Restriction map for
pF1KB7106
##################################
# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvaI AviII AvrII BamHI BanI
BbeI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BsaBI BsaHI BsaI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DdeI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HinfI HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflMI PinAI PleI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Van91I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AflIII 185 ACGTGT A^C[GA][CT]GT
AluI 239 AGCT AG^CT
Aor51HI 261 AGCGCT AGC^GCT
ApaI 230 GGGCCC GGGCC^C
AspI 148 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 272 TGGCCA TGG^CCA
BanII 230 GGGCCC G[GA]GC[CT]^C
BbrPI 280 CACGTG CAC^GTG
BfaI 268 CTAG C^TAG
BglII 65 AGATCT A^GATCT
BsaAI 280 CACGTG [CT]AC^GT[GA]
BsaWI 290 ACCGGA [AT]^CCGG[AT]
BseRI 355 GAGGAG GAGGAG(10/8)
BsmAI 294 GAGAC GTCTC(1/5)
BspHI 581 TCATGA T^CATGA
BsrI 673 CCAGT ACTGG(1/-1)
BstBI 436 TTCGAA TT^CGAA
BstYI 65 AGATCT [GA]^GATC[CT]
CfoI 262 GCGC GCG^C
DraII 230 GGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 554 CCACGG C^C[GA][CT]GG
EaeI 272 TGGCCA [CT]^GGCC[GA]
Eco47III 261 AGCGCT AGC^GCT
EcoO109I 230 GGGCCCT [GA]G^G[ACGT]CC[CT]
HaeII 261 AGCGCT [GA]GCGC^[CT]
HhaI 262 GCGC GCG^C
HinP1I 262 GCGC G^CGC
MaeI 268 CTAG C^TAG
MaeIII 619 GTCAC ^GT[ACGT]AC
MflI 65 AGATCT [GA]^GATC[CT]
MluNI 272 TGGCCA TGG^CCA
MscI 272 TGGCCA TGG^CCA
MseI 344 TTAA T^TAA
MslI 276 CATGCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 380 GCATCGAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspV 436 TTCGAA TT^CGAA
PflFI 148 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 280 CACGTG CAC^GTG
PmlI 280 CACGTG CAC^GTG
PvuII 238 CAGCTG CAG^CTG
RcaI 581 TCATGA T^CATGA
SfuI 436 TTCGAA TT^CGAA
Tru9I 344 TTAA T^TAA
Tsp45I 619 GTCAC ^GT[GC]AC
Tth111I 148 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 485 CCATGCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 65 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 340 GAATTT [GA]^AATT[CT]
599 GAATTT
AfaI 453 GTAC GT^AC
471 GTAC
AlwI 24 GATCC GGATC(4/5)
102 GATCC
AlwNI 490 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
590 CAGTGTCTG
ApoI 340 GAATTT [GA]^AATT[CT]
599 GAATTT
AvaII 375 GGTCC G^G[AT]CC
401 GGACC
BcnI 426 CCGGG CC^[GC]GG
548 CCCGG
BmyI 9 GGGCAC G[AGT]GC[ACT]^C
230 GGGCCC
BsaJI 27 CCTGGG C^C[ACGT][ACGT]GG
554 CCACGG
BsmFI 521 GTCCC GGGAC(10/14)
546 GTCCC
Bsp1286I 9 GGGCAC G[AGT]GC[ACT]^C
230 GGGCCC
BstF5I 219 CATCC GGATG(2/0)
606 CATCC
BstXI 275 CCATGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
524 CCAACACTGTGG
EarI 82 CTCTTC CTCTTC(1/4)
306 GAAGAG
FokI 219 CATCC GGATG(9/13)
606 CATCC
HphI 498 GGTGA GGTGA(8/7)
74 TCACC
Ksp632I 82 CTCTTC CTCTTC(1/4)
306 GAAGAG
MspA1I 238 CAGCTG C[AC]G^C[GT]G
490 CAGCGG
NciI 426 CCGGG CC^[GC]GG
548 CCCGG
NlaIV 230 GGGCCC GG[ACGT]^[ACGT]CC
388 GGCTCC
RsaI 453 GTAC GT^AC
471 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI BbsI BbvI BpuAI BsiYI
BslI BssKI BstNI Cac8I Cfr13I
DpnII EcoRII Fnu4HI HaeIII HapII
HpaII ItaI MaeII MboI MboII
MnlI MspI MvaI NdeII NlaIII
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TseI Tsp509I TspRI
TthHB8I