Restriction map for
pF1KB7231
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII AccI AccIII AflII AgeI
AlwNI AocI AscI AseI AsnI
Asp700I AviII AvrII BamHI BbvCI
BcgI BciVI BclI BfaI BfrI
BlnI BsaBI BsaWI BseAI BsgI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DrdI EarI Eco81I EcoNI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI Ksp632I
KspI MaeI MamI MfeI MluI
MroI MseI MunI NaeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
PvuI RcaI RsrII SacII SalI
SapI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI SspBI SspI
SwaI TfiI Tru9I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 442 GGTACC G^GTACC
AclI 1158 AACGTT AA^CGTT
AcsI 1216 GAATTC [GA]^AATT[CT]
AcyI 49 GGCGCC G[GA]^CG[CT]C
AflIII 176 ACGTGT A^C[GA][CT]GT
AhdI 1246 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 145 GTGCAC G^TGCAC
AlwI 198 GGATC GGATC(4/5)
Aor51HI 615 AGCGCT AGC^GCT
ApaI 235 GGGCCC GGGCC^C
ApaLI 145 GTGCAC G^TGCAC
ApoI 1216 GAATTC [GA]^AATT[CT]
Asp718I 442 GGTACC G^GTACC
AspEI 1246 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 231 CTCGGG C^[CT]CG[GA]G
BalI 758 TGGCCA TGG^CCA
BbeI 49 GGCGCC GGCGC^C
BbrPI 711 CACGTG CAC^GTG
BbsI 250 GTCTTC GAAGAC(2/6)
BglI 237 GCCCTTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 461 AGATCT A^GATCT
BlpI 859 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 859 GCTGAGC GC^T[ACGT]AGC
BpuAI 250 GTCTTC GAAGAC(2/6)
BsaHI 49 GGCGCC G[GA]^CG[CT]C
BsmI 738 GAATGC GAATGC(1/-1)
BsoBI 231 CTCGGG C^[CT]CG[GA]G
BstXI 761 CCATGAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 859 GCTGAGC GC^T[ACGT]AGC
Eam1105I 1246 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 615 AGCGCT AGC^GCT
EcoRI 1216 GAATTC G^AATTC
HgaI 311 GACGC GACGC(5/10)
Hin1I 49 GGCGCC G[GA]^CG[CT]C
KasI 49 GGCGCC G^GCGCC
KpnI 442 GGTACC GGTAC^C
MluNI 758 TGGCCA TGG^CCA
MscI 758 TGGCCA TGG^CCA
NarI 49 GGCGCC GG^CGCC
NcoI 215 CCATGG C^CATGG
NdeI 627 CATATG CA^TATG
PmaCI 711 CACGTG CAC^GTG
PmlI 711 CACGTG CAC^GTG
PpuMI 637 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 910 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1158 AACGTT AA^CGTT
SacI 862 GAGCTC GAGCT^C
SbfI 387 CCTGCAGG CCTGCA^GG
SexAI 680 ACCTGGT A^CC[AT]GGT
SfoI 49 GGCGCC GGC^GCC
SmlI 1129 CTCAAG C^T[CT][GA]AG
SphI 959 GCATGC GCATG^C
Sse8387I 387 CCTGCAGG CCTGCA^GG
StuI 1172 AGGCCT AGG^CCT
TaqI 89 TCGA T^CGA
Tsp509I 1217 AATT ^AATT
TthHB8I 89 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 184 CGCG CG^CG
327 CGCG
AvaII 638 GGACC G^G[AT]CC
771 GGTCC
BpmI 130 CTGGAG CTGGAG(16/14)
202 CTGGAG
BsaAI 175 TACGTG [CT]AC^GT[GA]
711 CACGTG
BsaI 592 GAGACC GGTCTC(1/5)
1137 GGTCTC
BsiEI 921 CGGCCG CG[GA][CT]^CG
1283 CGGCCG
BsmAI 592 GAGAC GTCTC(1/5)
1138 GTCTC
BssSI 154 CACGAG CACGAG(-5/-1)
193 CTCGTG
BstEII 667 GGTGACC G^GT[ACGT]ACC
705 GGTCACC
BstPI 667 GGTGACC G^GT[ACGT]ACC
705 GGTCACC
BstUI 184 CGCG CG^CG
327 CGCG
BstYI 198 GGATCT [GA]^GATC[CT]
461 AGATCT
DraIII 679 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
708 CACCACGTG
EagI 921 CGGCCG C^GGCCG
1283 CGGCCG
EclXI 921 CGGCCG C^GGCCG
1283 CGGCCG
Eco52I 921 CGGCCG C^GGCCG
1283 CGGCCG
EcoO65I 667 GGTGACC G^GT[ACGT]ACC
705 GGTCACC
HaeII 49 GGCGCC [GA]GCGC^[CT]
615 AGCGCT
MboII 251 TCTTC GAAGA(8/7)
794 TCTTC
MflI 198 GGATCT [GA]^GATC[CT]
461 AGATCT
MvnI 184 CGCG CG^CG
327 CGCG
NspI 81 ACATGC [GA]CATG^[CT]
959 GCATGC
PflMI 53 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
408 CCACTCCCTGG
PstI 124 CTGCAG CTGCA^G
388 CTGCAG
SfcI 124 CTGCAG C^T[GA][CT]AG
388 CTGCAG
Van91I 53 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
408 CCACTCCCTGG
XcmI 408 CCACTCCCTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
965 CCATGAGGGAGGTGG
XhoII 198 GGATCT [GA]^GATC[CT]
461 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AspHI AspI
BanI BanII BbvI BcnI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflFI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TseI Tsp45I TspRI Tth111I