################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BbvCI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI Bsu36I CelII ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HinfI HpaI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccI 103 GTATAC GT^[AC][GT]AC AcyI 577 GGCGCC G[GA]^CG[CT]C ApaI 230 GGGCCC GGGCC^C Asp700I 552 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 613 GAGCTC G[AT]GC[AT]^C AvaI 668 CTCGAG C^[CT]CG[GA]G BamHI 753 GGATCC G^GATCC BbeI 577 GGCGCC GGCGC^C BbrPI 280 CACGTG CAC^GTG BcgI 670 CGAGGGGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 493 GCCAACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 16 CTCCAG CTGGAG(16/14) BsaHI 577 GGCGCC G[GA]^CG[CT]C BseRI 295 GAGGAG GAGGAG(10/8) BsiHKAI 613 GAGCTC G[AT]GC[AT]^C BsmFI 329 GGGAC GGGAC(10/14) BsoBI 668 CTCGAG C^[CT]CG[GA]G BsrDI 531 CATTGC GCAATG(2/0) BsrFI 308 GCCGGC [GA]^CCGG[CT] Bst1107I 103 GTATAC GTA^TAC BstXI 512 CCAACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 753 GGATCC [GA]^GATC[CT] BstZ17I 103 GTATAC GTA^TAC Cfr10I 308 GCCGGC [GA]^CCGG[CT] DdeI 28 CTGAG C^T[ACGT]AG DpnII 754 GATC ^GATC DsaI 77 CCGTGG C^C[GA][CT]GG EarI 430 CTCTTC CTCTTC(1/4) EcoNI 651 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 300 GCGGG CCCGC(4/6) Hin1I 577 GGCGCC G[GA]^CG[CT]C KasI 577 GGCGCC G^GCGCC Ksp632I 430 CTCTTC CTCTTC(1/4) MaeIII 250 GTCAC ^GT[ACGT]AC MboI 754 GATC ^GATC MflI 753 GGATCC [GA]^GATC[CT] MspA1I 717 CCGCTG C[AC]G^C[GT]G NaeI 308 GCCGGC GCC^GGC NarI 577 GGCGCC GG^CGCC NdeII 754 GATC ^GATC NgoMIV 308 GCCGGC G^CCGGC NspI 253 ACATGC [GA]CATG^[CT] PaeR7I 668 CTCGAG C^TCGAG PflMI 726 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 280 CACGTG CAC^GTG PmlI 280 CACGTG CAC^GTG PpuMI 638 GGGTCCT [GA]G^G[AT]CC[CT] SacI 613 GAGCTC GAGCT^C Sau3AI 754 GATC ^GATC ScaI 44 AGTACT AGT^ACT SfoI 577 GGCGCC GGC^GCC SmlI 668 CTCGAG C^T[CT][GA]AG TaqI 669 TCGA T^CGA Tsp45I 250 GTCAC ^GT[GC]AC TthHB8I 669 TCGA T^CGA Van91I 726 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 668 CTCGAG C^TCGAG XhoII 753 GGATCC [GA]^GATC[CT] XmnI 552 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccII 124 CGCG CG^CG 476 CGCG AlwI 754 GATCC GGATC(4/5) 753 GGATC BanI 311 GGCACC G^G[CT][GA]CC 577 GGCGCC BanII 230 GGGCCC G[GA]GC[CT]^C 613 GAGCTC BbsI 9 GAAGAC GAAGAC(2/6) 88 GTCTTC BcnI 93 CCGGG CC^[GC]GG 172 CCCGG BmyI 230 GGGCCC G[AGT]GC[ACT]^C 613 GAGCTC BpuAI 9 GAAGAC GAAGAC(2/6) 88 GTCTTC BsaAI 106 TACGTG [CT]AC^GT[GA] 280 CACGTG BsaJI 77 CCGTGG C^C[ACGT][ACGT]GG 762 CCCAGG Bsp1286I 230 GGGCCC G[AGT]GC[ACT]^C 613 GAGCTC BspMI 440 ACCTGC ACCTGC(4/8) 712 ACCTGC BsrBI 30 GAGCGG CCGCTC(-3/-3) 298 GAGCGG BsrI 5 ACTGG ACTGG(1/-1) 333 CCAGT BstUI 124 CGCG CG^CG 476 CGCG HaeII 305 AGCGCC [GA]GCGC^[CT] 577 GGCGCC HphI 560 TCACC GGTGA(8/7) 602 TCACC MvnI 124 CGCG CG^CG 476 CGCG NciI 93 CCGGG CC^[GC]GG 172 CCCGG SfaNI 65 GCATC GCATC(5/9) 593 GCATC StuI 375 AGGCCT AGG^CCT 648 AGGCCT TspRI 334 CAGTG CA[GC]TG[ACGT][ACGT]^ 487 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AfaI AluI AvaII BbvI BsgI BsiYI BslI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MboII MnlI MspI MvaI MwoI NlaIII NlaIV PstI RsaI Sau96I ScrFI SfcI TseI