Restriction map for
pF1KB7166
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BanI BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MluNI MscI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
Tth111I Van91I XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 92 TCCGGA T^CCGGA
AciI 1017 GCGG CCGC(-3/-1)
AcsI 148 AAATTT [GA]^AATT[CT]
AlwNI 538 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 148 AAATTT [GA]^AATT[CT]
AspHI 307 GAGCAC G[AT]GC[AT]^C
AvaI 1094 CCCGGG C^[CT]CG[GA]G
BciVI 420 GTATCC GTATCC(6/5)
BglII 65 AGATCT A^GATCT
BpmI 1003 CTGGAG CTGGAG(16/14)
BsaWI 92 TCCGGA [AT]^CCGG[AT]
BseAI 92 TCCGGA T^CCGGA
BsgI 624 GTGCAG GTGCAG(16/14)
BsiHKAI 307 GAGCAC G[AT]GC[AT]^C
BsmAI 776 GAGAC GTCTC(1/5)
BsoBI 1094 CCCGGG C^[CT]CG[GA]G
BspEI 92 TCCGGA T^CCGGA
BspHI 518 TCATGA T^CATGA
BstXI 50 CCACATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 357 GCGC GCG^C
DrdI 632 GACTTCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 489 TGGCCG [CT]^GGCC[GA]
EarI 403 GAAGAG CTCTTC(1/4)
EcoNI 24 CCTGCTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 1017 GCGGG CCCGC(4/6)
HhaI 357 GCGC GCG^C
HinP1I 357 GCGC G^CGC
Ksp632I 403 GAAGAG CTCTTC(1/4)
MroI 92 TCCGGA T^CCGGA
MseI 734 TTAA T^TAA
PshAI 1173 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 518 TCATGA T^CATGA
SmaI 1094 CCCGGG CCC^GGG
SmlI 805 CTTGAG C^T[CT][GA]AG
TaqI 774 TCGA T^CGA
Tru9I 734 TTAA T^TAA
Tsp45I 1070 GTCAC ^GT[GC]AC
TthHB8I 774 TCGA T^CGA
XbaI 667 TCTAGA T^CTAGA
XmaCI 1094 CCCGGG C^CCGGG
XmaI 1094 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BamHI 408 GGATCC G^GATCC
810 GGATCC
BanII 374 GGGCTC G[GA]GC[CT]^C
514 GGGCTC
BbsI 1170 GAAGAC GAAGAC(2/6)
88 GTCTTC
BfaI 28 CTAG C^TAG
668 CTAG
BpuAI 1170 GAAGAC GAAGAC(2/6)
88 GTCTTC
BsmFI 766 GGGAC GGGAC(10/14)
1091 GTCCC
BsrDI 873 CATTGC GCAATG(2/0)
352 GCAATG
BsrI 897 CCAGT ACTGG(1/-1)
1148 CCAGT
HphI 313 GGTGA GGTGA(8/7)
1071 TCACC
MaeI 28 CTAG C^TAG
668 CTAG
MaeIII 712 GTTAC ^GT[ACGT]AC
1070 GTCAC
MslI 51 CACATTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
243 CATCCTGGTG
PleI 18 GACTC GAGTC(4/5)
778 GACTC
PstI 535 CTGCAG CTGCA^G
1012 CTGCAG
PvuII 107 CAGCTG CAG^CTG
995 CAGCTG
SfaNI 196 GATGC GCATC(5/9)
747 GATGC
StuI 717 AGGCCT AGG^CCT
1061 AGGCCT
Tsp509I 149 AATT ^AATT
826 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AfaI AluI AlwI AvaII BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MboI MboII
MflI MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfcI
TfiI TseI TspRI XhoII