Restriction map for
pF1KB7110
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaI ApaLI
ApoI AscI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbrPI BbvCI BciVI BclI BcnI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsoBI Bsp1407I BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstXI BstZ17I CelII Cfr10I
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FokI FseI FspI HapII HgaI
HincII HindII HindIII HpaI HpaII
KpnI KspI MamI MfeI MluI
MroI MspI MunI NaeI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Tsp509I Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 382 CGCG CG^CG
AcyI 88 GGCGCC G[GA]^CG[CT]C
AlwNI 268 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 211 CCTGAGG CC^T[ACGT]AGG
AseI 765 ATTAAT AT^TAAT
AsnI 765 ATTAAT AT^TAAT
AspHI 304 GAGCTC G[AT]GC[AT]^C
BalI 566 TGGCCA TGG^CCA
BanII 304 GAGCTC G[GA]GC[CT]^C
BbeI 88 GGCGCC GGCGC^C
BcgI 318 CGAGAAGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 85 CTAG C^TAG
BglII 395 AGATCT A^GATCT
BsaHI 88 GGCGCC G[GA]^CG[CT]C
BsiHKAI 304 GAGCTC G[AT]GC[AT]^C
BsmFI 122 GGGAC GGGAC(10/14)
BsmI 324 GAATGC GAATGC(1/-1)
BspDI 712 ATCGAT AT^CGAT
BspMI 206 ACCTGC ACCTGC(4/8)
BstAPI 449 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 382 CGCG CG^CG
BstYI 395 AGATCT [GA]^GATC[CT]
Bsu36I 211 CCTGAGG CC^T[ACGT]AGG
ClaI 712 ATCGAT AT^CGAT
EaeI 566 TGGCCA [CT]^GGCC[GA]
Eco81I 211 CCTGAGG CC^T[ACGT]AGG
HaeII 88 GGCGCC [GA]GCGC^[CT]
Hin1I 88 GGCGCC G[GA]^CG[CT]C
KasI 88 GGCGCC G^GCGCC
MaeI 85 CTAG C^TAG
MaeII 23 ACGT A^CGT
MaeIII 152 GTGAC ^GT[ACGT]AC
MflI 395 AGATCT [GA]^GATC[CT]
MluNI 566 TGGCCA TGG^CCA
MscI 566 TGGCCA TGG^CCA
MseI 766 TTAA T^TAA
MslI 724 CACCCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 99 CAGCGG C[AC]G^C[GT]G
MvnI 382 CGCG CG^CG
NarI 88 GGCGCC GG^CGCC
NspI 729 ACATGC [GA]CATG^[CT]
PflMI 222 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 536 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 765 ATTAAT AT^TAAT
PstI 286 CTGCAG CTGCA^G
SacI 304 GAGCTC GAGCT^C
SapI 486 GCTCTTC GCTCTTC(1/4)
SbfI 285 CCTGCAGG CCTGCA^GG
SexAI 260 ACCAGGT A^CC[AT]GGT
SfoI 88 GGCGCC GGC^GCC
Sse8387I 285 CCTGCAGG CCTGCA^GG
TfiI 715 GATTC G^A[AT]TC
Tru9I 766 TTAA T^TAA
Tsp45I 152 GTGAC ^GT[GC]AC
Van91I 222 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 395 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
BbsI 427 GTCTTC GAAGAC(2/6)
664 GTCTTC
BbvI 267 GCAGC GCAGC(8/12)
234 GCTGC
BmyI 304 GAGCTC G[AGT]GC[ACT]^C
631 GTGCCC
BpuAI 427 GTCTTC GAAGAC(2/6)
664 GTCTTC
BsgI 265 GTGCAG GTGCAG(16/14)
358 GTGCAG
Bsp1286I 304 GAGCTC G[AGT]GC[ACT]^C
631 GTGCCC
BsrI 51 ACTGG ACTGG(1/-1)
560 ACTGG
Cac8I 581 GCCTGC GC[ACGT]^[ACGT]GC
633 GCCCGC
CfoI 89 GCGC GCG^C
381 GCGC
DpnII 396 GATC ^GATC
648 GATC
EcoT14I 311 CCAAGG C^C[AT][AT]GG
474 CCTTGG
FauI 634 CCCGC CCCGC(4/6)
101 GCGGG
Fnu4HI 234 GCTGC GC^[ACGT]GC
267 GCAGC
HhaI 89 GCGC GCG^C
381 GCGC
HinP1I 89 GCGC G^CGC
381 GCGC
HphI 521 TCACC GGTGA(8/7)
723 TCACC
ItaI 234 GCTGC GC^[ACGT]GC
267 GCAGC
MboI 396 GATC ^GATC
648 GATC
MwoI 449 GCACCTCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
497 GCTCCAATGGC
NdeII 396 GATC ^GATC
648 GATC
PleI 76 GACTC GAGTC(4/5)
552 GACTC
Sau3AI 396 GATC ^GATC
648 GATC
SfaNI 108 GATGC GCATC(5/9)
710 GCATC
SfcI 286 CTGCAG C^T[GA][CT]AG
399 CTACAG
SmlI 466 CTCAAG C^T[CT][GA]AG
613 CTCAAG
StyI 311 CCAAGG C^C[AT][AT]GG
474 CCTTGG
TaqI 713 TCGA T^CGA
762 TCGA
TseI 234 GCTGC G^C[AT]GC
267 GCAGC
TthHB8I 713 TCGA T^CGA
762 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BsaJI BseRI BsiYI BslI BssKI
BstNI Cfr13I DdeI DraII EarI
EcoNI EcoO109I EcoRII HaeIII HinfI
Ksp632I MboII MnlI MvaI NlaIII
NlaIV RsaI Sau96I ScrFI TspRI