################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BsaAI BsaBI BsaJI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstPI BstUI BstXI BstYI Bsu36I Cfr10I Cfr13I ClaI CpoI DpnII DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HincII HindII HindIII HpaI HphI KpnI KspI MaeI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI NaeI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 369 GTATAC GT^[AC][GT]AC AciI 578 GCGG CCGC(-3/-1) AcsI 60 AAATTT [GA]^AATT[CT] ApoI 60 AAATTT [GA]^AATT[CT] Asp700I 734 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbeI 799 GGCGCC GGCGC^C BlpI 21 GCTCAGC GC^T[ACGT]AGC BmyI 666 GGGCAC G[AGT]GC[ACT]^C BpmI 620 CTGGAG CTGGAG(16/14) Bpu1102I 21 GCTCAGC GC^T[ACGT]AGC BsaI 646 GAGACC GGTCTC(1/5) BsaWI 649 ACCGGA [AT]^CCGG[AT] BsmFI 860 GGGAC GGGAC(10/14) Bsp1286I 666 GGGCAC G[AGT]GC[ACT]^C BssKI 358 CCTGG ^CC[ACGT]GG BssSI 854 CTCGTG CACGAG(-5/-1) Bst1107I 369 GTATAC GTA^TAC BstNI 358 CCTGG CC^[AT]GG BstZ17I 369 GTATAC GTA^TAC Cac8I 141 GCATGC GC[ACGT]^[ACGT]GC CelII 21 GCTCAGC GC^T[ACGT]AGC CfoI 800 GCGC GCG^C EcoNI 358 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 358 CCTGG ^CC[AT]GG HaeII 799 GGCGCC [GA]GCGC^[CT] HapII 650 CCGG C^CGG HgaI 349 GACGC GACGC(5/10) HhaI 800 GCGC GCG^C HinP1I 800 GCGC G^CGC HpaII 650 CCGG C^CGG KasI 799 GGCGCC G^GCGCC MspA1I 576 CAGCGG C[AC]G^C[GT]G MspI 650 CCGG C^CGG MvaI 358 CCTGG CC^[AT]GG NarI 799 GGCGCC GG^CGCC NlaIII 142 CATG CATG^ NspI 141 GCATGC [GA]CATG^[CT] ScrFI 358 CCTGG CC^[ACGT]GG SfaNI 66 GCATC GCATC(5/9) SfcI 729 CTATAG C^T[GA][CT]AG SfoI 799 GGCGCC GGC^GCC SphI 141 GCATGC GCATG^C XmnI 734 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AcyI 349 GACGCC G[GA]^CG[CT]C 799 GGCGCC BsaHI 349 GACGCC G[GA]^CG[CT]C 799 GGCGCC BstF5I 197 CATCC GGATG(2/0) 507 GGATG DraI 162 TTTAAA TTT^AAA 898 TTTAAA FokI 197 CATCC GGATG(9/13) 507 GGATG HaeIII 39 GGCC GG^CC 119 GGCC Hin1I 349 GACGCC G[GA]^CG[CT]C 799 GGCGCC MaeIII 191 GTGAC ^GT[ACGT]AC 616 GTCAC PflMI 691 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 745 CCACCTTTTGG TfiI 88 GATTC G^A[AT]TC 277 GAATC Tsp45I 191 GTGAC ^GT[GC]AC 616 GTCAC TspRI 332 CACTG CA[GC]TG[ACGT][ACGT]^ 618 CACTG Van91I 691 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 745 CCACCTTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AfaI AluI BanI BbsI BbvI BpuAI BseRI BsiYI BslI BsmAI BsrI DdeI EarI Fnu4HI HinfI ItaI Ksp632I MboII MnlI MseI MwoI NlaIV PleI RsaI TaqI Tru9I TseI Tsp509I TthHB8I