################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MflI MluI MroI MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 256 GTCTAC GT^[AC][GT]AC AfaI 336 GTAC GT^AC AflIII 358 ACGTGT A^C[GA][CT]GT AspHI 1002 GAGCAC G[AT]GC[AT]^C BbrPI 357 CACGTG CAC^GTG BbsI 177 GAAGAC GAAGAC(2/6) BbvI 459 GCTGC GCAGC(8/12) BclI 626 TGATCA T^GATCA BmyI 1002 GAGCAC G[AGT]GC[ACT]^C BpmI 940 CTCCAG CTGGAG(16/14) BpuAI 177 GAAGAC GAAGAC(2/6) BsgI 1055 CTGCAC GTGCAG(16/14) BsiHKAI 1002 GAGCAC G[AT]GC[AT]^C BsmAI 362 GTCTC GTCTC(1/5) Bsp1286I 1002 GAGCAC G[AGT]GC[ACT]^C BspDI 374 ATCGAT AT^CGAT BspHI 86 TCATGA T^CATGA BspMI 442 ACCTGC ACCTGC(4/8) ClaI 374 ATCGAT AT^CGAT DraI 925 TTTAAA TTT^AAA DraII 659 AGGCCCT [GA]G^G[ACGT]CC[CT] EarI 12 CTCTTC CTCTTC(1/4) EcoNI 663 CCTAAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 659 AGGCCCT [GA]G^G[ACGT]CC[CT] FauI 407 CCCGC CCCGC(4/6) FbaI 626 TGATCA T^GATCA Fnu4HI 459 GCTGC GC^[ACGT]GC ItaI 459 GCTGC GC^[ACGT]GC Ksp632I 12 CTCTTC CTCTTC(1/4) MspA1I 276 CCGCTG C[AC]G^C[GT]G PleI 553 GAGTC GAGTC(4/5) PmaCI 357 CACGTG CAC^GTG PmlI 357 CACGTG CAC^GTG RcaI 86 TCATGA T^CATGA RsaI 336 GTAC GT^AC SfcI 819 CTGTAG C^T[GA][CT]AG TseI 459 GCTGC G^C[AT]GC XcmI 593 CCAAAAATATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### BalI 185 TGGCCA TGG^CCA 473 TGGCCA BfaI 341 CTAG C^TAG 802 CTAG BsaAI 357 CACGTG [CT]AC^GT[GA] 812 TACGTG BsiYI 55 CCCAAAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 663 CCTAAAGAAGG BslI 55 CCCAAAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 663 CCTAAAGAAGG BssKI 381 CCTGG ^CC[ACGT]GG 755 CCTGG BstF5I 1040 CATCC GGATG(2/0) 786 GGATG BstNI 381 CCTGG CC^[AT]GG 755 CCTGG DdeI 364 CTCAG C^T[ACGT]AG 1023 CTCAG DpnII 373 GATC ^GATC 627 GATC EcoRII 381 CCTGG ^CC[AT]GG 755 CCTGG FokI 1040 CATCC GGATG(9/13) 786 GGATG HgaI 416 GACGC GACGC(5/10) 320 GCGTC MaeI 341 CTAG C^TAG 802 CTAG MaeII 358 ACGT A^CGT 813 ACGT MaeIII 439 GTCAC ^GT[ACGT]AC 815 GTGAC MboI 373 GATC ^GATC 627 GATC MfeI 731 CAATTG C^AATTG 882 CAATTG MluNI 185 TGGCCA TGG^CCA 473 TGGCCA MscI 185 TGGCCA TGG^CCA 473 TGGCCA MunI 731 CAATTG C^AATTG 882 CAATTG MvaI 381 CCTGG CC^[AT]GG 755 CCTGG MwoI 410 GCCTTCGACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 466 GCAGGCTTGGC NdeII 373 GATC ^GATC 627 GATC Sau3AI 373 GATC ^GATC 627 GATC ScrFI 381 CCTGG CC^[ACGT]GG 755 CCTGG SfaNI 446 GCATC GCATC(5/9) 806 GCATC Tsp45I 439 GTCAC ^GT[GC]AC 815 GTGAC TspRI 189 CAGTG CA[GC]TG[ACGT][ACGT]^ 212 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AciI AcsI AluI ApoI BsmFI BsrI Cac8I Cfr13I EaeI HaeIII HinfI HphI MboII MnlI MseI MslI NlaIII Sau96I SspI TaqI TfiI Tru9I Tsp509I TthHB8I