################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AcsI 518 AAATTC [GA]^AATT[CT] AhdI 590 GACTACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 467 GGATC GGATC(4/5) ApoI 518 AAATTC [GA]^AATT[CT] AspEI 590 GACTACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanII 684 GGGCTC G[GA]GC[CT]^C BbsI 424 GTCTTC GAAGAC(2/6) BpuAI 424 GTCTTC GAAGAC(2/6) Bsp1407I 773 TGTACA T^GTACA BsrGI 773 TGTACA T^GTACA Cac8I 477 GCTTGC GC[ACGT]^[ACGT]GC DrdI 447 GACCTTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 131 CCATGG C^C[GA][CT]GG Eam1105I 590 GACTACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 386 CTCTTC CTCTTC(1/4) HincII 191 GTCAAC GT[CT]^[GA]AC HindII 191 GTCAAC GT[CT]^[GA]AC Ksp632I 386 CTCTTC CTCTTC(1/4) MaeIII 380 GTCAC ^GT[ACGT]AC MseI 928 TTAA T^TAA MspA1I 482 CAGCTG C[AC]G^C[GT]G MwoI 346 GCTGTCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 131 CCATGG C^CATGG PleI 746 GACTC GAGTC(4/5) PstI 504 CTGCAG CTGCA^G PvuII 482 CAGCTG CAG^CTG SfaNI 98 GCATC GCATC(5/9) SspBI 773 TGTACA T^GTACA SspI 124 AATATT AAT^ATT StuI 707 AGGCCT AGG^CCT TaqI 666 TCGA T^CGA Tru9I 928 TTAA T^TAA Tsp45I 380 GTCAC ^GT[GC]AC Tsp509I 519 AATT ^AATT TspRI 107 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 666 TCGA T^CGA XcmI 678 CCACATGGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) 876 CCGC BmyI 684 GGGCTC G[AGT]GC[ACT]^C 896 GGGCAC BsaI 780 GAGACC GGTCTC(1/5) 455 GGTCTC BsgI 222 CTGCAC GTGCAG(16/14) 564 CTGCAC BsiYI 363 CCGCTATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 732 CCTGTCTGTGG BslI 363 CCGCTATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 732 CCTGTCTGTGG BsmAI 780 GAGAC GTCTC(1/5) 456 GTCTC Bsp1286I 684 GGGCTC G[AGT]GC[ACT]^C 896 GGGCAC BspHI 404 TCATGA T^CATGA 428 TCATGA BspMI 718 ACCTGC ACCTGC(4/8) 283 GCAGGT BsrI 108 ACTGG ACTGG(1/-1) 628 CCAGT DdeI 187 CTCAG C^T[ACGT]AG 296 CTCAG DpnII 243 GATC ^GATC 468 GATC EcoT14I 131 CCATGG C^C[AT][AT]GG 449 CCTTGG HaeIII 708 GGCC GG^CC 825 GGCC HphI 381 TCACC GGTGA(8/7) 842 TCACC MboI 243 GATC ^GATC 468 GATC NdeII 243 GATC ^GATC 468 GATC RcaI 404 TCATGA T^CATGA 428 TCATGA Sau3AI 243 GATC ^GATC 468 GATC SfcI 504 CTGCAG C^T[GA][CT]AG 901 CTACAG StyI 131 CCATGG C^C[AT][AT]GG 449 CCTTGG TfiI 47 GAATC G^A[AT]TC 408 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AfaI AluI AvaII BbvI BsaJI BseRI BsmFI BssKI BstF5I BstNI Cfr13I EcoRII Fnu4HI FokI HinfI ItaI MboII MnlI MslI MvaI NlaIII RsaI Sau96I ScrFI TseI