Restriction map for
pF1KB7151
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsiEI BsiHKAI BsiWI
BsmBI BsmI BspDI BspHI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DdeI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoT22I
FseI FspI HaeII Hin1I HindIII
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 158 TCCGGA T^CCGGA
AlwNI 497 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 115 CTCGGG C^[CT]CG[GA]G
BalI 422 TGGCCA TGG^CCA
BclI 434 TGATCA T^GATCA
BcnI 924 CCGGG CC^[GC]GG
BseAI 158 TCCGGA T^CCGGA
BseRI 463 CTCCTC GAGGAG(10/8)
BsgI 525 CTGCAC GTGCAG(16/14)
BsmAI 4 GAGAC GTCTC(1/5)
BsoBI 115 CTCGGG C^[CT]CG[GA]G
Bsp1407I 824 TGTACA T^GTACA
BspEI 158 TCCGGA T^CCGGA
BspMI 45 ACCTGC ACCTGC(4/8)
BsrGI 824 TGTACA T^GTACA
BstYI 680 AGATCC [GA]^GATC[CT]
CfoI 632 GCGC GCG^C
EarI 67 CTCTTC CTCTTC(1/4)
EcoNI 969 CCTGACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 76 GATATC GAT^ATC
EcoT14I 257 CCATGG C^C[AT][AT]GG
FauI 949 CCCGC CCCGC(4/6)
FbaI 434 TGATCA T^GATCA
HgaI 377 GCGTC GACGC(5/10)
HhaI 632 GCGC GCG^C
HinP1I 632 GCGC G^CGC
HincII 591 GTTGAC GT[CT]^[GA]AC
HindII 591 GTTGAC GT[CT]^[GA]AC
Ksp632I 67 CTCTTC CTCTTC(1/4)
MflI 680 AGATCC [GA]^GATC[CT]
MluNI 422 TGGCCA TGG^CCA
MroI 158 TCCGGA T^CCGGA
MscI 422 TGGCCA TGG^CCA
MslI 483 CATTCGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 924 CCGGG CC^[GC]GG
NcoI 257 CCATGG C^CATGG
NspI 821 GCATGT [GA]CATG^[CT]
PleI 979 GACTC GAGTC(4/5)
PstI 1025 CTGCAG CTGCA^G
PvuII 879 CAGCTG CAG^CTG
SexAI 501 ACCTGGT A^CC[AT]GGT
SspBI 824 TGTACA T^GTACA
StyI 257 CCATGG C^C[AT][AT]GG
XhoII 680 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 9 AAATTC [GA]^AATT[CT]
295 AAATTC
AfaI 499 GTAC GT^AC
825 GTAC
AluI 880 AGCT AG^CT
1002 AGCT
AlwI 681 GATCC GGATC(4/5)
936 GATCC
ApaI 442 GGGCCC GGGCC^C
965 GGGCCC
ApoI 9 AAATTC [GA]^AATT[CT]
295 AAATTC
BanII 442 GGGCCC G[GA]GC[CT]^C
965 GGGCCC
BbsI 337 GTCTTC GAAGAC(2/6)
904 GTCTTC
BpmI 35 CTGGAG CTGGAG(16/14)
958 CTGGAG
BpuAI 337 GTCTTC GAAGAC(2/6)
904 GTCTTC
BsaWI 158 TCCGGA [AT]^CCGG[AT]
611 TCCGGT
BsrDI 354 CATTGC GCAATG(2/0)
470 CATTGC
Cac8I 58 GCTGGC GC[ACGT]^[ACGT]GC
523 GCCTGC
DsaI 203 CCGTGG C^C[GA][CT]GG
257 CCATGG
MseI 533 TTAA T^TAA
701 TTAA
MspA1I 366 CCGCTG C[AC]G^C[GT]G
879 CAGCTG
PpuMI 119 GGGTCCT [GA]G^G[AT]CC[CT]
722 GGGTCCT
RsaI 499 GTAC GT^AC
825 GTAC
SfaNI 384 GCATC GCATC(5/9)
605 GCATC
SfcI 494 CTACAG C^T[GA][CT]AG
1025 CTGCAG
TfiI 155 GATTC G^A[AT]TC
800 GAATC
Tru9I 533 TTAA T^TAA
701 TTAA
TspRI 845 CAGTG CA[GC]TG[ACGT][ACGT]^
993 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AvaII BbvI BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cfr13I DpnII
DraII EaeI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
TseI Tsp45I Tsp509I