################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## Acc65I AccII AccIII AciI AclI AcsI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI EcoRV FauI FbaI FseI FspI HaeII HapII HgaI HhaI HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MfeI MluI MroI MslI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 610 GACGTC GACGT^C AccI 739 GTAGAC GT^[AC][GT]AC AcyI 610 GACGTC G[GA]^CG[CT]C AfaI 286 GTAC GT^AC AspHI 10 GAGCAC G[AT]GC[AT]^C AvaII 66 GGTCC G^G[AT]CC BalI 39 TGGCCA TGG^CCA BanI 128 GGCACC G^G[CT][GA]CC BbsI 664 GTCTTC GAAGAC(2/6) BglII 599 AGATCT A^GATCT BmyI 10 GAGCAC G[AGT]GC[ACT]^C BpuAI 664 GTCTTC GAAGAC(2/6) BsaAI 639 CACGTA [CT]AC^GT[GA] BsaBI 222 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 610 GACGTC G[GA]^CG[CT]C BsiHKAI 10 GAGCAC G[AT]GC[AT]^C Bsp1286I 10 GAGCAC G[AGT]GC[ACT]^C BssSI 670 CACGAG CACGAG(-5/-1) BstEII 150 GGTGACC G^GT[ACGT]ACC BstPI 150 GGTGACC G^GT[ACGT]ACC EaeI 39 TGGCCA [CT]^GGCC[GA] EcoNI 767 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 150 GGTGACC G^GT[ACGT]ACC Hin1I 610 GACGTC G[GA]^CG[CT]C HinfI 541 GAGTC G^A[ACGT]TC HphI 150 GGTGA GGTGA(8/7) MamI 222 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 39 TGGCCA TGG^CCA MscI 39 TGGCCA TGG^CCA MseI 395 TTAA T^TAA MspA1I 100 CAGCTG C[AC]G^C[GT]G NcoI 428 CCATGG C^CATGG PleI 541 GAGTC GAGTC(4/5) PvuII 100 CAGCTG CAG^CTG RsaI 286 GTAC GT^AC SfcI 534 CTACAG C^T[GA][CT]AG SmlI 745 CTCAAG C^T[CT][GA]AG StuI 775 AGGCCT AGG^CCT Tru9I 395 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AflIII 638 ACACGT A^C[GA][CT]GT 715 ACGTGT AlwI 227 GGATC GGATC(4/5) 626 GGATC BfaI 217 CTAG C^TAG 283 CTAG BsmFI 485 GGGAC GGGAC(10/14) 719 GTCCC BstYI 227 GGATCT [GA]^GATC[CT] 599 AGATCT EarI 451 CTCTTC CTCTTC(1/4) 619 GAAGAG EcoT22I 22 ATGCAT ATGCA^T 52 ATGCAT Ksp632I 451 CTCTTC CTCTTC(1/4) 619 GAAGAG MaeI 217 CTAG C^TAG 283 CTAG MflI 227 GGATCT [GA]^GATC[CT] 599 AGATCT NsiI 22 ATGCAT ATGCA^T 52 ATGCAT TaqI 8 TCGA T^CGA 813 TCGA Tsp45I 151 GTGAC ^GT[GC]AC 244 GTCAC TthHB8I 8 TCGA T^CGA 813 TCGA XhoII 227 GGATCT [GA]^GATC[CT] 599 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AluI BbvI BsaJI BsgI BsiYI BslI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DpnII DsaI EcoRII EcoT14I Fnu4HI FokI HaeIII ItaI MaeII MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV NspI Sau3AI Sau96I ScrFI SfaNI SphI StyI TseI TspRI