################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BseRI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI HphI KpnI Ksp632I MaeI MaeII MamI MboII MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SalI SapI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcyI 335 GGCGCC G[GA]^CG[CT]C AluI 152 AGCT AG^CT AlwI 389 GGATC GGATC(4/5) AlwNI 100 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 214 GGGCCC GGGCC^C AvaII 209 GGTCC G^G[AT]CC BanI 335 GGCGCC G^G[CT][GA]CC BanII 214 GGGCCC G[GA]GC[CT]^C BbeI 335 GGCGCC GGCGC^C BglI 296 GCCGCGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 214 GGGCCC G[AGT]GC[ACT]^C BpmI 226 CTCCAG CTGGAG(16/14) BsaHI 335 GGCGCC G[GA]^CG[CT]C BsaI 21 GGTCTC GGTCTC(1/5) BsmAI 22 GTCTC GTCTC(1/5) Bsp1286I 214 GGGCCC G[AGT]GC[ACT]^C BsrFI 181 GCCGGC [GA]^CCGG[CT] Cfr10I 181 GCCGGC [GA]^CCGG[CT] DpnII 390 GATC ^GATC DraII 214 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoNI 246 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 214 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoT14I 287 CCATGG C^C[AT][AT]GG HgaI 39 GACGC GACGC(5/10) Hin1I 335 GGCGCC G[GA]^CG[CT]C KasI 335 GGCGCC G^GCGCC KspI 272 CCGCGG CCGC^GG MaeIII 37 GTGAC ^GT[ACGT]AC MboI 390 GATC ^GATC NaeI 181 GCCGGC GCC^GGC NarI 335 GGCGCC GG^CGCC NcoI 287 CCATGG C^CATGG NdeII 390 GATC ^GATC NgoMIV 181 GCCGGC G^CCGGC NlaIII 288 CATG CATG^ PshAI 112 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 272 CCGCGG CCGC^GG Sau3AI 390 GATC ^GATC SbfI 249 CCTGCAGG CCTGCA^GG SfoI 335 GGCGCC GGC^GCC SmaI 219 CCCGGG CCC^GGG Sse8387I 249 CCTGCAGG CCTGCA^GG StyI 287 CCATGG C^C[AT][AT]GG Tsp45I 37 GTGAC ^GT[GC]AC XmaCI 219 CCCGGG C^CCGGG XmaI 219 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AvaI 219 CCCGGG C^[CT]CG[GA]G 331 CTCGGG BsgI 95 CTGCAC GTGCAG(16/14) 157 CTGCAC BsiEI 179 CGGCCG CG[GA][CT]^CG 353 CGGCCG BsoBI 219 CCCGGG C^[CT]CG[GA]G 331 CTCGGG BstF5I 186 CATCC GGATG(2/0) 279 CATCC BstNI 26 CCTGG CC^[AT]GG 228 CCAGG DsaI 272 CCGCGG C^C[GA][CT]GG 287 CCATGG EaeI 179 CGGCCG [CT]^GGCC[GA] 353 CGGCCG EagI 179 CGGCCG C^GGCCG 353 CGGCCG EclXI 179 CGGCCG C^GGCCG 353 CGGCCG Eco52I 179 CGGCCG C^GGCCG 353 CGGCCG EcoRII 26 CCTGG ^CC[AT]GG 228 CCAGG FokI 186 CATCC GGATG(9/13) 279 CATCC MvaI 26 CCTGG CC^[AT]GG 228 CCAGG PstI 238 CTGCAG CTGCA^G 250 CTGCAG SfaNI 185 GCATC GCATC(5/9) 278 GCATC SfcI 238 CTGCAG C^T[GA][CT]AG 250 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AccII AciI BbvI BcnI BsaJI BsiYI BslI BssKI BstUI Cac8I CfoI Cfr13I FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspA1I MspI MvnI MwoI NciI NlaIV Sau96I ScrFI TseI