Restriction map for
pF1KB7283
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# NO CUT ENZYMES ( 94 enzymes) #
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AccI AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI Asp700I AspEI AviII AvrII
BamHI BbeI BbrPI BbsI BcgI
BfrI BlnI BpuAI BsaAI BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspEI BspLU11I BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV FseI
FspI HaeII KasI KspI MamI
MfeI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 1531 GACGTC GACGT^C
AcyI 1531 GACGTC G[GA]^CG[CT]C
AflIII 2001 ACGCGT A^C[GA][CT]GT
ApaI 2508 GGGCCC GGGCC^C
AseI 1927 ATTAAT AT^TAAT
AsnI 1927 ATTAAT AT^TAAT
AspI 1710 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 644 GTATCC GTATCC(6/5)
BclI 2231 TGATCA T^GATCA
BfaI 769 CTAG C^TAG
BglI 1916 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2201 AGATCT A^GATCT
BlpI 106 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 106 GCTCAGC GC^T[ACGT]AGC
BsaHI 1531 GACGTC G[GA]^CG[CT]C
BsiEI 1024 CGATCG CG[GA][CT]^CG
BspDI 1026 ATCGAT AT^CGAT
BsrDI 450 CATTGC GCAATG(2/0)
BssHII 126 GCGCGC G^CGCGC
BstXI 1742 CCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 106 GCTCAGC GC^T[ACGT]AGC
ClaI 1026 ATCGAT AT^CGAT
EcoT22I 1151 ATGCAT ATGCA^T
FbaI 2231 TGATCA T^GATCA
HgaI 1472 GACGC GACGC(5/10)
Hin1I 1531 GACGTC G[GA]^CG[CT]C
HincII 736 GTTAAC GT[CT]^[GA]AC
HindII 736 GTTAAC GT[CT]^[GA]AC
HindIII 1543 AAGCTT A^AGCTT
HpaI 736 GTTAAC GTT^AAC
MaeI 769 CTAG C^TAG
MluI 2001 ACGCGT A^CGCGT
NdeI 1829 CATATG CA^TATG
NsiI 1151 ATGCAT ATGCA^T
PflFI 1710 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1927 ATTAAT AT^TAAT
PstI 1649 CTGCAG CTGCA^G
PvuI 1024 CGATCG CGAT^CG
ScaI 896 AGTACT AGT^ACT
SexAI 8 ACCAGGT A^CC[AT]GGT
SphI 797 GCATGC GCATG^C
SspI 759 AATATT AAT^ATT
Tth111I 1710 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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Acc65I 953 GGTACC G^GTACC
1286 GGTACC
Asp718I 953 GGTACC G^GTACC
1286 GGTACC
BsgI 902 CTGCAC GTGCAG(16/14)
1642 CTGCAC
BspHI 1700 TCATGA T^CATGA
1784 TCATGA
BspMI 232 ACCTGC ACCTGC(4/8)
426 GCAGGT
BsrBI 211 GAGCGG CCGCTC(-3/-3)
277 GAGCGG
BsrFI 956 ACCGGC [GA]^CCGG[CT]
2076 GCCGGT
Cfr10I 956 ACCGGC [GA]^CCGG[CT]
2076 GCCGGT
EcoRI 1125 GAATTC G^AATTC
1696 GAATTC
KpnI 953 GGTACC GGTAC^C
1286 GGTACC
MslI 1926 CATTAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2088 CACCATCATG
NspI 797 GCATGC [GA]CATG^[CT]
1721 ACATGC
RcaI 1700 TCATGA T^CATGA
1784 TCATGA
SacI 104 GAGCTC GAGCT^C
499 GAGCTC
SapI 193 GAAGAGC GCTCTTC(1/4)
2058 GCTCTTC
SfcI 1002 CTACAG C^T[GA][CT]AG
1649 CTGCAG
SmaI 147 CCCGGG CCC^GGG
1661 CCCGGG
SmlI 57 CTCAAG C^T[CT][GA]AG
1540 CTCAAG
StuI 1914 AGGCCT AGG^CCT
2226 AGGCCT
XcmI 1418 CCATCGCTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1440 CCACCCACGCACTGG
XmaCI 147 CCCGGG C^CCGGG
1661 CCCGGG
XmaI 147 CCCGGG C^CCGGG
1661 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII