Restriction map for
pF1KB7359
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BseRI BsiEI BsiWI BsoBI
Bsp1407I BspDI BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MunI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
TfiI Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccIII 914 TCCGGA T^CCGGA
AflIII 308 ACATGT A^C[GA][CT]GT
Alw44I 60 GTGCAC G^TGCAC
AlwI 493 GGATC GGATC(4/5)
ApaLI 60 GTGCAC G^TGCAC
Asp700I 208 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 60 GTGCAC G[AT]GC[AT]^C
AspI 328 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 885 GGACC G^G[AT]CC
BalI 975 TGGCCA TGG^CCA
BcgI 386 CGAAAAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 60 GTGCAC G[AGT]GC[ACT]^C
BsaJI 1010 CCCAGG C^C[ACGT][ACGT]GG
BsaWI 914 TCCGGA [AT]^CCGG[AT]
BseAI 914 TCCGGA T^CCGGA
BsgI 941 GTGCAG GTGCAG(16/14)
BsiHKAI 60 GTGCAC G[AT]GC[AT]^C
BsiYI 114 CCATTTTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 114 CCATTTTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 14 CGTCTC CGTCTC(1/5)
BsmFI 674 GGGAC GGGAC(10/14)
Bsp1286I 60 GTGCAC G[AGT]GC[ACT]^C
BspEI 914 TCCGGA T^CCGGA
BspHI 441 TCATGA T^CATGA
BspLU11I 308 ACATGT A^CATGT
BsrBI 236 CCGCTC CCGCTC(-3/-3)
BstAPI 149 GCAACACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 493 GGATCT [GA]^GATC[CT]
DraI 588 TTTAAA TTT^AAA
EaeI 975 TGGCCA [CT]^GGCC[GA]
EarI 559 GAAGAG CTCTTC(1/4)
EcoRV 37 GATATC GAT^ATC
HgaI 703 GACGC GACGC(5/10)
HinfI 322 GACTC G^A[ACGT]TC
Ksp632I 559 GAAGAG CTCTTC(1/4)
MaeII 47 ACGT A^CGT
MflI 493 GGATCT [GA]^GATC[CT]
MluNI 975 TGGCCA TGG^CCA
MroI 914 TCCGGA T^CCGGA
MscI 975 TGGCCA TGG^CCA
MslI 955 CATTTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 482 CATATG CA^TATG
NlaIV 104 GGAACC GG[ACGT]^[ACGT]CC
PflFI 328 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 322 GACTC GAGTC(4/5)
PvuII 550 CAGCTG CAG^CTG
RcaI 441 TCATGA T^CATGA
SapI 559 GAAGAGC GCTCTTC(1/4)
SspI 345 AATATT AAT^ATT
TaqI 35 TCGA T^CGA
Tth111I 328 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 35 TCGA T^CGA
XcmI 340 CCAGAAATATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 493 GGATCT [GA]^GATC[CT]
XmnI 208 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 199 CCGC CCGC(-3/-1)
236 CCGC
AfaI 459 GTAC GT^AC
833 GTAC
BbsI 622 GTCTTC GAAGAC(2/6)
631 GTCTTC
BcnI 221 CCGGG CC^[GC]GG
470 CCCGG
BpuAI 622 GTCTTC GAAGAC(2/6)
631 GTCTTC
BsmI 165 GAATGC GAATGC(1/-1)
661 GAATGC
BstNI 713 CCTGG CC^[AT]GG
1011 CCAGG
Cfr13I 770 GGGCC G^G[ACGT]CC
885 GGACC
DpnII 479 GATC ^GATC
494 GATC
EcoRI 412 GAATTC G^AATTC
999 GAATTC
EcoRII 713 CCTGG ^CC[AT]GG
1011 CCAGG
MaeIII 127 GTCAC ^GT[ACGT]AC
1006 GTCAC
MboI 479 GATC ^GATC
494 GATC
MseI 89 TTAA T^TAA
589 TTAA
MspA1I 199 CCGCTG C[AC]G^C[GT]G
550 CAGCTG
MvaI 713 CCTGG CC^[AT]GG
1011 CCAGG
MwoI 149 GCAACACATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
763 GCTGTGAGGGC
NciI 221 CCGGG CC^[GC]GG
470 CCCGG
NdeII 479 GATC ^GATC
494 GATC
NspI 154 ACATGC [GA]CATG^[CT]
308 ACATGT
RsaI 459 GTAC GT^AC
833 GTAC
Sau3AI 479 GATC ^GATC
494 GATC
Sau96I 770 GGGCC G^G[ACGT]CC
885 GGACC
Tru9I 89 TTAA T^TAA
589 TTAA
Tsp45I 127 GTCAC ^GT[GC]AC
1006 GTCAC
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AcsI AluI ApoI BbvI BsmAI
BsrI BssKI BstF5I DdeI Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MboII MnlI MspI NlaIII
PstI ScrFI SfaNI SfcI TseI
Tsp509I TspRI