Restriction map for
pF1KB7378
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AviII AvrII BalI BbsI
BbvCI BcgI BciVI BfrI BlnI
BlpI Bpu1102I BpuAI BsaI BsaWI
BseAI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssSI Bst1107I
BstBI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco47III EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MboII MfeI
MluI MluNI MroI MscI MseI
MslI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfuI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AlwNI 369 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 583 CCTGAGG CC^T[ACGT]AGG
ApaI 651 GGGCCC GGGCC^C
AspI 619 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 80 GGATCC G^GATCC
BbrPI 558 CACGTG CAC^GTG
BclI 686 TGATCA T^GATCA
BfaI 781 CTAG C^TAG
BglII 783 AGATCT A^GATCT
BpmI 601 CTGGAG CTGGAG(16/14)
BsaAI 558 CACGTG [CT]AC^GT[GA]
BsaBI 681 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 604 GAGGAG GAGGAG(10/8)
BsgI 427 CTGCAC GTGCAG(16/14)
BsmAI 12 GTCTC GTCTC(1/5)
BstAPI 335 GCACCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 421 GGTCACC G^GT[ACGT]ACC
BstF5I 708 GGATG GGATG(2/0)
BstPI 421 GGTCACC G^GT[ACGT]ACC
Bsu36I 583 CCTGAGG CC^T[ACGT]AGG
DsaI 671 CCACGG C^C[GA][CT]GG
EagI 96 CGGCCG C^GGCCG
EclXI 96 CGGCCG C^GGCCG
Eco52I 96 CGGCCG C^GGCCG
Eco81I 583 CCTGAGG CC^T[ACGT]AGG
EcoO65I 421 GGTCACC G^GT[ACGT]ACC
EcoT14I 75 CCTTGG C^C[AT][AT]GG
FbaI 686 TGATCA T^GATCA
FokI 708 GGATG GGATG(9/13)
HgaI 371 GCGTC GACGC(5/10)
HinfI 757 GAGTC G^A[ACGT]TC
MaeI 781 CTAG C^TAG
MamI 681 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflFI 619 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 757 GAGTC GAGTC(4/5)
PmaCI 558 CACGTG CAC^GTG
PmlI 558 CACGTG CAC^GTG
SgrAI 240 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 163 CCCGGG CCC^GGG
StyI 75 CCTTGG C^C[AT][AT]GG
TaqI 179 TCGA T^CGA
Tth111I 619 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 179 TCGA T^CGA
XbaI 780 TCTAGA T^CTAGA
XcmI 550 CCAGTCATCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 163 CCCGGG C^CCGGG
XmaI 163 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AfaI 363 GTAC GT^AC
720 GTAC
AvaII 115 GGTCC G^G[AT]CC
618 GGACC
BanII 310 GGGCTC G[GA]GC[CT]^C
651 GGGCCC
BglI 236 GCCGCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
283 GCCGCTGGGGC
BsiEI 96 CGGCCG CG[GA][CT]^CG
354 CGGTCG
BspMI 425 ACCTGC ACCTGC(4/8)
510 GCAGGT
BstXI 279 CCAGGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
737 CCAACATGGTGG
DraII 650 GGGGCCC [GA]G^G[ACGT]CC[CT]
651 GGGCCCT
EaeI 96 CGGCCG [CT]^GGCC[GA]
124 CGGCCA
EcoNI 426 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
696 CCTGCGAAAGG
EcoO109I 650 GGGGCCC [GA]G^G[ACGT]CC[CT]
651 GGGCCCT
HphI 423 TCACC GGTGA(8/7)
500 TCACC
PflMI 594 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
671 CCACGGCCTGG
RsaI 363 GTAC GT^AC
720 GTAC
Van91I 594 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
671 CCACGGCCTGG
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AvaI BanI BbeI BbvI BcnI
BmyI BsaHI BsaJI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssHII BssKI BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII ItaI KasI MaeII MaeIII
MboI MflI MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
Sau3AI Sau96I ScrFI SfoI TseI
Tsp45I TspRI XhoII