################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AccI AccIII AclI AflII AscI AseI AsnI Asp700I AvrII BamHI BcgI BciVI BclI BfaI BfrI BglII BlnI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DraI DraIII DrdI EcoRV FbaI FseI HindIII HpaI MaeI MfeI MroI MunI NdeI NheI NotI NruI NspV PacI PmeI PshBI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SexAI SfuI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AcsI 982 GAATTC [GA]^AATT[CT] AgeI 2508 ACCGGT A^CCGGT AhdI 2752 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 737 AGCGCT AGC^GCT ApoI 982 GAATTC [GA]^AATT[CT] AspEI 2752 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3532 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 677 TGCGCA TGC^GCA BbvCI 1439 CCTCAGC CCTCAGC(-5/-2) BsaBI 1177 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2508 ACCGGT [AT]^CCGG[AT] BspHI 2234 TCATGA T^CATGA BspLU11I 2897 ACATGT A^CATGT BsrDI 2241 CATTGC GCAATG(2/0) BstYI 3446 AGATCC [GA]^GATC[CT] Eam1105I 2752 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 737 AGCGCT AGC^GCT EcoRI 982 GAATTC G^AATTC EcoT22I 1484 ATGCAT ATGCA^T FspI 677 TGCGCA TGC^GCA HincII 1300 GTCAAC GT[CT]^[GA]AC HindII 1300 GTCAAC GT[CT]^[GA]AC MamI 1177 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 3446 AGATCC [GA]^GATC[CT] MluI 414 ACGCGT A^CGCGT MseI 97 TTAA T^TAA NsiI 1484 ATGCAT ATGCA^T PflFI 3532 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 2508 ACCGGT A^CCGGT PshAI 208 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 2234 TCATGA T^CATGA SacI 2815 GAGCTC GAGCT^C SgrAI 2507 CACCGGTG C[GA]^CCGG[CT]G Tru9I 97 TTAA T^TAA Tsp509I 983 AATT ^AATT Tth111I 3532 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 3446 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AatII 58 GACGTC GACGT^C 3696 GACGTC Acc65I 46 GGTACC G^GTACC 3461 GGTACC AlwI 3447 GATCC GGATC(4/5) 3657 GATCC AocI 376 CCTGAGG CC^T[ACGT]AGG 1258 CCTGAGG Asp718I 46 GGTACC G^GTACC 3461 GGTACC BsmBI 2392 GAGACG CGTCTC(1/5) 3352 GAGACG BstEII 2130 GGTGACC G^GT[ACGT]ACC 3702 GGTGACC BstPI 2130 GGTGACC G^GT[ACGT]ACC 3702 GGTGACC Bsu36I 376 CCTGAGG CC^T[ACGT]AGG 1258 CCTGAGG EarI 1078 CTCTTC CTCTTC(1/4) 391 GAAGAG Eco81I 376 CCTGAGG CC^T[ACGT]AGG 1258 CCTGAGG EcoO65I 2130 GGTGACC G^GT[ACGT]ACC 3702 GGTGACC KpnI 46 GGTACC GGTAC^C 3461 GGTACC Ksp632I 1078 CTCTTC CTCTTC(1/4) 391 GAAGAG KspI 3079 CCGCGG CCGC^GG 3639 CCGCGG PaeR7I 542 CTCGAG C^TCGAG 1936 CTCGAG SacII 3079 CCGCGG CCGC^GG 3639 CCGCGG SphI 179 GCATGC GCATG^C 1482 GCATGC StuI 1262 AGGCCT AGG^CCT 2849 AGGCCT TfiI 2409 GATTC G^A[AT]TC 3681 GAATC XhoI 542 CTCGAG C^TCGAG 1936 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI Alw44I AlwNI ApaI ApaLI AspHI AvaI AvaII BalI BanI BanII BbeI BbrPI BbsI BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SfoI SmaI SmlI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI XmaCI XmaI