Restriction map for
pF1KB7362
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BglI BlnI
BlpI Bpu1102I BsaBI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
FbaI FseI FspI HincII HindII
HindIII HpaI HphI KpnI MaeIII
MamI MfeI MluI MluNI MroI
MscI MunI NdeI NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tsp45I Tsp509I Tth111I TthHB8I Van91I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcyI 97 GGCGCC G[GA]^CG[CT]C
AflII 543 CTTAAG C^TTAAG
BbeI 97 GGCGCC GGCGC^C
BfrI 543 CTTAAG C^TTAAG
BglII 237 AGATCT A^GATCT
BpmI 663 CTGGAG CTGGAG(16/14)
BsaAI 109 TACGTG [CT]AC^GT[GA]
BsaHI 97 GGCGCC G[GA]^CG[CT]C
BsmAI 147 GTCTC GTCTC(1/5)
BsrBI 358 GAGCGG CCGCTC(-3/-3)
BsrFI 69 GCCGGC [GA]^CCGG[CT]
BsrI 662 ACTGG ACTGG(1/-1)
BstXI 572 CCAAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 237 AGATCT [GA]^GATC[CT]
Cfr10I 69 GCCGGC [GA]^CCGG[CT]
DpnII 238 GATC ^GATC
DraII 103 GGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 376 GAAGAG CTCTTC(1/4)
EcoNI 117 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 103 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 741 ATGCAT ATGCA^T
HgaI 539 GACGC GACGC(5/10)
Hin1I 97 GGCGCC G[GA]^CG[CT]C
HinfI 145 GAGTC G^A[ACGT]TC
KasI 97 GGCGCC G^GCGCC
Ksp632I 376 GAAGAG CTCTTC(1/4)
KspI 51 CCGCGG CCGC^GG
MaeII 110 ACGT A^CGT
MboI 238 GATC ^GATC
MflI 237 AGATCT [GA]^GATC[CT]
NaeI 69 GCCGGC GCC^GGC
NarI 97 GGCGCC GG^CGCC
NdeII 238 GATC ^GATC
NgoMIV 69 GCCGGC G^CCGGC
NsiI 741 ATGCAT ATGCA^T
PleI 145 GAGTC GAGTC(4/5)
PstI 313 CTGCAG CTGCA^G
SacII 51 CCGCGG CCGC^GG
Sau3AI 238 GATC ^GATC
SfaNI 476 GATGC GCATC(5/9)
SfcI 313 CTGCAG C^T[GA][CT]AG
SfoI 97 GGCGCC GGC^GCC
SmlI 543 CTTAAG C^T[CT][GA]AG
XbaI 591 TCTAGA T^CTAGA
XhoII 237 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AluI 265 AGCT AG^CT
411 AGCT
ApaI 103 GGGCCC GGGCC^C
134 GGGCCC
AvaI 58 CCCGAG C^[CT]CG[GA]G
91 CCCGAG
AvaII 114 GGACC G^G[AT]CC
339 GGTCC
BanI 97 GGCGCC G^G[CT][GA]CC
499 GGCACC
BbsI 634 GAAGAC GAAGAC(2/6)
726 GAAGAC
BfaI 43 CTAG C^TAG
592 CTAG
BpuAI 634 GAAGAC GAAGAC(2/6)
726 GAAGAC
BsoBI 58 CCCGAG C^[CT]CG[GA]G
91 CCCGAG
BssHII 6 GCGCGC G^CGCGC
8 GCGCGC
BstNI 117 CCTGG CC^[AT]GG
278 CCAGG
EcoRII 117 CCTGG ^CC[AT]GG
278 CCAGG
EcoT14I 299 CCATGG C^C[AT][AT]GG
441 CCATGG
MaeI 43 CTAG C^TAG
592 CTAG
MslI 734 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
744 CATAGATGTG
MspA1I 51 CCGCGG C[AC]G^C[GT]G
490 CCGCTG
MvaI 117 CCTGG CC^[AT]GG
278 CCAGG
NcoI 299 CCATGG C^CATGG
441 CCATGG
StyI 299 CCATGG C^C[AT][AT]GG
441 CCATGG
TspRI 311 CACTG CA[GC]TG[ACGT][ACGT]^
661 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AccII AciI AspHI BanII BbvI
BcnI BmyI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BssKI BstF5I BstUI
Cac8I CfoI Cfr13I DdeI DsaI
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII ItaI
MboII MnlI MseI MspI MvnI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI Tru9I TseI