Restriction map for
pF1KB7374
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# NO CUT ENZYMES ( 178 enzymes) #
##################################
AatII Acc65I AccI AccIII AciI
AcyI AfaI AflIII AgeI AhdI
AluI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BglI BglII BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I Cac8I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MamI MfeI MflI
MluI MluNI MroI MscI MslI
MspA1I MspI MunI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PvuI
PvuII RsaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TaqI Tsp45I Tth111I
TthHB8I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 344 CGCG CG^CG
AclI 621 AACGTT AA^CGTT
AflII 502 CTTAAG C^TTAAG
AlwI 235 GATCC GGATC(4/5)
AlwNI 26 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 396 ATTAAT AT^TAAT
AsnI 396 ATTAAT AT^TAAT
AvrII 10 CCTAGG C^CTAGG
BfrI 502 CTTAAG C^TTAAG
BlnI 10 CCTAGG C^CTAGG
BsaJI 10 CCTAGG C^C[ACGT][ACGT]GG
BseRI 295 GAGGAG GAGGAG(10/8)
BsiYI 239 CCAGCATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 239 CCAGCATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 14 GGGAC GGGAC(10/14)
BspHI 411 TCATGA T^CATGA
BssKI 514 CCTGG ^CC[ACGT]GG
BstNI 514 CCTGG CC^[AT]GG
BstUI 344 CGCG CG^CG
CfoI 345 GCGC GCG^C
DdeI 383 CTCAG C^T[ACGT]AG
DraII 258 AGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 391 CGGCCA [CT]^GGCC[GA]
EcoO109I 258 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRII 514 CCTGG ^CC[AT]GG
EcoRV 472 GATATC GAT^ATC
EcoT14I 10 CCTAGG C^C[AT][AT]GG
HhaI 345 GCGC GCG^C
HinP1I 345 GCGC G^CGC
MaeII 622 ACGT A^CGT
MaeIII 663 GTTAC ^GT[ACGT]AC
MvaI 514 CCTGG CC^[AT]GG
MvnI 344 CGCG CG^CG
NlaIV 259 GGCCCC GG[ACGT]^[ACGT]CC
NspI 242 GCATGT [GA]CATG^[CT]
PflMI 239 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 55 GACTC GAGTC(4/5)
PshBI 396 ATTAAT AT^TAAT
Psp1406I 621 AACGTT AA^CGTT
PstI 302 CTGCAG CTGCA^G
RcaI 411 TCATGA T^CATGA
SapI 211 GAAGAGC GCTCTTC(1/4)
ScrFI 514 CCTGG CC^[ACGT]GG
SfcI 302 CTGCAG C^T[GA][CT]AG
SspI 399 AATATT AAT^ATT
StyI 10 CCTAGG C^C[AT][AT]GG
Van91I 239 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 12 enzymes) #
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BbsI 67 GAAGAC GAAGAC(2/6)
3 GTCTTC
BbvI 463 GCAGC GCAGC(8/12)
301 GCTGC
BpuAI 67 GAAGAC GAAGAC(2/6)
3 GTCTTC
BsmAI 85 GAGAC GTCTC(1/5)
272 GAGAC
BstF5I 201 GGATG GGATG(2/0)
291 GGATG
Fnu4HI 301 GCTGC GC^[ACGT]GC
463 GCAGC
FokI 201 GGATG GGATG(9/13)
291 GGATG
HphI 517 GGTGA GGTGA(8/7)
7 TCACC
ItaI 301 GCTGC GC^[ACGT]GC
463 GCAGC
SmlI 448 CTTGAG C^T[CT][GA]AG
502 CTTAAG
TseI 301 GCTGC G^C[AT]GC
463 GCAGC
TspRI 231 CAGTG CA[GC]TG[ACGT][ACGT]^
285 CAGTG
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AcsI ApoI BfaI Cfr13I DpnII
EarI HaeIII HinfI Ksp632I MaeI
MboI MboII MnlI MseI NdeII
NlaIII Sau3AI Sau96I TfiI Tru9I
Tsp509I