Restriction map for
pF1KB7363
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BciVI BclI BfaI BfrI BglI
BlnI BpuAI BsaAI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstF5I BstZ17I ClaI
CpoI DraI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoRV EcoT22I FbaI FokI FseI
FspI HincII HindII HindIII HpaI
KasI Ksp632I KspI MaeI MluI
MluNI MroI MscI MseI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SwaI TfiI Tru9I
Tth111I Van91I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 1079 GGTACC G^GTACC
AfaI 1080 GTAC GT^AC
AlwI 176 GATCC GGATC(4/5)
AlwNI 836 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 274 CCTAAGG CC^T[ACGT]AGG
ApaI 1047 GGGCCC GGGCC^C
Asp718I 1079 GGTACC G^GTACC
BcgI 1022 GCACCGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 329 AGATCT A^GATCT
BpmI 887 CTCCAG CTGGAG(16/14)
BsaBI 63 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 596 GGTCTC GGTCTC(1/5)
BspHI 513 TCATGA T^CATGA
BsrBI 745 GAGCGG CCGCTC(-3/-3)
BssSI 247 CACGAG CACGAG(-5/-1)
BstEII 652 GGTTACC G^GT[ACGT]ACC
BstPI 652 GGTTACC G^GT[ACGT]ACC
BstXI 1035 CCACAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 274 CCTAAGG CC^T[ACGT]AGG
DrdI 976 GACTCGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 274 CCTAAGG CC^T[ACGT]AGG
EcoO65I 652 GGTTACC G^GT[ACGT]ACC
EcoRI 733 GAATTC G^AATTC
EcoT14I 944 CCATGG C^C[AT][AT]GG
KpnI 1079 GGTACC GGTAC^C
MamI 63 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 266 CAATTG C^AATTG
MunI 266 CAATTG C^AATTG
NcoI 944 CCATGG C^CATGG
NspI 644 GCATGC [GA]CATG^[CT]
PaeR7I 599 CTCGAG C^TCGAG
PpuMI 158 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 513 TCATGA T^CATGA
RsaI 1080 GTAC GT^AC
SmaI 587 CCCGGG CCC^GGG
SmlI 599 CTCGAG C^T[CT][GA]AG
SphI 644 GCATGC GCATG^C
SspI 380 AATATT AAT^ATT
StuI 699 AGGCCT AGG^CCT
StyI 944 CCATGG C^C[AT][AT]GG
TspRI 801 CACTG CA[GC]TG[ACGT][ACGT]^
XhoI 599 CTCGAG C^TCGAG
XmaCI 587 CCCGGG C^CCGGG
XmaI 587 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 503 AAATTC [GA]^AATT[CT]
733 GAATTC
AcyI 409 GACGCC G[GA]^CG[CT]C
622 GACGCC
ApoI 503 AAATTC [GA]^AATT[CT]
733 GAATTC
BanI 443 GGCACC G^G[CT][GA]CC
1079 GGTACC
BlpI 550 GCTCAGC GC^T[ACGT]AGC
581 GCTCAGC
Bpu1102I 550 GCTCAGC GC^T[ACGT]AGC
581 GCTCAGC
BsaHI 409 GACGCC G[GA]^CG[CT]C
622 GACGCC
BsmAI 4 GAGAC GTCTC(1/5)
597 GTCTC
BsrFI 440 ACCGGC [GA]^CCGG[CT]
1024 ACCGGC
BstYI 175 AGATCC [GA]^GATC[CT]
329 AGATCT
CelII 550 GCTCAGC GC^T[ACGT]AGC
581 GCTCAGC
Cfr10I 440 ACCGGC [GA]^CCGG[CT]
1024 ACCGGC
DpnII 176 GATC ^GATC
330 GATC
DsaI 359 CCACGG C^C[GA][CT]GG
944 CCATGG
FauI 156 GCGGG CCCGC(4/6)
214 GCGGG
HaeII 763 AGCGCC [GA]GCGC^[CT]
1000 AGCGCC
Hin1I 409 GACGCC G[GA]^CG[CT]C
622 GACGCC
MaeII 371 ACGT A^CGT
466 ACGT
MboI 176 GATC ^GATC
330 GATC
MboII 324 GAAGA GAAGA(8/7)
327 GAAGA
MflI 175 AGATCC [GA]^GATC[CT]
329 AGATCT
MspA1I 827 CAGCGG C[AC]G^C[GT]G
836 CAGCGG
NdeII 176 GATC ^GATC
330 GATC
SacI 250 GAGCTC GAGCT^C
952 GAGCTC
Sau3AI 176 GATC ^GATC
330 GATC
SfaNI 153 GATGC GCATC(5/9)
638 GCATC
XhoII 175 AGATCC [GA]^GATC[CT]
329 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
AvaII BanII BbvI BcnI BmyI
BsaJI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII EaeI EcoO109I EcoRII Fnu4HI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MnlI MslI MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PleI
Sau96I ScrFI TaqI TseI Tsp45I
Tsp509I TthHB8I