################################## # NO CUT ENZYMES ( 184 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MroI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcsI 617 AAATTC [GA]^AATT[CT] AfaI 538 GTAC GT^AC AlwNI 557 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 617 AAATTC [GA]^AATT[CT] AvaII 343 GGACC G^G[AT]CC BalI 93 TGGCCA TGG^CCA BanII 421 GAGCCC G[GA]GC[CT]^C BbsI 77 GAAGAC GAAGAC(2/6) BcnI 298 CCGGG CC^[GC]GG BmyI 421 GAGCCC G[AGT]GC[ACT]^C BpmI 72 CTCCAG CTGGAG(16/14) BpuAI 77 GAAGAC GAAGAC(2/6) BseRI 528 CTCCTC GAGGAG(10/8) Bsp1286I 421 GAGCCC G[AGT]GC[ACT]^C BsrI 53 CCAGT ACTGG(1/-1) DpnII 522 GATC ^GATC DraI 706 TTTAAA TTT^AAA DraII 342 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 50 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 93 TGGCCA [CT]^GGCC[GA] EcoO109I 342 AGGACCT [GA]G^G[ACGT]CC[CT] FauI 589 CCCGC CCCGC(4/6) HapII 298 CCGG C^CGG HpaII 298 CCGG C^CGG MaeIII 667 GTTAC ^GT[ACGT]AC MboI 522 GATC ^GATC MluNI 93 TGGCCA TGG^CCA MscI 93 TGGCCA TGG^CCA MspA1I 97 CAGCTG C[AC]G^C[GT]G MspI 298 CCGG C^CGG MwoI 583 GCAACCCCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 298 CCGGG CC^[GC]GG NdeII 522 GATC ^GATC NlaIII 41 CATG CATG^ NlaIV 132 GGTTCC GG[ACGT]^[ACGT]CC PpuMI 342 AGGACCT [GA]G^G[AT]CC[CT] PvuII 97 CAGCTG CAG^CTG RsaI 538 GTAC GT^AC Sau3AI 522 GATC ^GATC SfaNI 595 GCATC GCATC(5/9) SfcI 117 CTACAG C^T[GA][CT]AG SmlI 162 CTCAAG C^T[CT][GA]AG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AciI 443 CCGC CCGC(-3/-1) 590 CCGC AluI 98 AGCT AG^CT 526 AGCT BsaJI 198 CCCAGG C^C[ACGT][ACGT]GG 199 CCAGGG BsmFI 143 GTCCC GGGAC(10/14) 196 GTCCC BstNI 91 CCTGG CC^[AT]GG 199 CCAGG Cac8I 95 GCCAGC GC[ACGT]^[ACGT]GC 561 GCCTGC Cfr13I 21 GGGCC G^G[ACGT]CC 343 GGACC DdeI 325 CTAAG C^T[ACGT]AG 419 CTGAG EarI 185 CTCTTC CTCTTC(1/4) 546 CTCTTC EcoRII 91 CCTGG ^CC[AT]GG 199 CCAGG Ksp632I 185 CTCTTC CTCTTC(1/4) 546 CTCTTC MvaI 91 CCTGG CC^[AT]GG 199 CCAGG Sau96I 21 GGGCC G^G[ACGT]CC 343 GGACC TaqI 228 TCGA T^CGA 288 TCGA TfiI 68 GATTC G^A[AT]TC 271 GAATC TthHB8I 228 TCGA T^CGA 288 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### BbvI BssKI Fnu4HI HaeIII HinfI HphI ItaI MboII MnlI MseI PleI ScrFI Tru9I TseI Tsp509I TspRI