Restriction map for pF1KB7189
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AviII       
AvrII       BalI        BamHI       BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaWI       BseAI       
BseRI       BsiHKAI     BsiWI       BsmBI       BsmFI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DdeI        DraI        DraIII      DrdI        Eam1105I    
EarI        Eco47III    Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HinfI       HpaI        
HphI        KpnI        Ksp632I     MaeI        MaeII       
MamI        MboII       MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SalI        SapI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        SspBI       SspI        StuI        
SwaI        TaqI        TfiI        Tru9I       Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AcyI          335  GGCGCC               G[GA]^CG[CT]C
AluI          152  AGCT                 AG^CT
AlwI          389  GGATC                GGATC(4/5)
AlwNI         100  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          214  GGGCCC               GGGCC^C
AvaII         209  GGTCC                G^G[AT]CC
BanI          335  GGCGCC               G^G[CT][GA]CC
BanII         214  GGGCCC               G[GA]GC[CT]^C
BbeI          335  GGCGCC               GGCGC^C
BglI          296  GCCGCGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          214  GGGCCC               G[AGT]GC[ACT]^C
BpmI          226  CTCCAG               CTGGAG(16/14)
BsaHI         335  GGCGCC               G[GA]^CG[CT]C
BsaI           21  GGTCTC               GGTCTC(1/5)
BsmAI          22  GTCTC                GTCTC(1/5)
Bsp1286I      214  GGGCCC               G[AGT]GC[ACT]^C
BsrFI         181  GCCGGC               [GA]^CCGG[CT]
Cfr10I        181  GCCGGC               [GA]^CCGG[CT]
DpnII         390  GATC                 ^GATC
DraII         214  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoNI         246  CCTCCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      214  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       287  CCATGG               C^C[AT][AT]GG
HgaI           39  GACGC                GACGC(5/10)
Hin1I         335  GGCGCC               G[GA]^CG[CT]C
KasI          335  GGCGCC               G^GCGCC
KspI          272  CCGCGG               CCGC^GG
MaeIII         37  GTGAC                ^GT[ACGT]AC
MboI          390  GATC                 ^GATC
NaeI          181  GCCGGC               GCC^GGC
NarI          335  GGCGCC               GG^CGCC
NcoI          287  CCATGG               C^CATGG
NdeII         390  GATC                 ^GATC
NgoMIV        181  GCCGGC               G^CCGGC
NlaIII        288  CATG                 CATG^
PshAI         112  GACTGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII         272  CCGCGG               CCGC^GG
Sau3AI        390  GATC                 ^GATC
SbfI          249  CCTGCAGG             CCTGCA^GG
SfoI          335  GGCGCC               GGC^GCC
SmaI          219  CCCGGG               CCC^GGG
Sse8387I      249  CCTGCAGG             CCTGCA^GG
StyI          287  CCATGG               C^C[AT][AT]GG
Tsp45I         37  GTGAC                ^GT[GC]AC
XmaCI         219  CCCGGG               C^CCGGG
XmaI          219  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AvaI          219  CCCGGG               C^[CT]CG[GA]G
              331  CTCGGG              
BsgI           95  CTGCAC               GTGCAG(16/14)
              157  CTGCAC              
BsiEI         179  CGGCCG               CG[GA][CT]^CG
              353  CGGCCG              
BsoBI         219  CCCGGG               C^[CT]CG[GA]G
              331  CTCGGG              
BstF5I        186  CATCC                GGATG(2/0)
              279  CATCC               
BstNI          26  CCTGG                CC^[AT]GG
              228  CCAGG               
DsaI          272  CCGCGG               C^C[GA][CT]GG
              287  CCATGG              
EaeI          179  CGGCCG               [CT]^GGCC[GA]
              353  CGGCCG              
EagI          179  CGGCCG               C^GGCCG
              353  CGGCCG              
EclXI         179  CGGCCG               C^GGCCG
              353  CGGCCG              
Eco52I        179  CGGCCG               C^GGCCG
              353  CGGCCG              
EcoRII         26  CCTGG                ^CC[AT]GG
              228  CCAGG               
FokI          186  CATCC                GGATG(9/13)
              279  CATCC               
MvaI           26  CCTGG                CC^[AT]GG
              228  CCAGG               
PstI          238  CTGCAG               CTGCA^G
              250  CTGCAG              
SfaNI         185  GCATC                GCATC(5/9)
              278  GCATC               
SfcI          238  CTGCAG               C^T[GA][CT]AG
              250  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   31 enzymes) #
#########################################

AccII       AciI        BbvI        BcnI        BsaJI       
BsiYI       BslI        BssKI       BstUI       Cac8I       
CfoI        Cfr13I      FauI        Fnu4HI      HaeII       
HaeIII      HapII       HhaI        HinP1I      HpaII       
ItaI        MnlI        MspA1I      MspI        MvnI        
MwoI        NciI        NlaIV       Sau96I      ScrFI       
TseI        
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