Restriction map for pF1KB7375
##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
BspDI       BspEI       BspLU11I    BsrBI       BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      EaeI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MaeI        
MaeII       MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SwaI        
Tth111I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   37 enzymes) #
###################################

AcsI          518  AAATTC               [GA]^AATT[CT]
AhdI          590  GACTACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          467  GGATC                GGATC(4/5)
ApoI          518  AAATTC               [GA]^AATT[CT]
AspEI         590  GACTACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII         684  GGGCTC               G[GA]GC[CT]^C
BbsI          424  GTCTTC               GAAGAC(2/6)
BpuAI         424  GTCTTC               GAAGAC(2/6)
Bsp1407I      773  TGTACA               T^GTACA
BsrGI         773  TGTACA               T^GTACA
Cac8I         477  GCTTGC               GC[ACGT]^[ACGT]GC
DrdI          447  GACCTTGGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          131  CCATGG               C^C[GA][CT]GG
Eam1105I      590  GACTACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          386  CTCTTC               CTCTTC(1/4)
HincII        191  GTCAAC               GT[CT]^[GA]AC
HindII        191  GTCAAC               GT[CT]^[GA]AC
Ksp632I       386  CTCTTC               CTCTTC(1/4)
MaeIII        380  GTCAC                ^GT[ACGT]AC
MseI          928  TTAA                 T^TAA
MspA1I        482  CAGCTG               C[AC]G^C[GT]G
MwoI          346  GCTGTCATGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          131  CCATGG               C^CATGG
PleI          746  GACTC                GAGTC(4/5)
PstI          504  CTGCAG               CTGCA^G
PvuII         482  CAGCTG               CAG^CTG
SfaNI          98  GCATC                GCATC(5/9)
SspBI         773  TGTACA               T^GTACA
SspI          124  AATATT               AAT^ATT
StuI          707  AGGCCT               AGG^CCT
TaqI          666  TCGA                 T^CGA
Tru9I         928  TTAA                 T^TAA
Tsp45I        380  GTCAC                ^GT[GC]AC
Tsp509I       519  AATT                 ^AATT
TspRI         107  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       666  TCGA                 T^CGA
XcmI          678  CCACATGGGCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AciI          363  CCGC                 CCGC(-3/-1)
              876  CCGC                
BmyI          684  GGGCTC               G[AGT]GC[ACT]^C
              896  GGGCAC              
BsaI          780  GAGACC               GGTCTC(1/5)
              455  GGTCTC              
BsgI          222  CTGCAC               GTGCAG(16/14)
              564  CTGCAC              
BsiYI         363  CCGCTATGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              732  CCTGTCTGTGG         
BslI          363  CCGCTATGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              732  CCTGTCTGTGG         
BsmAI         780  GAGAC                GTCTC(1/5)
              456  GTCTC               
Bsp1286I      684  GGGCTC               G[AGT]GC[ACT]^C
              896  GGGCAC              
BspHI         404  TCATGA               T^CATGA
              428  TCATGA              
BspMI         718  ACCTGC               ACCTGC(4/8)
              283  GCAGGT              
BsrI          108  ACTGG                ACTGG(1/-1)
              628  CCAGT               
DdeI          187  CTCAG                C^T[ACGT]AG
              296  CTCAG               
DpnII         243  GATC                 ^GATC
              468  GATC                
EcoT14I       131  CCATGG               C^C[AT][AT]GG
              449  CCTTGG              
HaeIII        708  GGCC                 GG^CC
              825  GGCC                
HphI          381  TCACC                GGTGA(8/7)
              842  TCACC               
MboI          243  GATC                 ^GATC
              468  GATC                
NdeII         243  GATC                 ^GATC
              468  GATC                
RcaI          404  TCATGA               T^CATGA
              428  TCATGA              
Sau3AI        243  GATC                 ^GATC
              468  GATC                
SfcI          504  CTGCAG               C^T[GA][CT]AG
              901  CTACAG              
StyI          131  CCATGG               C^C[AT][AT]GG
              449  CCTTGG              
TfiI           47  GAATC                G^A[AT]TC
              408  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   25 enzymes) #
#########################################

AfaI        AluI        AvaII       BbvI        BsaJI       
BseRI       BsmFI       BssKI       BstF5I      BstNI       
Cfr13I      EcoRII      Fnu4HI      FokI        HinfI       
ItaI        MboII       MnlI        MslI        MvaI        
NlaIII      RsaI        Sau96I      ScrFI       TseI        
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