Restriction map for
pF1KB7294
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII Acc65I AclI AcsI AflII
AgeI Aor51HI ApoI AscI AseI
AsnI Asp718I AspI AviII AvrII
BalI BamHI BbeI BbvCI BcgI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsiWI BsmI Bsp1407I BspDI
BspHI BsrBI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI Eco47III EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MscI MslI
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 940 GTCTAC GT^[AC][GT]AC
AccIII 2296 TCCGGA T^CCGGA
AcyI 1444 GACGCC G[GA]^CG[CT]C
AflIII 1973 ACATGT A^C[GA][CT]GT
AhdI 69 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 700 GTGCAC G^TGCAC
ApaI 231 GGGCCC GGGCC^C
ApaLI 700 GTGCAC G^TGCAC
Asp700I 1344 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 69 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 2194 GGCACC G^G[CT][GA]CC
BbrPI 1909 CACGTG CAC^GTG
BbsI 472 GTCTTC GAAGAC(2/6)
BciVI 2129 GTATCC GTATCC(6/5)
BclI 734 TGATCA T^GATCA
BglI 631 GCCCAGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 472 GTCTTC GAAGAC(2/6)
BsaHI 1444 GACGCC G[GA]^CG[CT]C
BseAI 2296 TCCGGA T^CCGGA
BsiEI 652 CGGCCG CG[GA][CT]^CG
BsmBI 2167 GAGACG CGTCTC(1/5)
BspEI 2296 TCCGGA T^CCGGA
BspLU11I 1973 ACATGT A^CATGT
BssSI 1011 CACGAG CACGAG(-5/-1)
DraI 2272 TTTAAA TTT^AAA
DraIII 1493 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 652 CGGCCG C^GGCCG
Eam1105I 69 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 652 CGGCCG C^GGCCG
Eco52I 652 CGGCCG C^GGCCG
EcoNI 1828 CCTCGGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 734 TGATCA T^GATCA
Hin1I 1444 GACGCC G[GA]^CG[CT]C
HincII 1810 GTCAAC GT[CT]^[GA]AC
HindII 1810 GTCAAC GT[CT]^[GA]AC
MroI 2296 TCCGGA T^CCGGA
MseI 2273 TTAA T^TAA
NcoI 866 CCATGG C^CATGG
NdeI 1041 CATATG CA^TATG
NspI 1973 ACATGT [GA]CATG^[CT]
PmaCI 1909 CACGTG CAC^GTG
PmlI 1909 CACGTG CAC^GTG
SexAI 1841 ACCTGGT A^CC[AT]GGT
SmaI 1646 CCCGGG CCC^GGG
SmlI 1315 CTCAAG C^T[CT][GA]AG
Tru9I 2273 TTAA T^TAA
Tsp509I 729 AATT ^AATT
XmaCI 1646 CCCGGG C^CCGGG
XmaI 1646 CCCGGG C^CCGGG
XmnI 1344 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 133 CCTGAGG CC^T[ACGT]AGG
538 CCTCAGG
AvaI 990 CTCGGG C^[CT]CG[GA]G
1646 CCCGGG
BfaI 1559 CTAG C^TAG
1834 CTAG
BsoBI 990 CTCGGG C^[CT]CG[GA]G
1646 CCCGGG
BsrDI 2217 CATTGC GCAATG(2/0)
2078 GCAATG
BsrFI 650 ACCGGC [GA]^CCGG[CT]
1652 ACCGGC
Bsu36I 133 CCTGAGG CC^T[ACGT]AGG
538 CCTCAGG
Cfr10I 650 ACCGGC [GA]^CCGG[CT]
1652 ACCGGC
DrdI 202 GACACAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
947 GACGGGAGGGTC
EarI 985 CTCTTC CTCTTC(1/4)
1300 CTCTTC
Eco81I 133 CCTGAGG CC^T[ACGT]AGG
538 CCTCAGG
HgaI 1116 GACGC GACGC(5/10)
1444 GACGC
HinfI 1437 GACTC G^A[ACGT]TC
1555 GACTC
Ksp632I 985 CTCTTC CTCTTC(1/4)
1300 CTCTTC
MaeI 1559 CTAG C^TAG
1834 CTAG
PflMI 510 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1113 CCAGACGCTGG
PleI 1437 GACTC GAGTC(4/5)
1555 GACTC
TaqI 755 TCGA T^CGA
1256 TCGA
TthHB8I 755 TCGA T^CGA
1256 TCGA
Van91I 510 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1113 CCAGACGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BanII BbvI
BcnI BmyI BpmI BsaAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TseI Tsp45I TspRI
XcmI XhoII