Restriction map for
pF1KB7298
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# NO CUT ENZYMES ( 75 enzymes) #
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AatII Acc65I AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BclI BfrI BlnI BsiWI
BsmI Bsp1407I BspDI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I ClaI CpoI DraI DrdI
Eam1105I Eco47III EcoRI EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
MfeI MluI MseI MunI NdeI
NheI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SalI ScaI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI Tru9I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 127 GTCTAC GT^[AC][GT]AC
AccIII 2460 TCCGGA T^CCGGA
AcsI 490 AAATTT [GA]^AATT[CT]
AocI 1995 CCTGAGG CC^T[ACGT]AGG
ApaI 1697 GGGCCC GGGCC^C
ApoI 490 AAATTT [GA]^AATT[CT]
Asp700I 1356 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 1554 CACGTG CAC^GTG
BciVI 2847 GGATAC GTATCC(6/5)
BfaI 826 CTAG C^TAG
BsaAI 1554 CACGTG [CT]AC^GT[GA]
BsaBI 339 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2460 TCCGGA [AT]^CCGG[AT]
BseAI 2460 TCCGGA T^CCGGA
BspEI 2460 TCCGGA T^CCGGA
BspHI 8 TCATGA T^CATGA
BsrDI 1626 CATTGC GCAATG(2/0)
BstEII 723 GGTCACC G^GT[ACGT]ACC
BstPI 723 GGTCACC G^GT[ACGT]ACC
Bsu36I 1995 CCTGAGG CC^T[ACGT]AGG
DraIII 1399 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1516 CGGCCG C^GGCCG
EarI 817 CTCTTC CTCTTC(1/4)
EclXI 1516 CGGCCG C^GGCCG
Eco52I 1516 CGGCCG C^GGCCG
Eco81I 1995 CCTGAGG CC^T[ACGT]AGG
EcoO65I 723 GGTCACC G^GT[ACGT]ACC
HincII 1564 GTCAAC GT[CT]^[GA]AC
HindII 1564 GTCAAC GT[CT]^[GA]AC
Ksp632I 817 CTCTTC CTCTTC(1/4)
MaeI 826 CTAG C^TAG
MamI 339 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2460 TCCGGA T^CCGGA
NaeI 2642 GCCGGC GCC^GGC
NcoI 2891 CCATGG C^CATGG
NgoMIV 2642 GCCGGC G^CCGGC
NotI 1515 GCGGCCGC GC^GGCCGC
NruI 778 TCGCGA TCG^CGA
PleI 2497 GACTC GAGTC(4/5)
PmaCI 1554 CACGTG CAC^GTG
PmlI 1554 CACGTG CAC^GTG
PshAI 2464 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 8 TCATGA T^CATGA
SacI 151 GAGCTC GAGCT^C
SapI 816 GCTCTTC GCTCTTC(1/4)
SbfI 1767 CCTGCAGG CCTGCA^GG
SexAI 919 ACCTGGT A^CC[AT]GGT
SmaI 2042 CCCGGG CCC^GGG
SphI 1532 GCATGC GCATG^C
Sse8387I 1767 CCTGCAGG CCTGCA^GG
XcmI 2594 CCAGCCCAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2042 CCCGGG C^CCGGG
XmaI 2042 CCCGGG C^CCGGG
XmnI 1356 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
Alw44I 997 GTGCAC G^TGCAC
1501 GTGCAC
AlwI 1650 GATCC GGATC(4/5)
2105 GATCC
ApaLI 997 GTGCAC G^TGCAC
1501 GTGCAC
BcgI 933 CGACCTTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1635 GCACCTGAATCG
BglII 1358 AGATCT A^GATCT
2657 AGATCT
BsiEI 165 CGGTCG CG[GA][CT]^CG
1516 CGGCCG
BsmBI 669 CGTCTC CGTCTC(1/5)
2665 CGTCTC
KspI 438 CCGCGG CCGC^GG
1513 CCGCGG
NspI 1439 GCATGT [GA]CATG^[CT]
1532 GCATGC
SacII 438 CCGCGG CCGC^GG
1513 CCGCGG
StuI 2542 AGGCCT AGG^CCT
2987 AGGCCT
TfiI 1641 GAATC G^A[AT]TC
2531 GATTC
Tsp509I 491 AATT ^AATT
2603 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoNI EcoO109I EcoRII EcoRV
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PflMI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfiI SfoI SmlI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XhoII