##################################
# NO CUT ENZYMES ( 184 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FokI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MaeII MamI
MfeI MflI MluI MroI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcsI 617 AAATTC [GA]^AATT[CT]
AfaI 538 GTAC GT^AC
AlwNI 557 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 617 AAATTC [GA]^AATT[CT]
AvaII 343 GGACC G^G[AT]CC
BalI 93 TGGCCA TGG^CCA
BanII 421 GAGCCC G[GA]GC[CT]^C
BbsI 77 GAAGAC GAAGAC(2/6)
BcnI 298 CCGGG CC^[GC]GG
BmyI 421 GAGCCC G[AGT]GC[ACT]^C
BpmI 72 CTCCAG CTGGAG(16/14)
BpuAI 77 GAAGAC GAAGAC(2/6)
BseRI 528 CTCCTC GAGGAG(10/8)
Bsp1286I 421 GAGCCC G[AGT]GC[ACT]^C
BsrI 53 CCAGT ACTGG(1/-1)
DpnII 522 GATC ^GATC
DraI 706 TTTAAA TTT^AAA
DraII 342 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 50 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 93 TGGCCA [CT]^GGCC[GA]
EcoO109I 342 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 589 CCCGC CCCGC(4/6)
HapII 298 CCGG C^CGG
HpaII 298 CCGG C^CGG
MaeIII 667 GTTAC ^GT[ACGT]AC
MboI 522 GATC ^GATC
MluNI 93 TGGCCA TGG^CCA
MscI 93 TGGCCA TGG^CCA
MspA1I 97 CAGCTG C[AC]G^C[GT]G
MspI 298 CCGG C^CGG
MwoI 583 GCAACCCCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 298 CCGGG CC^[GC]GG
NdeII 522 GATC ^GATC
NlaIII 41 CATG CATG^
NlaIV 132 GGTTCC GG[ACGT]^[ACGT]CC
PpuMI 342 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 97 CAGCTG CAG^CTG
RsaI 538 GTAC GT^AC
Sau3AI 522 GATC ^GATC
SfaNI 595 GCATC GCATC(5/9)
SfcI 117 CTACAG C^T[GA][CT]AG
SmlI 162 CTCAAG C^T[CT][GA]AG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AciI 443 CCGC CCGC(-3/-1)
590 CCGC
AluI 98 AGCT AG^CT
526 AGCT
BsaJI 198 CCCAGG C^C[ACGT][ACGT]GG
199 CCAGGG
BsmFI 143 GTCCC GGGAC(10/14)
196 GTCCC
BstNI 91 CCTGG CC^[AT]GG
199 CCAGG
Cac8I 95 GCCAGC GC[ACGT]^[ACGT]GC
561 GCCTGC
Cfr13I 21 GGGCC G^G[ACGT]CC
343 GGACC
DdeI 325 CTAAG C^T[ACGT]AG
419 CTGAG
EarI 185 CTCTTC CTCTTC(1/4)
546 CTCTTC
EcoRII 91 CCTGG ^CC[AT]GG
199 CCAGG
Ksp632I 185 CTCTTC CTCTTC(1/4)
546 CTCTTC
MvaI 91 CCTGG CC^[AT]GG
199 CCAGG
Sau96I 21 GGGCC G^G[ACGT]CC
343 GGACC
TaqI 228 TCGA T^CGA
288 TCGA
TfiI 68 GATTC G^A[AT]TC
271 GAATC
TthHB8I 228 TCGA T^CGA
288 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
BbvI BssKI Fnu4HI HaeIII HinfI
HphI ItaI MboII MnlI MseI
PleI ScrFI Tru9I TseI Tsp509I
TspRI