Restriction map for
pF1KB7235
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI AspEI AspI BalI BbeI
BbrPI BbsI BciVI BclI BfrI
BglI BpuAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI Hin1I
HincII HindII HpaI KasI MamI
MfeI MluI MluNI MroI MscI
MseI MunI NarI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI TaqI TfiI
Tru9I Tth111I TthHB8I Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 980 GGTACC G^GTACC
AflIII 272 ACGTGT A^C[GA][CT]GT
Asp718I 980 GGTACC G^GTACC
AviII 767 TGCGCA TGC^GCA
AvrII 1614 CCTAGG C^CTAGG
BanI 980 GGTACC G^G[CT][GA]CC
BcgI 268 CGATACGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1199 AGATCT A^GATCT
BlnI 1614 CCTAGG C^CTAGG
BlpI 634 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 634 GCTCAGC GC^T[ACGT]AGC
BsaAI 271 TACGTG [CT]AC^GT[GA]
BsmBI 304 CGTCTC CGTCTC(1/5)
BsrBI 1487 CCGCTC CCGCTC(-3/-3)
BsrFI 396 GCCGGC [GA]^CCGG[CT]
BssHII 1383 GCGCGC G^CGCGC
BssSI 1425 CTCGTG CACGAG(-5/-1)
BstAPI 1494 GCATCTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1237 CCATCGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 634 GCTCAGC GC^T[ACGT]AGC
Cfr10I 396 GCCGGC [GA]^CCGG[CT]
FspI 767 TGCGCA TGC^GCA
HgaI 303 GCGTC GACGC(5/10)
HindIII 689 AAGCTT A^AGCTT
KpnI 980 GGTACC GGTAC^C
KspI 208 CCGCGG CCGC^GG
MaeII 272 ACGT A^CGT
MslI 1089 CATCCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 396 GCCGGC GCC^GGC
NgoMIV 396 GCCGGC G^CCGGC
NheI 925 GCTAGC G^CTAGC
SacI 1422 GAGCTC GAGCT^C
SacII 208 CCGCGG CCGC^GG
SmaI 1113 CCCGGG CCC^GGG
Tsp509I 1220 AATT ^AATT
XmaCI 1113 CCCGGG C^CCGGG
XmaI 1113 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwNI 190 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
605 CAGCCTCTG
AocI 492 CCTGAGG CC^T[ACGT]AGG
1626 CCTTAGG
ApaI 11 GGGCCC GGGCC^C
1328 GGGCCC
Asp700I 114 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
336 GAATGTGTTC
AspHI 632 GTGCTC G[AT]GC[AT]^C
1422 GAGCTC
BamHI 808 GGATCC G^GATCC
1151 GGATCC
BbvCI 1509 CCTCAGC CCTCAGC(-5/-2)
1536 GCTGAGG
BsiHKAI 632 GTGCTC G[AT]GC[AT]^C
1422 GAGCTC
BsrDI 1860 CATTGC GCAATG(2/0)
867 GCAATG
BstF5I 1089 CATCC GGATG(2/0)
156 GGATG
Bsu36I 492 CCTGAGG CC^T[ACGT]AGG
1626 CCTTAGG
Eco81I 492 CCTGAGG CC^T[ACGT]AGG
1626 CCTTAGG
EcoNI 1092 CCTTGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1721 CCTCAGAGAGG
FokI 1089 CATCC GGATG(9/13)
156 GGATG
HaeII 171 AGCGCC [GA]GCGC^[CT]
1513 AGCGCC
NcoI 422 CCATGG C^CATGG
1826 CCATGG
NspI 248 GCATGC [GA]CATG^[CT]
1575 GCATGC
SphI 248 GCATGC GCATG^C
1575 GCATGC
StuI 390 AGGCCT AGG^CCT
595 AGGCCT
XmnI 114 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
336 GAATGTGTTC
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstEII BstNI BstPI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I TspRI XcmI XhoII