################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrDI BssHII Bst1107I BstAPI BstBI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI HgaI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MroI MunI NcoI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SspI StuI SwaI Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 65 enzymes) # ################################### AccII 405 CGCG CG^CG AcyI 1370 GGCGCC G[GA]^CG[CT]C AfaI 347 GTAC GT^AC AflII 1180 CTTAAG C^TTAAG Asp700I 1212 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 1449 CCCGGG C^[CT]CG[GA]G AviII 1340 TGCGCA TGC^GCA BalI 383 TGGCCA TGG^CCA BanI 1370 GGCGCC G^G[CT][GA]CC BbeI 1370 GGCGCC GGCGC^C BbsI 1155 GAAGAC GAAGAC(2/6) BfrI 1180 CTTAAG C^TTAAG BglI 376 GCCGTAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 1155 GAAGAC GAAGAC(2/6) BsaHI 1370 GGCGCC G[GA]^CG[CT]C BsaI 891 GAGACC GGTCTC(1/5) BsoBI 1449 CCCGGG C^[CT]CG[GA]G Bsp1407I 346 TGTACA T^GTACA BsrBI 396 GAGCGG CCGCTC(-3/-3) BsrFI 533 GCCGGC [GA]^CCGG[CT] BsrGI 346 TGTACA T^GTACA BssSI 289 CTCGTG CACGAG(-5/-1) BstEII 139 GGTGACC G^GT[ACGT]ACC BstPI 139 GGTGACC G^GT[ACGT]ACC BstUI 405 CGCG CG^CG BstXI 1016 CCAATGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 533 GCCGGC [GA]^CCGG[CT] DraII 939 GGGACCT [GA]G^G[ACGT]CC[CT] DsaI 353 CCACGG C^C[GA][CT]GG EaeI 383 TGGCCA [CT]^GGCC[GA] EarI 559 GAAGAG CTCTTC(1/4) EcoO109I 939 GGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 139 GGTGACC G^GT[ACGT]ACC EcoT14I 458 CCAAGG C^C[AT][AT]GG FauI 398 GCGGG CCCGC(4/6) FspI 1340 TGCGCA TGC^GCA Hin1I 1370 GGCGCC G[GA]^CG[CT]C KasI 1370 GGCGCC G^GCGCC Ksp632I 559 GAAGAG CTCTTC(1/4) MaeII 1332 ACGT A^CGT MluNI 383 TGGCCA TGG^CCA MscI 383 TGGCCA TGG^CCA MseI 1181 TTAA T^TAA MspA1I 1227 CAGCTG C[AC]G^C[GT]G MvnI 405 CGCG CG^CG NaeI 533 GCCGGC GCC^GGC NarI 1370 GGCGCC GG^CGCC NgoMIV 533 GCCGGC G^CCGGC PpuMI 939 GGGACCT [GA]G^G[AT]CC[CT] PvuII 1227 CAGCTG CAG^CTG RsaI 347 GTAC GT^AC SapI 559 GAAGAGC GCTCTTC(1/4) SbfI 449 CCTGCAGG CCTGCA^GG SfoI 1370 GGCGCC GGC^GCC SmaI 1449 CCCGGG CCC^GGG SmlI 1180 CTTAAG C^T[CT][GA]AG Sse8387I 449 CCTGCAGG CCTGCA^GG SspBI 346 TGTACA T^GTACA StyI 458 CCAAGG C^C[AT][AT]GG Tru9I 1181 TTAA T^TAA Tsp509I 736 AATT ^AATT XcmI 197 CCACTGTGCATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1449 CCCGGG C^CCGGG XmaI 1449 CCCGGG C^CCGGG XmnI 1212 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 51 GTCTAC GT^[AC][GT]AC 1105 GTAGAC AlwNI 565 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 1475 CAGCCTCTG AocI 952 CCTCAGG CC^T[ACGT]AGG 1099 CCTCAGG AspHI 111 GAGCTC G[AT]GC[AT]^C 389 GAGCTC AvaII 940 GGACC G^G[AT]CC 972 GGTCC BseRI 269 CTCCTC GAGGAG(10/8) 272 CTCCTC BsgI 710 GTGCAG GTGCAG(16/14) 1010 GTGCAG BsiHKAI 111 GAGCTC G[AT]GC[AT]^C 389 GAGCTC BspMI 749 ACCTGC ACCTGC(4/8) 942 ACCTGC BstYI 127 AGATCC [GA]^GATC[CT] 1391 AGATCC Bsu36I 952 CCTCAGG CC^T[ACGT]AGG 1099 CCTCAGG Eco81I 952 CCTCAGG CC^T[ACGT]AGG 1099 CCTCAGG EcoNI 900 CCTAGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 975 CCTGCCCCAGG HaeII 918 AGCGCC [GA]GCGC^[CT] 1370 GGCGCC MflI 127 AGATCC [GA]^GATC[CT] 1391 AGATCC MslI 195 CACCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 205 CATATGGATG NdeI 6 CATATG CA^TATG 205 CATATG SacI 111 GAGCTC GAGCT^C 389 GAGCTC SfaNI 211 GATGC GCATC(5/9) 62 GCATC SphI 1362 GCATGC GCATG^C 1469 GCATGC TfiI 334 GATTC G^A[AT]TC 1430 GAATC XhoII 127 AGATCC [GA]^GATC[CT] 1391 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AluI AlwI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI Sau3AI Sau96I ScrFI SfcI TaqI TseI Tsp45I TspRI TthHB8I