Restriction map for
pF1KB7119
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BbsI BcgI BciVI BclI
BfrI BglII BlnI BpuAI BsaAI
BsaI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrGI BsrI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HindIII
HpaI KpnI KspI MaeIII MfeI
MluI MluNI MroI MscI MseI
MunI NaeI NcoI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SbfI ScaI SexAI SfcI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp45I Tsp509I Tth111I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 757 GTCGAC GT^[AC][GT]AC
AcyI 230 GGCGCC G[GA]^CG[CT]C
AlwI 474 GATCC GGATC(4/5)
AlwNI 166 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
BanI 230 GGCGCC G^G[CT][GA]CC
BbeI 230 GGCGCC GGCGC^C
BfaI 13 CTAG C^TAG
BglI 274 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 327 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 327 GCTGAGC GC^T[ACGT]AGC
BsaBI 474 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 230 GGCGCC G[GA]^CG[CT]C
BseRI 808 GAGGAG GAGGAG(10/8)
BsgI 799 GTGCAG GTGCAG(16/14)
BsiEI 755 CGGTCG CG[GA][CT]^CG
BsmBI 106 GAGACG CGTCTC(1/5)
BspLU11I 536 ACATGT A^CATGT
BspMI 783 GCAGGT ACCTGC(4/8)
BstYI 473 AGATCC [GA]^GATC[CT]
CelII 327 GCTGAGC GC^T[ACGT]AGC
EarI 679 CTCTTC CTCTTC(1/4)
Hin1I 230 GGCGCC G[GA]^CG[CT]C
HincII 757 GTCGAC GT[CT]^[GA]AC
HindII 757 GTCGAC GT[CT]^[GA]AC
KasI 230 GGCGCC G^GCGCC
Ksp632I 679 CTCTTC CTCTTC(1/4)
MaeI 13 CTAG C^TAG
MamI 474 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 473 AGATCC [GA]^GATC[CT]
MslI 219 CACGTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 230 GGCGCC GG^CGCC
NheI 12 GCTAGC G^CTAGC
NspI 536 ACATGT [GA]CATG^[CT]
SalI 757 GTCGAC G^TCGAC
SapI 678 GCTCTTC GCTCTTC(1/4)
SfiI 273 GGCCATCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 230 GGCGCC GGC^GCC
SmaI 284 CCCGGG CCC^GGG
SmlI 856 CTCAAG C^T[CT][GA]AG
XhoII 473 AGATCC [GA]^GATC[CT]
XmaCI 284 CCCGGG C^CCGGG
XmaI 284 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 536 ACATGT A^C[GA][CT]GT
560 ACGTGT
AspHI 676 GAGCTC G[AT]GC[AT]^C
736 GAGCTC
AvaI 284 CCCGGG C^[CT]CG[GA]G
516 CTCGGG
AvaII 111 GGACC G^G[AT]CC
827 GGACC
BanII 676 GAGCTC G[GA]GC[CT]^C
736 GAGCTC
BbvCI 234 CCTCAGC CCTCAGC(-5/-2)
340 GCTGAGG
BmyI 676 GAGCTC G[AGT]GC[ACT]^C
736 GAGCTC
BsiHKAI 676 GAGCTC G[AT]GC[AT]^C
736 GAGCTC
BsoBI 284 CCCGGG C^[CT]CG[GA]G
516 CTCGGG
Bsp1286I 676 GAGCTC G[AGT]GC[ACT]^C
736 GAGCTC
BsrFI 8 ACCGGC [GA]^CCGG[CT]
766 ACCGGC
BssSI 88 CACGAG CACGAG(-5/-1)
673 CACGAG
Cfr10I 8 ACCGGC [GA]^CCGG[CT]
766 ACCGGC
EcoT14I 269 CCAAGG C^C[AT][AT]GG
776 CCAAGG
HaeII 230 GGCGCC [GA]GCGC^[CT]
485 AGCGCC
HinfI 79 GACTC G^A[ACGT]TC
124 GAGTC
HphI 404 TCACC GGTGA(8/7)
410 TCACC
MboII 813 GAAGA GAAGA(8/7)
680 TCTTC
MspA1I 166 CAGCTG C[AC]G^C[GT]G
490 CAGCTG
NlaIV 230 GGCGCC GG[ACGT]^[ACGT]CC
827 GGACCC
PflMI 114 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
165 CCAGCTGCTGG
PleI 79 GACTC GAGTC(4/5)
124 GAGTC
PvuII 166 CAGCTG CAG^CTG
490 CAGCTG
SacI 676 GAGCTC GAGCT^C
736 GAGCTC
StyI 269 CCAAGG C^C[AT][AT]GG
776 CCAAGG
TspRI 301 CACTG CA[GC]TG[ACGT][ACGT]^
368 CAGTG
Van91I 114 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
165 CCAGCTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccII AciI AfaI AluI BbvI
BcnI BpmI BsaJI BsiYI BslI
BsmAI BsmFI BssHII BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII ItaI MaeII MboI
MnlI MspI MvaI MvnI MwoI
NciI NdeII NlaIII RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TseI
TthHB8I