Restriction map for
pF1KB7186
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
AscI AseI AsnI Asp718I AspEI
AvaII AviII AvrII BamHI BanI
BbeI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BsaBI BsaHI BseAI BsiWI BsmFI
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Cfr10I ClaI
CpoI DraI Eam1105I Eco47III EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
MaeI MaeIII MamI MfeI MluI
MroI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tru9I
Tsp45I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcsI 1117 AAATTC [GA]^AATT[CT]
AflIII 914 ACGTGT A^C[GA][CT]GT
Alw44I 1295 GTGCAC G^TGCAC
AocI 586 CCTAAGG CC^T[ACGT]AGG
ApaLI 1295 GTGCAC G^TGCAC
ApoI 1117 AAATTC [GA]^AATT[CT]
Asp700I 858 GAATCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 309 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 722 TGGCCA TGG^CCA
BbsI 813 GAAGAC GAAGAC(2/6)
BpuAI 813 GAAGAC GAAGAC(2/6)
BsgI 985 GTGCAG GTGCAG(16/14)
BsiEI 886 CGGCCG CG[GA][CT]^CG
BsmBI 452 GAGACG CGTCTC(1/5)
BsmI 538 GCATTC GAATGC(1/-1)
Bsp1407I 470 TGTACA T^GTACA
BsrBI 337 CCGCTC CCGCTC(-3/-3)
BsrGI 470 TGTACA T^GTACA
Bsu36I 586 CCTAAGG CC^T[ACGT]AGG
DraIII 1261 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 454 GACGTGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 886 CGGCCG C^GGCCG
EclXI 886 CGGCCG C^GGCCG
Eco52I 886 CGGCCG C^GGCCG
Eco81I 586 CCTAAGG CC^T[ACGT]AGG
KspI 385 CCGCGG CCGC^GG
MluNI 722 TGGCCA TGG^CCA
MscI 722 TGGCCA TGG^CCA
MslI 504 CACGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 467 GCATGT [GA]CATG^[CT]
PflFI 309 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 783 GAGCTC GAGCT^C
SacII 385 CCGCGG CCGC^GG
SexAI 560 ACCTGGT A^CC[AT]GGT
SmlI 183 CTTGAG C^T[CT][GA]AG
SspBI 470 TGTACA T^GTACA
Tth111I 309 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 858 GAATCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
ApaI 159 GGGCCC GGGCC^C
1303 GGGCCC
BbrPI 913 CACGTG CAC^GTG
1236 CACGTG
BsaAI 913 CACGTG [CT]AC^GT[GA]
1236 CACGTG
BsaI 314 GGTCTC GGTCTC(1/5)
564 GGTCTC
BsaWI 110 TCCGGT [AT]^CCGG[AT]
1036 ACCGGA
BseRI 331 GAGGAG GAGGAG(10/8)
1040 GAGGAG
BspMI 806 ACCTGC ACCTGC(4/8)
1160 ACCTGC
BssSI 616 CACGAG CACGAG(-5/-1)
730 CTCGTG
BstAPI 369 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
639 GCAGCAGCTGC
BstYI 360 GGATCT [GA]^GATC[CT]
1199 AGATCC
EaeI 722 TGGCCA [CT]^GGCC[GA]
886 CGGCCG
EarI 415 CTCTTC CTCTTC(1/4)
149 GAAGAG
HaeII 240 AGCGCC [GA]GCGC^[CT]
1051 AGCGCC
Ksp632I 415 CTCTTC CTCTTC(1/4)
149 GAAGAG
MflI 360 GGATCT [GA]^GATC[CT]
1199 AGATCC
NlaIII 212 CATG CATG^
468 CATG
PleI 600 GACTC GAGTC(4/5)
1250 GAGTC
PmaCI 913 CACGTG CAC^GTG
1236 CACGTG
PmlI 913 CACGTG CAC^GTG
1236 CACGTG
SapI 149 GAAGAGC GCTCTTC(1/4)
414 GCTCTTC
Tsp509I 928 AATT ^AATT
1118 AATT
XcmI 893 CCACGGTTATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
912 CCACGTGTTCCCTGG
XhoII 360 GGATCT [GA]^GATC[CT]
1199 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI BanII BbvI
BcnI BlpI BmyI BpmI Bpu1102I
BsaJI BsiHKAI BsiYI BslI BsmAI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIV PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TaqI TfiI TseI TspRI
TthHB8I XmaCI XmaI