Restriction map for
pF1KB7113
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HinfI HpaI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PleI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccI 103 GTATAC GT^[AC][GT]AC
AcyI 577 GGCGCC G[GA]^CG[CT]C
ApaI 230 GGGCCC GGGCC^C
Asp700I 552 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 613 GAGCTC G[AT]GC[AT]^C
AvaI 668 CTCGAG C^[CT]CG[GA]G
BamHI 753 GGATCC G^GATCC
BbeI 577 GGCGCC GGCGC^C
BbrPI 280 CACGTG CAC^GTG
BcgI 670 CGAGGGGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 493 GCCAACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 16 CTCCAG CTGGAG(16/14)
BsaHI 577 GGCGCC G[GA]^CG[CT]C
BseRI 295 GAGGAG GAGGAG(10/8)
BsiHKAI 613 GAGCTC G[AT]GC[AT]^C
BsmFI 329 GGGAC GGGAC(10/14)
BsoBI 668 CTCGAG C^[CT]CG[GA]G
BsrDI 531 CATTGC GCAATG(2/0)
BsrFI 308 GCCGGC [GA]^CCGG[CT]
Bst1107I 103 GTATAC GTA^TAC
BstXI 512 CCAACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 753 GGATCC [GA]^GATC[CT]
BstZ17I 103 GTATAC GTA^TAC
Cfr10I 308 GCCGGC [GA]^CCGG[CT]
DdeI 28 CTGAG C^T[ACGT]AG
DpnII 754 GATC ^GATC
DsaI 77 CCGTGG C^C[GA][CT]GG
EarI 430 CTCTTC CTCTTC(1/4)
EcoNI 651 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 300 GCGGG CCCGC(4/6)
Hin1I 577 GGCGCC G[GA]^CG[CT]C
KasI 577 GGCGCC G^GCGCC
Ksp632I 430 CTCTTC CTCTTC(1/4)
MaeIII 250 GTCAC ^GT[ACGT]AC
MboI 754 GATC ^GATC
MflI 753 GGATCC [GA]^GATC[CT]
MspA1I 717 CCGCTG C[AC]G^C[GT]G
NaeI 308 GCCGGC GCC^GGC
NarI 577 GGCGCC GG^CGCC
NdeII 754 GATC ^GATC
NgoMIV 308 GCCGGC G^CCGGC
NspI 253 ACATGC [GA]CATG^[CT]
PaeR7I 668 CTCGAG C^TCGAG
PflMI 726 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 280 CACGTG CAC^GTG
PmlI 280 CACGTG CAC^GTG
PpuMI 638 GGGTCCT [GA]G^G[AT]CC[CT]
SacI 613 GAGCTC GAGCT^C
Sau3AI 754 GATC ^GATC
ScaI 44 AGTACT AGT^ACT
SfoI 577 GGCGCC GGC^GCC
SmlI 668 CTCGAG C^T[CT][GA]AG
TaqI 669 TCGA T^CGA
Tsp45I 250 GTCAC ^GT[GC]AC
TthHB8I 669 TCGA T^CGA
Van91I 726 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 668 CTCGAG C^TCGAG
XhoII 753 GGATCC [GA]^GATC[CT]
XmnI 552 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 124 CGCG CG^CG
476 CGCG
AlwI 754 GATCC GGATC(4/5)
753 GGATC
BanI 311 GGCACC G^G[CT][GA]CC
577 GGCGCC
BanII 230 GGGCCC G[GA]GC[CT]^C
613 GAGCTC
BbsI 9 GAAGAC GAAGAC(2/6)
88 GTCTTC
BcnI 93 CCGGG CC^[GC]GG
172 CCCGG
BmyI 230 GGGCCC G[AGT]GC[ACT]^C
613 GAGCTC
BpuAI 9 GAAGAC GAAGAC(2/6)
88 GTCTTC
BsaAI 106 TACGTG [CT]AC^GT[GA]
280 CACGTG
BsaJI 77 CCGTGG C^C[ACGT][ACGT]GG
762 CCCAGG
Bsp1286I 230 GGGCCC G[AGT]GC[ACT]^C
613 GAGCTC
BspMI 440 ACCTGC ACCTGC(4/8)
712 ACCTGC
BsrBI 30 GAGCGG CCGCTC(-3/-3)
298 GAGCGG
BsrI 5 ACTGG ACTGG(1/-1)
333 CCAGT
BstUI 124 CGCG CG^CG
476 CGCG
HaeII 305 AGCGCC [GA]GCGC^[CT]
577 GGCGCC
HphI 560 TCACC GGTGA(8/7)
602 TCACC
MvnI 124 CGCG CG^CG
476 CGCG
NciI 93 CCGGG CC^[GC]GG
172 CCCGG
SfaNI 65 GCATC GCATC(5/9)
593 GCATC
StuI 375 AGGCCT AGG^CCT
648 AGGCCT
TspRI 334 CAGTG CA[GC]TG[ACGT][ACGT]^
487 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BsgI BsiYI BslI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DraII
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeII MboII MnlI MspI MvaI
MwoI NlaIII NlaIV PstI RsaI
Sau96I ScrFI SfcI TseI