################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DraII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MunI NarI NcoI NdeI NdeII NheI NotI NruI NspI NspV PacI PaeR7I PflMI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tsp45I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccII 776 CGCG CG^CG AspI 51 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 160 GGGCTC G[GA]GC[CT]^C BbrPI 714 CACGTG CAC^GTG BbvI 287 GCTGC GCAGC(8/12) BcnI 236 CCCGG CC^[GC]GG BmyI 160 GGGCTC G[AGT]GC[ACT]^C BsaAI 714 CACGTG [CT]AC^GT[GA] BseRI 323 GAGGAG GAGGAG(10/8) BsmAI 57 GTCTC GTCTC(1/5) BsmFI 541 GTCCC GGGAC(10/14) Bsp1286I 160 GGGCTC G[AGT]GC[ACT]^C BspHI 404 TCATGA T^CATGA BspMI 418 ACCTGC ACCTGC(4/8) BsrDI 427 GCAATG GCAATG(2/0) BsrFI 380 GCCGGC [GA]^CCGG[CT] BsrI 54 CCAGT ACTGG(1/-1) BstUI 776 CGCG CG^CG CfoI 777 GCGC GCG^C Cfr10I 380 GCCGGC [GA]^CCGG[CT] DraIII 823 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 817 CTCTTC CTCTTC(1/4) EcoRI 31 GAATTC G^AATTC EcoT22I 209 ATGCAT ATGCA^T Fnu4HI 287 GCTGC GC^[ACGT]GC HhaI 777 GCGC GCG^C HinP1I 777 GCGC G^CGC HindIII 884 AAGCTT A^AGCTT HinfI 846 GAATC G^A[ACGT]TC ItaI 287 GCTGC GC^[ACGT]GC Ksp632I 817 CTCTTC CTCTTC(1/4) MaeII 715 ACGT A^CGT MvnI 776 CGCG CG^CG NaeI 380 GCCGGC GCC^GGC NciI 236 CCCGG CC^[GC]GG NgoMIV 380 GCCGGC G^CCGGC NlaIV 231 GGCTCC GG[ACGT]^[ACGT]CC NsiI 209 ATGCAT ATGCA^T PflFI 51 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 714 CACGTG CAC^GTG PmlI 714 CACGTG CAC^GTG RcaI 404 TCATGA T^CATGA SfcI 279 CTACAG C^T[GA][CT]AG SmlI 59 CTCAAG C^T[CT][GA]AG SspI 96 AATATT AAT^ATT TfiI 846 GAATC G^A[AT]TC TseI 287 GCTGC G^C[AT]GC Tth111I 51 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 467 CCATTATCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) 492 CCGC AcsI 31 GAATTC [GA]^AATT[CT] 123 AAATTT ApoI 31 GAATTC [GA]^AATT[CT] 123 AAATTT AvaII 480 GGTCC G^G[AT]CC 754 GGACC BbsI 598 GTCTTC GAAGAC(2/6) 681 GTCTTC BfaI 88 CTAG C^TAG 413 CTAG BpuAI 598 GTCTTC GAAGAC(2/6) 681 GTCTTC BsgI 388 CTGCAC GTGCAG(16/14) 564 CTGCAC DdeI 296 CTCAG C^T[ACGT]AG 689 CTGAG EcoT14I 42 CCTTGG C^C[AT][AT]GG 318 CCTTGG HapII 237 CCGG C^CGG 381 CCGG HpaII 237 CCGG C^CGG 381 CCGG HphI 140 TCACC GGTGA(8/7) 722 TCACC MaeI 88 CTAG C^TAG 413 CTAG MseI 94 TTAA T^TAA 905 TTAA MslI 573 CACATTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 620 CACTCCTGTG MspA1I 517 CAGCTG C[AC]G^C[GT]G 797 CAGCTG MspI 237 CCGG C^CGG 381 CCGG MwoI 646 GCCTCCTATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 790 GCTTTCCCAGC PvuII 517 CAGCTG CAG^CTG 797 CAGCTG StyI 42 CCTTGG C^C[AT][AT]GG 318 CCTTGG Tru9I 94 TTAA T^TAA 905 TTAA Tsp509I 32 AATT ^AATT 124 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AluI AlwNI BsaJI BsiYI BslI BssKI BstF5I BstNI Cac8I Cfr13I EcoRII FokI HaeIII MboII MnlI MvaI NlaIII Sau96I ScrFI SfaNI TspRI