Restriction map for
pF1KB7168
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BcnI BfrI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstPI BstUI
BstXI Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII KasI KpnI
KspI MaeII MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tsp45I Tth111I
TthHB8I XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
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AccI 820 GTATAC GT^[AC][GT]AC
BclI 248 TGATCA T^GATCA
BglI 619 GCCATTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 408 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 991 GCATTC GAATGC(1/-1)
BspHI 707 TCATGA T^CATGA
BssSI 1090 CTCGTG CACGAG(-5/-1)
Bst1107I 820 GTATAC GTA^TAC
BstZ17I 820 GTATAC GTA^TAC
Cac8I 1165 GCTGGC GC[ACGT]^[ACGT]GC
DraII 467 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 585 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 212 CCATGG C^C[GA][CT]GG
EarI 502 CTCTTC CTCTTC(1/4)
EcoO109I 467 AGGCCCC [GA]G^G[ACGT]CC[CT]
FbaI 248 TGATCA T^GATCA
HaeIII 468 GGCC GG^CC
HincII 661 GTTGAC GT[CT]^[GA]AC
HindII 661 GTTGAC GT[CT]^[GA]AC
Ksp632I 502 CTCTTC CTCTTC(1/4)
MaeIII 455 GTTAC ^GT[ACGT]AC
MamI 408 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 212 CCATGG C^CATGG
NspI 848 GCATGT [GA]CATG^[CT]
PstI 986 CTGCAG CTGCA^G
RcaI 707 TCATGA T^CATGA
XbaI 399 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 19 enzymes) #
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AfaI 771 GTAC GT^AC
1048 GTAC
AvaII 478 GGACC G^G[AT]CC
581 GGACC
BsaJI 212 CCATGG C^C[ACGT][ACGT]GG
1013 CCTTGG
BsgI 857 CTGCAC GTGCAG(16/14)
1096 CTGCAC
BsmFI 580 GGGAC GGGAC(10/14)
883 GGGAC
BstYI 328 GGATCT [GA]^GATC[CT]
826 AGATCC
EcoT14I 212 CCATGG C^C[AT][AT]GG
1013 CCTTGG
HinfI 500 GACTC G^A[ACGT]TC
1222 GACTC
HphI 42 GGTGA GGTGA(8/7)
69 GGTGA
MflI 328 GGATCT [GA]^GATC[CT]
826 AGATCC
MwoI 619 GCCATTTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1086 GCTTCTCGTGC
PflMI 584 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
934 CCATTAGATGG
PleI 500 GACTC GAGTC(4/5)
1222 GACTC
RsaI 771 GTAC GT^AC
1048 GTAC
SfaNI 686 GCATC GCATC(5/9)
1112 GCATC
StyI 212 CCATGG C^C[AT][AT]GG
1013 CCTTGG
Van91I 584 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
934 CCATTAGATGG
XcmI 56 CCAACACAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
776 CCATGACTGTGATGG
XhoII 328 GGATCT [GA]^GATC[CT]
826 AGATCC
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbsI
BbvI BciVI BfaI BpmI BpuAI
BsiYI BslI BsrDI BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI ItaI MaeI
MboI MboII MnlI MseI MslI
MvaI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfcI Tru9I TseI
Tsp509I TspRI