Restriction map for
pF1KB7144
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BcgI BcnI BfaI BfrI
BglI BglII BlnI BpmI BsaAI
BsaHI BsaI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspMI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HphI KasI KpnI
Ksp632I KspI MaeI MfeI MluI
MluNI MscI MspA1I MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 266 TCCGGA T^CCGGA
AcsI 91 AAATTT [GA]^AATT[CT]
AflIII 347 ACATGT A^C[GA][CT]GT
AluI 608 AGCT AG^CT
ApoI 91 AAATTT [GA]^AATT[CT]
BamHI 900 GGATCC G^GATCC
BanII 308 GAGCCC G[GA]GC[CT]^C
BciVI 556 GGATAC GTATCC(6/5)
BlpI 604 GCTGAGC GC^T[ACGT]AGC
BmyI 308 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 604 GCTGAGC GC^T[ACGT]AGC
BsaBI 633 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 266 TCCGGA [AT]^CCGG[AT]
BseAI 266 TCCGGA T^CCGGA
BseRI 403 CTCCTC GAGGAG(10/8)
BsgI 158 GTGCAG GTGCAG(16/14)
BsmI 570 GAATGC GAATGC(1/-1)
Bsp1286I 308 GAGCCC G[AGT]GC[ACT]^C
BspEI 266 TCCGGA T^CCGGA
BspLU11I 347 ACATGT A^CATGT
BsrBI 972 CCGCTC CCGCTC(-3/-3)
BstYI 900 GGATCC [GA]^GATC[CT]
CelII 604 GCTGAGC GC^T[ACGT]AGC
Cfr13I 419 GGGCC G^G[ACGT]CC
HapII 267 CCGG C^CGG
HinfI 842 GAATC G^A[ACGT]TC
HpaII 267 CCGG C^CGG
MaeII 373 ACGT A^CGT
MamI 633 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 900 GGATCC [GA]^GATC[CT]
MroI 266 TCCGGA T^CCGGA
MspI 267 CCGG C^CGG
NlaIV 900 GGATCC GG[ACGT]^[ACGT]CC
NspI 347 ACATGT [GA]CATG^[CT]
PflMI 422 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 746 CTGCAG CTGCA^G
Sau96I 419 GGGCC G^G[ACGT]CC
SfcI 746 CTGCAG C^T[GA][CT]AG
TfiI 842 GAATC G^A[AT]TC
Van91I 422 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 900 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 423 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
599 CAGTTGCTG
BclI 218 TGATCA T^GATCA
632 TGATCA
BsiYI 225 CCTCTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
422 CCAGAGACTGG
BslI 225 CCTCTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
422 CCAGAGACTGG
BsmFI 481 GGGAC GGGAC(10/14)
830 GTCCC
BspHI 215 TCATGA T^CATGA
983 TCATGA
BssKI 139 CCTGG ^CC[ACGT]GG
653 CCTGG
BstNI 139 CCTGG CC^[AT]GG
653 CCTGG
DsaI 800 CCATGG C^C[GA][CT]GG
954 CCATGG
EcoRII 139 CCTGG ^CC[AT]GG
653 CCTGG
FbaI 218 TGATCA T^GATCA
632 TGATCA
HaeIII 296 GGCC GG^CC
420 GGCC
MseI 523 TTAA T^TAA
782 TTAA
MslI 81 CACTAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
210 CATTTTCATG
MvaI 139 CCTGG CC^[AT]GG
653 CCTGG
NcoI 800 CCATGG C^CATGG
954 CCATGG
RcaI 215 TCATGA T^CATGA
983 TCATGA
ScrFI 139 CCTGG CC^[ACGT]GG
653 CCTGG
SfaNI 377 GCATC GCATC(5/9)
452 GCATC
SmlI 406 CTCAAG C^T[CT][GA]AG
672 CTTGAG
Tru9I 523 TTAA T^TAA
782 TTAA
Tsp509I 92 AATT ^AATT
906 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AfaI AlwI BbsI BbvI
BpuAI BsaJI BsmAI BsrI BstF5I
Cac8I DdeI DpnII EcoT14I FauI
Fnu4HI FokI ItaI MaeIII MboI
MboII MnlI MwoI NdeII NlaIII
RsaI Sau3AI StyI TseI Tsp45I
TspRI