Restriction map for pF1KB7271
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvrII       
BamHI       BbrPI       BbvCI       BcgI        BciVI       
BclI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BssHII      Bst1107I    
BstAPI      BstBI       BstZ17I     CelII       ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HgaI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        NcoI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SspI        StuI        SwaI        Tth111I     Van91I      
XbaI        XhoI        


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# ONE CUT ENZYMES (   65 enzymes) #
###################################

AccII         405  CGCG                 CG^CG
AcyI         1370  GGCGCC               G[GA]^CG[CT]C
AfaI          347  GTAC                 GT^AC
AflII        1180  CTTAAG               C^TTAAG
Asp700I      1212  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         1449  CCCGGG               C^[CT]CG[GA]G
AviII        1340  TGCGCA               TGC^GCA
BalI          383  TGGCCA               TGG^CCA
BanI         1370  GGCGCC               G^G[CT][GA]CC
BbeI         1370  GGCGCC               GGCGC^C
BbsI         1155  GAAGAC               GAAGAC(2/6)
BfrI         1180  CTTAAG               C^TTAAG
BglI          376  GCCGTAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI        1155  GAAGAC               GAAGAC(2/6)
BsaHI        1370  GGCGCC               G[GA]^CG[CT]C
BsaI          891  GAGACC               GGTCTC(1/5)
BsoBI        1449  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      346  TGTACA               T^GTACA
BsrBI         396  GAGCGG               CCGCTC(-3/-3)
BsrFI         533  GCCGGC               [GA]^CCGG[CT]
BsrGI         346  TGTACA               T^GTACA
BssSI         289  CTCGTG               CACGAG(-5/-1)
BstEII        139  GGTGACC              G^GT[ACGT]ACC
BstPI         139  GGTGACC              G^GT[ACGT]ACC
BstUI         405  CGCG                 CG^CG
BstXI        1016  CCAATGTCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        533  GCCGGC               [GA]^CCGG[CT]
DraII         939  GGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI          353  CCACGG               C^C[GA][CT]GG
EaeI          383  TGGCCA               [CT]^GGCC[GA]
EarI          559  GAAGAG               CTCTTC(1/4)
EcoO109I      939  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       139  GGTGACC              G^GT[ACGT]ACC
EcoT14I       458  CCAAGG               C^C[AT][AT]GG
FauI          398  GCGGG                CCCGC(4/6)
FspI         1340  TGCGCA               TGC^GCA
Hin1I        1370  GGCGCC               G[GA]^CG[CT]C
KasI         1370  GGCGCC               G^GCGCC
Ksp632I       559  GAAGAG               CTCTTC(1/4)
MaeII        1332  ACGT                 A^CGT
MluNI         383  TGGCCA               TGG^CCA
MscI          383  TGGCCA               TGG^CCA
MseI         1181  TTAA                 T^TAA
MspA1I       1227  CAGCTG               C[AC]G^C[GT]G
MvnI          405  CGCG                 CG^CG
NaeI          533  GCCGGC               GCC^GGC
NarI         1370  GGCGCC               GG^CGCC
NgoMIV        533  GCCGGC               G^CCGGC
PpuMI         939  GGGACCT              [GA]G^G[AT]CC[CT]
PvuII        1227  CAGCTG               CAG^CTG
RsaI          347  GTAC                 GT^AC
SapI          559  GAAGAGC              GCTCTTC(1/4)
SbfI          449  CCTGCAGG             CCTGCA^GG
SfoI         1370  GGCGCC               GGC^GCC
SmaI         1449  CCCGGG               CCC^GGG
SmlI         1180  CTTAAG               C^T[CT][GA]AG
Sse8387I      449  CCTGCAGG             CCTGCA^GG
SspBI         346  TGTACA               T^GTACA
StyI          458  CCAAGG               C^C[AT][AT]GG
Tru9I        1181  TTAA                 T^TAA
Tsp509I       736  AATT                 ^AATT
XcmI          197  CCACTGTGCATATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1449  CCCGGG               C^CCGGG
XmaI         1449  CCCGGG               C^CCGGG
XmnI         1212  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccI           51  GTCTAC               GT^[AC][GT]AC
             1105  GTAGAC              
AlwNI         565  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1475  CAGCCTCTG           
AocI          952  CCTCAGG              CC^T[ACGT]AGG
             1099  CCTCAGG             
AspHI         111  GAGCTC               G[AT]GC[AT]^C
              389  GAGCTC              
AvaII         940  GGACC                G^G[AT]CC
              972  GGTCC               
BseRI         269  CTCCTC               GAGGAG(10/8)
              272  CTCCTC              
BsgI          710  GTGCAG               GTGCAG(16/14)
             1010  GTGCAG              
BsiHKAI       111  GAGCTC               G[AT]GC[AT]^C
              389  GAGCTC              
BspMI         749  ACCTGC               ACCTGC(4/8)
              942  ACCTGC              
BstYI         127  AGATCC               [GA]^GATC[CT]
             1391  AGATCC              
Bsu36I        952  CCTCAGG              CC^T[ACGT]AGG
             1099  CCTCAGG             
Eco81I        952  CCTCAGG              CC^T[ACGT]AGG
             1099  CCTCAGG             
EcoNI         900  CCTAGCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              975  CCTGCCCCAGG         
HaeII         918  AGCGCC               [GA]GCGC^[CT]
             1370  GGCGCC              
MflI          127  AGATCC               [GA]^GATC[CT]
             1391  AGATCC              
MslI          195  CACCACTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              205  CATATGGATG          
NdeI            6  CATATG               CA^TATG
              205  CATATG              
SacI          111  GAGCTC               GAGCT^C
              389  GAGCTC              
SfaNI         211  GATGC                GCATC(5/9)
               62  GCATC               
SphI         1362  GCATGC               GCATG^C
             1469  GCATGC              
TfiI          334  GATTC                G^A[AT]TC
             1430  GAATC               
XhoII         127  AGATCC               [GA]^GATC[CT]
             1391  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AciI        AluI        AlwI        BanII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaJI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeI        MaeIII      MboI        MboII       MnlI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PstI        
Sau3AI      Sau96I      ScrFI       SfcI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     
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