################################## # NO CUT ENZYMES ( 177 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AciI 357 CCGC CCGC(-3/-1) AlwNI 314 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 598 ATTAAT AT^TAAT AsnI 598 ATTAAT AT^TAAT AspHI 408 GAGCAC G[AT]GC[AT]^C BanI 478 GGCACC G^G[CT][GA]CC BbsI 655 GTCTTC GAAGAC(2/6) BclI 134 TGATCA T^GATCA BpuAI 655 GTCTTC GAAGAC(2/6) BsgI 382 CTGCAC GTGCAG(16/14) BsiHKAI 408 GAGCAC G[AT]GC[AT]^C BsmAI 786 GTCTC GTCTC(1/5) BsmI 72 GCATTC GAATGC(1/-1) BspHI 131 TCATGA T^CATGA BsrDI 759 CATTGC GCAATG(2/0) BssKI 291 CCAGG ^CC[ACGT]GG BstNI 291 CCAGG CC^[AT]GG BstXI 878 CCATAAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 561 CTCAG C^T[ACGT]AG EarI 82 CTCTTC CTCTTC(1/4) EcoRII 291 CCAGG ^CC[AT]GG EcoT14I 276 CCTTGG C^C[AT][AT]GG FbaI 134 TGATCA T^GATCA Ksp632I 82 CTCTTC CTCTTC(1/4) MaeII 653 ACGT A^CGT MspA1I 473 CAGCTG C[AC]G^C[GT]G MvaI 291 CCAGG CC^[AT]GG PleI 784 GAGTC GAGTC(4/5) PshBI 598 ATTAAT AT^TAAT PstI 320 CTGCAG CTGCA^G PvuII 473 CAGCTG CAG^CTG RcaI 131 TCATGA T^CATGA ScrFI 291 CCAGG CC^[ACGT]GG StyI 276 CCTTGG C^C[AT][AT]GG TaqI 76 TCGA T^CGA TthHB8I 76 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcsI 25 GAATTT [GA]^AATT[CT] 567 AAATTT AlwI 675 GATCC GGATC(4/5) 201 GGATC ApoI 25 GAATTT [GA]^AATT[CT] 567 AAATTT BbvI 327 GCTGC GCAGC(8/12) 831 GCTGC BmyI 104 GTGCCC G[AGT]GC[ACT]^C 408 GAGCAC BsaJI 276 CCTTGG C^C[ACGT][ACGT]GG 290 CCCAGG BseRI 331 CTCCTC GAGGAG(10/8) 334 CTCCTC Bsp1286I 104 GTGCCC G[AGT]GC[ACT]^C 408 GAGCAC BstYI 201 GGATCT [GA]^GATC[CT] 674 AGATCC Cac8I 475 GCTGGC GC[ACGT]^[ACGT]GC 774 GCCAGC Fnu4HI 327 GCTGC GC^[ACGT]GC 831 GCTGC HaeIII 691 GGCC GG^CC 876 GGCC ItaI 327 GCTGC GC^[ACGT]GC 831 GCTGC MflI 201 GGATCT [GA]^GATC[CT] 674 AGATCC SfcI 320 CTGCAG C^T[GA][CT]AG 662 CTACAG TseI 327 GCTGC G^C[AT]GC 831 GCTGC TspRI 429 CACTG CA[GC]TG[ACGT][ACGT]^ 750 CACTG XhoII 201 GGATCT [GA]^GATC[CT] 674 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AluI AvaII BsiYI BslI BsrI BstF5I Cfr13I DpnII FokI HinfI HphI MaeIII MboI MboII MnlI MseI MslI NdeII NlaIII NlaIV Sau3AI Sau96I SfaNI TfiI Tru9I Tsp45I Tsp509I