Restriction map for
pF1KB7158
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# NO CUT ENZYMES ( 163 enzymes) #
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AccII AccIII AclI AcsI AflII
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI Asp700I
AspEI AspHI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaBI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmFI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DpnII DraI DraIII DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI HinP1I HincII
HindII HindIII HpaI KasI KspI
MamI MboI MfeI MflI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 547 GACGTC GACGT^C
Acc65I 425 GGTACC G^GTACC
AccI 90 GTCTAC GT^[AC][GT]AC
AcyI 547 GACGTC G[GA]^CG[CT]C
AfaI 426 GTAC GT^AC
AflIII 380 ACATGT A^C[GA][CT]GT
AgeI 968 ACCGGT A^CCGGT
AlwNI 773 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 859 ATTAAT AT^TAAT
AsnI 859 ATTAAT AT^TAAT
Asp718I 425 GGTACC G^GTACC
AspI 754 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 425 GGTACC G^G[CT][GA]CC
BsaHI 547 GACGTC G[GA]^CG[CT]C
BsaI 1075 GAGACC GGTCTC(1/5)
BsaWI 968 ACCGGT [AT]^CCGG[AT]
BsmBI 545 GAGACG CGTCTC(1/5)
BsmI 576 GAATGC GAATGC(1/-1)
BspLU11I 380 ACATGT A^CATGT
BspMI 406 ACCTGC ACCTGC(4/8)
BsrDI 524 CATTGC GCAATG(2/0)
BsrFI 968 ACCGGT [GA]^CCGG[CT]
BsrI 326 ACTGG ACTGG(1/-1)
Cfr10I 968 ACCGGT [GA]^CCGG[CT]
DraII 219 GGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 1077 GACCTTTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 287 CTCTTC CTCTTC(1/4)
EcoO109I 219 GGGTCCT [GA]G^G[ACGT]CC[CT]
Hin1I 547 GACGTC G[GA]^CG[CT]C
KpnI 425 GGTACC GGTAC^C
Ksp632I 287 CTCTTC CTCTTC(1/4)
MaeII 548 ACGT A^CGT
PflFI 754 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 968 ACCGGT A^CCGGT
PleI 88 GAGTC GAGTC(4/5)
PpuMI 219 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 859 ATTAAT AT^TAAT
RsaI 426 GTAC GT^AC
TaqI 543 TCGA T^CGA
TfiI 920 GATTC G^A[AT]TC
Tsp45I 752 GTGAC ^GT[GC]AC
Tth111I 754 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 543 TCGA T^CGA
XcmI 615 CCAATGGTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AciI 987 CCGC CCGC(-3/-1)
47 GCGG
AvaII 220 GGTCC G^G[AT]CC
337 GGACC
BbsI 717 GAAGAC GAAGAC(2/6)
50 GTCTTC
BfaI 29 CTAG C^TAG
236 CTAG
BpuAI 717 GAAGAC GAAGAC(2/6)
50 GTCTTC
BstXI 908 CCATCCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1006 CCAATTACTTGG
Cfr13I 220 GGTCC G^G[ACGT]CC
337 GGACC
EcoT14I 651 CCTTGG C^C[AT][AT]GG
1020 CCAAGG
HapII 969 CCGG C^CGG
1047 CCGG
HinfI 88 GAGTC G^A[ACGT]TC
920 GATTC
HpaII 969 CCGG C^CGG
1047 CCGG
HphI 404 TCACC GGTGA(8/7)
923 TCACC
MaeI 29 CTAG C^TAG
236 CTAG
MaeIII 196 GTTAC ^GT[ACGT]AC
752 GTGAC
MslI 127 CATTTAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
405 CACCTGCATG
MspI 969 CCGG C^CGG
1047 CCGG
MwoI 625 GCTGGGATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1046 GCCGGAAAAGC
NspI 380 ACATGT [GA]CATG^[CT]
685 ACATGC
Sau96I 220 GGTCC G^G[ACGT]CC
337 GGACC
SspI 184 AATATT AAT^ATT
672 AATATT
StyI 651 CCTTGG C^C[AT][AT]GG
1020 CCAAGG
TspRI 200 CACTG CA[GC]TG[ACGT][ACGT]^
990 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BsmAI BssKI BstF5I BstNI Cac8I
DdeI EcoRII Fnu4HI FokI HaeIII
ItaI MboII MnlI MseI MspA1I
MvaI NlaIII NlaIV PvuII ScrFI
SfaNI Tru9I TseI Tsp509I