Restriction map for
pF1KB7261
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AclI AcsI AcyI
AfaI AflII AflIII AgeI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsiEI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BsrI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII Hin1I HindIII HpaI
KasI KpnI KspI MaeI MaeIII
MamI MfeI MluI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacII SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 328 GTCGAC GT^[AC][GT]AC
AccIII 555 TCCGGA T^CCGGA
AhdI 331 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 331 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 191 GAGCTC G[AT]GC[AT]^C
AvaI 223 CCCGAG C^[CT]CG[GA]G
BalI 455 TGGCCA TGG^CCA
BanII 191 GAGCTC G[GA]GC[CT]^C
BciVI 37 GGATAC GTATCC(6/5)
BcnI 123 CCGGG CC^[GC]GG
BsaWI 555 TCCGGA [AT]^CCGG[AT]
BseAI 555 TCCGGA T^CCGGA
BsgI 384 CTGCAC GTGCAG(16/14)
BsiHKAI 191 GAGCTC G[AT]GC[AT]^C
BsoBI 223 CCCGAG C^[CT]CG[GA]G
BspEI 555 TCCGGA T^CCGGA
BsrFI 388 ACCGGC [GA]^CCGG[CT]
BssSI 402 CACGAG CACGAG(-5/-1)
BstAPI 386 GCACCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 388 ACCGGC [GA]^CCGG[CT]
Eam1105I 331 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 328 GTCGAC GT[CT]^[GA]AC
HindII 328 GTCGAC GT[CT]^[GA]AC
MluNI 455 TGGCCA TGG^CCA
MroI 555 TCCGGA T^CCGGA
MscI 455 TGGCCA TGG^CCA
MseI 490 TTAA T^TAA
MslI 576 CACGGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 278 CCGCTG C[AC]G^C[GT]G
NciI 123 CCGGG CC^[GC]GG
NspI 216 GCATGT [GA]CATG^[CT]
PflMI 48 CCATGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 191 GAGCTC GAGCT^C
SalI 328 GTCGAC G^TCGAC
Tru9I 490 TTAA T^TAA
Tsp509I 488 AATT ^AATT
TspRI 408 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 48 CCATGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AluI 192 AGCT AG^CT
270 AGCT
AlwI 72 GATCC GGATC(4/5)
111 GATCC
BmyI 191 GAGCTC G[AGT]GC[ACT]^C
220 GTGCCC
BpmI 130 CTCCAG CTGGAG(16/14)
142 CTGGAG
BseRI 25 CTCCTC GAGGAG(10/8)
514 GAGGAG
BsmFI 125 GGGAC GGGAC(10/14)
274 GTCCC
Bsp1286I 191 GAGCTC G[AGT]GC[ACT]^C
220 GTGCCC
BstF5I 120 CATCC GGATG(2/0)
579 GGATG
BstYI 71 AGATCC [GA]^GATC[CT]
110 AGATCC
CfoI 530 GCGC GCG^C
536 GCGC
DdeI 187 CTGAG C^T[ACGT]AG
212 CTAAG
DraII 4 AGGACCT [GA]G^G[ACGT]CC[CT]
125 GGGACCT
EaeI 56 TGGCCG [CT]^GGCC[GA]
455 TGGCCA
EarI 252 GAAGAG CTCTTC(1/4)
568 GAAGAG
EcoO109I 4 AGGACCT [GA]G^G[ACGT]CC[CT]
125 GGGACCT
EcoT14I 8 CCTTGG C^C[AT][AT]GG
458 CCAAGG
FokI 120 CATCC GGATG(9/13)
579 GGATG
HgaI 431 GCGTC GACGC(5/10)
446 GCGTC
HhaI 530 GCGC GCG^C
536 GCGC
HinP1I 530 GCGC G^CGC
536 GCGC
Ksp632I 252 GAAGAG CTCTTC(1/4)
568 GAAGAG
MaeII 337 ACGT A^CGT
470 ACGT
MflI 71 AGATCC [GA]^GATC[CT]
110 AGATCC
PpuMI 4 AGGACCT [GA]G^G[AT]CC[CT]
125 GGGACCT
SfaNI 119 GCATC GCATC(5/9)
260 GCATC
StyI 8 CCTTGG C^C[AT][AT]GG
458 CCAAGG
XhoII 71 AGATCC [GA]^GATC[CT]
110 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AvaII BbvI BsaJI
BsiYI BslI BssKI BstNI BstUI
Cac8I Cfr13I DpnII EcoRII FauI
Fnu4HI HaeIII HapII HinfI HpaII
HphI ItaI MboI MboII MnlI
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI TaqI TfiI TseI TthHB8I