Restriction map for
pF1KB7138
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
Acc65I AccII AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaII AvrII BamHI
BanI BbeI BcgI BciVI BcnI
BfaI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmAI BsmBI
BsmI BspDI BspEI BspLU11I BsrBI
BsrFI BsrI BssHII BssSI Bst1107I
BstAPI BstUI BstYI BstZ17I Bsu36I
CelII Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT14I
EcoT22I FseI HaeII HapII HgaI
HindIII HpaII KasI KpnI KspI
MaeI MfeI MflI MluI MroI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacII SalI SapI
Sau96I SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI StuI StyI SwaI Tth111I
Van91I XbaI XcmI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 654 GACGTC GACGT^C
AccI 956 GTCTAC GT^[AC][GT]AC
AciI 506 CCGC CCGC(-3/-1)
AcyI 654 GACGTC G[GA]^CG[CT]C
AlwI 695 GGATC GGATC(4/5)
AvaI 884 CTCGAG C^[CT]CG[GA]G
AviII 929 TGCGCA TGC^GCA
BanII 293 GAGCTC G[GA]GC[CT]^C
BbsI 79 GTCTTC GAAGAC(2/6)
BbvCI 828 CCTCAGC CCTCAGC(-5/-2)
BclI 2 TGATCA T^GATCA
BglI 412 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 79 GTCTTC GAAGAC(2/6)
BsaBI 696 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 654 GACGTC G[GA]^CG[CT]C
BsgI 715 GTGCAG GTGCAG(16/14)
BsiYI 762 CCTGATGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 762 CCTGATGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 31 GTCCC GGGAC(10/14)
BsoBI 884 CTCGAG C^[CT]CG[GA]G
Bsp1407I 369 TGTACA T^GTACA
BsrDI 318 CATTGC GCAATG(2/0)
BsrGI 369 TGTACA T^GTACA
BssKI 797 CCTGG ^CC[ACGT]GG
BstEII 168 GGTAACC G^GT[ACGT]ACC
BstNI 797 CCTGG CC^[AT]GG
BstPI 168 GGTAACC G^GT[ACGT]ACC
BstXI 282 CCAGATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 930 GCGC GCG^C
DsaI 164 CCACGG C^C[GA][CT]GG
EarI 156 GAAGAG CTCTTC(1/4)
EcoO65I 168 GGTAACC G^GT[ACGT]ACC
EcoRII 797 CCTGG ^CC[AT]GG
FauI 505 CCCGC CCCGC(4/6)
FbaI 2 TGATCA T^GATCA
FspI 929 TGCGCA TGC^GCA
HhaI 930 GCGC GCG^C
Hin1I 654 GACGTC G[GA]^CG[CT]C
HinP1I 930 GCGC G^CGC
HincII 115 GTTAAC GT[CT]^[GA]AC
HindII 115 GTTAAC GT[CT]^[GA]AC
HpaI 115 GTTAAC GTT^AAC
HphI 5 TCACC GGTGA(8/7)
Ksp632I 156 GAAGAG CTCTTC(1/4)
MamI 696 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvaI 797 CCTGG CC^[AT]GG
NlaIV 802 GGAGCC GG[ACGT]^[ACGT]CC
NspI 926 GCATGC [GA]CATG^[CT]
PaeR7I 884 CTCGAG C^TCGAG
SacI 293 GAGCTC GAGCT^C
ScaI 272 AGTACT AGT^ACT
ScrFI 797 CCTGG CC^[ACGT]GG
SfaNI 765 GATGC GCATC(5/9)
SfcI 84 CTATAG C^T[GA][CT]AG
SphI 926 GCATGC GCATG^C
SspBI 369 TGTACA T^GTACA
Tsp45I 963 GTCAC ^GT[GC]AC
XhoI 884 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 401 ACACGT A^C[GA][CT]GT
835 ACGTGT
AspHI 293 GAGCTC G[AT]GC[AT]^C
721 GTGCTC
BalI 259 TGGCCA TGG^CCA
362 TGGCCA
BbrPI 402 CACGTG CAC^GTG
834 CACGTG
BbvI 70 GCAGC GCAGC(8/12)
708 GCTGC
BmyI 293 GAGCTC G[AGT]GC[ACT]^C
721 GTGCTC
BsaAI 402 CACGTG [CT]AC^GT[GA]
834 CACGTG
BsiHKAI 293 GAGCTC G[AT]GC[AT]^C
721 GTGCTC
Bsp1286I 293 GAGCTC G[AGT]GC[ACT]^C
721 GTGCTC
BspHI 605 TCATGA T^CATGA
821 TCATGA
BspMI 511 ACCTGC ACCTGC(4/8)
717 GCAGGT
BstBI 230 TTCGAA TT^CGAA
920 TTCGAA
BstF5I 49 CATCC GGATG(2/0)
843 GGATG
Cac8I 417 GCTGGC GC[ACGT]^[ACGT]GC
926 GCATGC
EaeI 259 TGGCCA [CT]^GGCC[GA]
362 TGGCCA
Fnu4HI 70 GCAGC GC^[ACGT]GC
708 GCTGC
FokI 49 CATCC GGATG(9/13)
843 GGATG
HaeIII 260 GGCC GG^CC
363 GGCC
ItaI 70 GCAGC GC^[ACGT]GC
708 GCTGC
MluNI 259 TGGCCA TGG^CCA
362 TGGCCA
MscI 259 TGGCCA TGG^CCA
362 TGGCCA
MslI 49 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
174 CATCTATATG
MwoI 412 GCCGTGCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
708 GCTGCTGGTGC
NspV 230 TTCGAA TT^CGAA
920 TTCGAA
PmaCI 402 CACGTG CAC^GTG
834 CACGTG
PmlI 402 CACGTG CAC^GTG
834 CACGTG
RcaI 605 TCATGA T^CATGA
821 TCATGA
SfuI 230 TTCGAA TT^CGAA
920 TTCGAA
SmlI 517 CTCAAG C^T[CT][GA]AG
884 CTCGAG
TseI 70 GCAGC G^C[AT]GC
708 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AfaI AluI BsaJI DdeI DpnII
HinfI MaeII MaeIII MboI MboII
MnlI MseI NdeII NlaIII PleI
RsaI Sau3AI TaqI TfiI Tru9I
Tsp509I TspRI TthHB8I