Restriction map for
pF1KB7233
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AclI AcsI AcyI
AflII AflIII Alw44I AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsiEI BsiWI
BsmBI BspHI BspLU11I BspMI BsrDI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII CpoI
DraI DrdI EagI EclXI Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MseI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII ScaI SfiI SfoI
SfuI SmaI SnaBI SpeI SphI
SspI StuI SwaI Tru9I Tth111I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 174 GTCGAC GT^[AC][GT]AC
AccIII 770 TCCGGA T^CCGGA
AgeI 1306 ACCGGT A^CCGGT
AhdI 448 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 587 AGCGCT AGC^GCT
AspEI 448 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 478 GAGCTC G[AT]GC[AT]^C
AvaI 409 CTCGAG C^[CT]CG[GA]G
BanI 92 GGTGCC G^G[CT][GA]CC
BglI 139 GCCAACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 770 TCCGGA T^CCGGA
BsgI 498 GTGCAG GTGCAG(16/14)
BsiHKAI 478 GAGCTC G[AT]GC[AT]^C
BsoBI 409 CTCGAG C^[CT]CG[GA]G
Bsp1407I 528 TGTACA T^GTACA
BspDI 82 ATCGAT AT^CGAT
BspEI 770 TCCGGA T^CCGGA
BsrBI 1141 GAGCGG CCGCTC(-3/-3)
BsrFI 1306 ACCGGT [GA]^CCGG[CT]
BsrGI 528 TGTACA T^GTACA
BstXI 347 CCAACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1306 ACCGGT [GA]^CCGG[CT]
ClaI 82 ATCGAT AT^CGAT
DraIII 435 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 448 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1108 GAAGAG CTCTTC(1/4)
Eco47III 587 AGCGCT AGC^GCT
HincII 174 GTCGAC GT[CT]^[GA]AC
HindII 174 GTCGAC GT[CT]^[GA]AC
Ksp632I 1108 GAAGAG CTCTTC(1/4)
MroI 770 TCCGGA T^CCGGA
MslI 862 CACCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 409 CTCGAG C^TCGAG
PinAI 1306 ACCGGT A^CCGGT
PpuMI 241 GGGACCT [GA]G^G[AT]CC[CT]
SacI 478 GAGCTC GAGCT^C
SalI 174 GTCGAC G^TCGAC
SapI 1108 GAAGAGC GCTCTTC(1/4)
SbfI 909 CCTGCAGG CCTGCA^GG
SexAI 1160 ACCAGGT A^CC[AT]GGT
SgrAI 1305 CACCGGTG C[GA]^CCGG[CT]G
Sse8387I 909 CCTGCAGG CCTGCA^GG
SspBI 528 TGTACA T^GTACA
Tsp509I 534 AATT ^AATT
XhoI 409 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 543 CGCG CG^CG
648 CGCG
AlwNI 195 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1162 CAGGTGCTG
AvaII 242 GGACC G^G[AT]CC
1056 GGACC
BssSI 427 CACGAG CACGAG(-5/-1)
724 CACGAG
BstF5I 642 GGATG GGATG(2/0)
867 GGATG
BstUI 543 CGCG CG^CG
648 CGCG
DraII 241 GGGACCT [GA]G^G[ACGT]CC[CT]
277 GGGGCCT
EcoO109I 241 GGGACCT [GA]G^G[ACGT]CC[CT]
277 GGGGCCT
FauI 623 GCGGG CCCGC(4/6)
828 GCGGG
FokI 642 GGATG GGATG(9/13)
867 GGATG
HaeII 587 AGCGCT [GA]GCGC^[CT]
998 AGCGCC
HgaI 627 GACGC GACGC(5/10)
646 GACGC
MaeII 143 ACGT A^CGT
1175 ACGT
MboII 489 GAAGA GAAGA(8/7)
1108 GAAGA
MvnI 543 CGCG CG^CG
648 CGCG
SmlI 409 CTCGAG C^T[CT][GA]AG
712 CTCAAG
TfiI 269 GAATC G^A[AT]TC
741 GATTC
XcmI 125 CCATGTGCCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
659 CCAAGGCCGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI BalI
BanII BbvI BcnI BmyI BpmI
BsaI BsaJI BsaWI BseRI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EcoNI EcoRII EcoT14I Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MflI MluNI MnlI MscI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XhoII