##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmAI       BsmBI       
BsoBI       BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        HpaII       
KasI        KpnI        Ksp632I     KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AclI          334  AACGTT               AA^CGTT
AfaI          562  GTAC                 GT^AC
AvaII        1023  GGTCC                G^G[AT]CC
BbsI         1225  GTCTTC               GAAGAC(2/6)
BpuAI        1225  GTCTTC               GAAGAC(2/6)
BsmI          308  GAATGC               GAATGC(1/-1)
Bsp1407I      561  TGTACA               T^GTACA
BsrGI         561  TGTACA               T^GTACA
BstAPI        303  GCACAGAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         193  CCAGATCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI          355  TTTAAA               TTT^AAA
DraIII        997  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          184  CCATGG               C^C[GA][CT]GG
EcoRI         824  GAATTC               G^AATTC
MaeII         335  ACGT                 A^CGT
MaeIII        801  GTCAC                ^GT[ACGT]AC
NcoI          184  CCATGG               C^CATGG
NspI         1137  ACATGC               [GA]CATG^[CT]
PpuMI        1022  AGGTCCT              [GA]G^G[AT]CC[CT]
Psp1406I      334  AACGTT               AA^CGTT
PstI          727  CTGCAG               CTGCA^G
RsaI          562  GTAC                 GT^AC
SfcI          727  CTGCAG               C^T[GA][CT]AG
SspBI         561  TGTACA               T^GTACA
TaqI          408  TCGA                 T^CGA
Tsp45I        801  GTCAC                ^GT[GC]AC
TthHB8I       408  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AciI           18  CCGC                 CCGC(-3/-1)
              718  GCGG                
AcsI          824  GAATTC               [GA]^AATT[CT]
             1164  GAATTT              
ApaI          189  GGGCCC               GGGCC^C
              589  GGGCCC              
ApoI          824  GAATTC               [GA]^AATT[CT]
             1164  GAATTT              
AspHI        1053  GTGCTC               G[AT]GC[AT]^C
             1144  GTGCTC              
BamHI         656  GGATCC               G^GATCC
             1090  GGATCC              
BanII         189  GGGCCC               G[GA]GC[CT]^C
              589  GGGCCC              
BfaI           61  CTAG                 C^TAG
              265  CTAG                
BsiHKAI      1053  GTGCTC               G[AT]GC[AT]^C
             1144  GTGCTC              
BspMI         147  ACCTGC               ACCTGC(4/8)
              725  ACCTGC              
BstF5I         15  CATCC                GGATG(2/0)
             1034  CATCC               
FokI           15  CATCC                GGATG(9/13)
             1034  CATCC               
MaeI           61  CTAG                 C^TAG
              265  CTAG                
MseI          356  TTAA                 T^TAA
              512  TTAA                
MwoI          303  GCACAGAATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              471  GCTTATTCGGC         
TfiI           57  GATTC                G^A[AT]TC
              213  GATTC               
Tru9I         356  TTAA                 T^TAA
              512  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AluI        AlwI        Asp700I     BbvI        BmyI        
BpmI        BsaJI       BseRI       BsiYI       BslI        
BsmFI       Bsp1286I    BsrI        BssKI       BstNI       
BstYI       Cfr13I      DdeI        DpnII       DraII       
EcoO109I    EcoRII      EcoT14I     Fnu4HI      HaeIII      
HindIII     HinfI       HphI        ItaI        MboI        
MboII       MflI        MnlI        MvaI        NdeII       
NlaIII      NlaIV       PleI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TseI        Tsp509I     
TspRI       XhoII       XmnI