 AGGTCCC [GA]G^G[AT]CC[CT]
RsaI 424 GTAC GT^AC
SapI 129 GCTCTTC GCTCTTC(1/4)
SfaNI 92 GCATC GCATC(5/9)
SfoI 221 GGCGCC GGC^GCC
StuI 492 AGGCCT AGG^CCT
Tsp45I 613 GTCAC ^GT[GC]AC
Tsp509I 391 AATT ^AATT
XcmI 143 CCACCGCTGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwI 586 GATCC GGATC(4/5)
585 GGATC
BanI 221 GGCGCC G^G[CT][GA]CC
256 GGCACC
BcnI 440 CCCGG CC^[GC]GG
582 CCCGG
BsmFI 298 GTCCC GGGAC(10/14)
433 GTCCC
BstF5I 93 CATCC GGATG(2/0)
135 CATCC
BstYI 585 GGATCC [GA]^GATC[CT]
626 AGATCT
EaeI 113 TGGCCA [CT]^GGCC[GA]
571 CGGCCA
FauI 46 CCCGC CCCGC(4/6)
484 CCCGC
FokI 93 CATCC GGATG(9/13)
135 CATCC
HaeII 221 GGCGCC [GA]GCGC^[CT]
269 AGCGCT
MflI 585 GGATCC [GA]^GATC[CT]
626 AGATCT
MspA1I 146 CCGCTG C[AC]G^C[GT]G
568 CAGCGG
NciI 440 CCCGG CC^[GC]GG
582 CCCGG
NcoI 64 CCATGG C^CATGG
251 CCATGG
TspRI 74 CACTG CA[GC]TG[ACGT][ACGT]^
660 CAGTG
XhoII 585 GGATCC [GA]^GATC[CT]
626 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI ApaI AspHI AvaI
AvaII BanII BbvI BglI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsoBI Bsp1286I BsrI BssKI BstNI
BstXI CfoI Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRII EcoT14I
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MboI MnlI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
StyI TseI