################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII AccI AccII AccIII AclI AflII AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbvCI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaI BseAI BsiEI BsiWI BsmI BspDI BspEI BspLU11I BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FspI HindIII HpaI KspI MaeI MaeIII MamI MfeI MluI MroI MseI MunI MvnI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StyI SwaI TfiI Tru9I Tsp45I Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 260 GGTACC G^GTACC AcsI 1234 GAATTT [GA]^AATT[CT] AflIII 296 ACGTGT A^C[GA][CT]GT AgeI 302 ACCGGT A^CCGGT ApoI 1234 GAATTT [GA]^AATT[CT] Asp718I 260 GGTACC G^GTACC BamHI 156 GGATCC G^GATCC BbeI 7 GGCGCC GGCGC^C BbrPI 295 CACGTG CAC^GTG BbsI 580 GTCTTC GAAGAC(2/6) BcgI 127 CGACGCCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 994 GTATCC GTATCC(6/5) BpuAI 580 GTCTTC GAAGAC(2/6) BsmAI 100 GAGAC GTCTC(1/5) BsmBI 100 GAGACG CGTCTC(1/5) BspHI 1086 TCATGA T^CATGA BsrDI 786 GCAATG GCAATG(2/0) BstXI 636 CCAGTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 1129 GGGACCC [GA]G^G[ACGT]CC[CT] DsaI 1340 CCACGG C^C[GA][CT]GG EarI 1242 GAAGAG CTCTTC(1/4) EcoO109I 1129 GGGACCC [GA]G^G[ACGT]CC[CT] FseI 60 GGCCGGCC GGCCGG^CC HincII 1213 GTCAAC GT[CT]^[GA]AC HindII 1213 GTCAAC GT[CT]^[GA]AC KasI 7 GGCGCC G^GCGCC KpnI 260 GGTACC GGTAC^C Ksp632I 1242 GAAGAG CTCTTC(1/4) MslI 948 CATTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 7 GGCGCC GG^CGCC NspI 1355 GCATGT [GA]CATG^[CT] PinAI 302 ACCGGT A^CCGGT PmaCI 295 CACGTG CAC^GTG PmlI 295 CACGTG CAC^GTG PpuMI 1129 GGGACCC [GA]G^G[AT]CC[CT] RcaI 1086 TCATGA T^CATGA SacI 1159 GAGCTC GAGCT^C SapI 1242 GAAGAGC GCTCTTC(1/4) ScaI 701 AGTACT AGT^ACT SfoI 7 GGCGCC GGC^GCC SmaI 1124 CCCGGG CCC^GGG TaqI 197 TCGA T^CGA TthHB8I 197 TCGA T^CGA XmaCI 1124 CCCGGG C^CCGGG XmaI 1124 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AcyI 7 GGCGCC G[GA]^CG[CT]C 128 GACGCC AlwNI 418 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 1180 CAGGGGCTG Aor51HI 140 AGCGCT AGC^GCT 1253 AGCGCT AvaI 43 CTCGGG C^[CT]CG[GA]G 1124 CCCGGG BalI 214 TGGCCA TGG^CCA 1193 TGGCCA BanII 646 GGGCTC G[GA]GC[CT]^C 1159 GAGCTC BpmI 345 CTCCAG CTGGAG(16/14) 357 CTGGAG BsaAI 295 CACGTG [CT]AC^GT[GA] 805 TACGTG BsaHI 7 GGCGCC G[GA]^CG[CT]C 128 GACGCC BsaWI 188 ACCGGA [AT]^CCGG[AT] 302 ACCGGT BsoBI 43 CTCGGG C^[CT]CG[GA]G 1124 CCCGGG Bsp1407I 752 TGTACA T^GTACA 1358 TGTACA BspMI 263 ACCTGC ACCTGC(4/8) 512 ACCTGC BsrGI 752 TGTACA T^GTACA 1358 TGTACA BstYI 156 GGATCC [GA]^GATC[CT] 791 GGATCT Eco47III 140 AGCGCT AGC^GCT 1253 AGCGCT EcoNI 147 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 867 CCTCTATGAGG HgaI 128 GACGC GACGC(5/10) 592 GACGC Hin1I 7 GGCGCC G[GA]^CG[CT]C 128 GACGCC HphI 954 GGTGA GGTGA(8/7) 1169 TCACC MaeII 296 ACGT A^CGT 806 ACGT MflI 156 GGATCC [GA]^GATC[CT] 791 GGATCT MluNI 214 TGGCCA TGG^CCA 1193 TGGCCA MscI 214 TGGCCA TGG^CCA 1193 TGGCCA NaeI 4 GCCGGC GCC^GGC 61 GCCGGC NgoMIV 4 GCCGGC G^CCGGC 61 GCCGGC PstI 331 CTGCAG CTGCA^G 1383 CTGCAG SfaNI 553 GCATC GCATC(5/9) 986 GCATC SfcI 331 CTGCAG C^T[GA][CT]AG 1383 CTGCAG SmlI 1264 CTTGAG C^T[CT][GA]AG 1306 CTCAAG SspBI 752 TGTACA T^GTACA 1358 TGTACA StuI 321 AGGCCT AGG^CCT 1376 AGGCCT Tsp509I 718 AATT ^AATT 1235 AATT XhoII 156 GGATCC [GA]^GATC[CT] 791 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII BanI BbvI BcnI BmyI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EaeI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI TseI TspRI