##################################
# NO CUT ENZYMES (  205 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        Fnu4HI      FokI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       HphI        ItaI        KasI        
KpnI        KspI        MaeII       MaeIII      MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MspI        MunI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StyI        
SwaI        TaqI        Tru9I       TseI        Tsp45I      
TspRI       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   20 enzymes) #
###################################

AcsI          189  AAATTC               [GA]^AATT[CT]
ApoI          189  AAATTC               [GA]^AATT[CT]
AvaI          218  CCCGAG               C^[CT]CG[GA]G
BanI           87  GGCACC               G^G[CT][GA]CC
BanII          42  GAGCCC               G[GA]GC[CT]^C
BmyI           42  GAGCCC               G[AGT]GC[ACT]^C
BpmI          202  CTGGAG               CTGGAG(16/14)
BsmFI         216  GTCCC                GGGAC(10/14)
BsoBI         218  CCCGAG               C^[CT]CG[GA]G
Bsp1286I       42  GAGCCC               G[AGT]GC[ACT]^C
Cac8I         119  GCTTGC               GC[ACGT]^[ACGT]GC
HaeIII        262  GGCC                 GG^CC
MspA1I        272  CAGCTG               C[AC]G^C[GT]G
PvuII         272  CAGCTG               CAG^CTG
SapI           39  GAAGAGC              GCTCTTC(1/4)
SfaNI          78  GATGC                GCATC(5/9)
SfcI          168  CTACAG               C^T[GA][CT]AG
StuI          261  AGGCCT               AGG^CCT
TfiI           52  GAATC                G^A[AT]TC
Tsp509I       190  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AluI          118  AGCT                 AG^CT
              273  AGCT                
AvaII         210  GGACC                G^G[AT]CC
              462  GGACC               
BfaI          265  CTAG                 C^TAG
              499  CTAG                
BseRI         247  GAGGAG               GAGGAG(10/8)
              250  GAGGAG              
BsiYI         243  CCAAGAGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              466  CCACCTGAAGG         
BslI          243  CCAAGAGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              466  CCACCTGAAGG         
BsrI          201  ACTGG                ACTGG(1/-1)
              213  CCAGT               
Cfr13I        210  GGACC                G^G[ACGT]CC
              462  GGACC               
DdeI          102  CTTAG                C^T[ACGT]AG
              270  CTCAG               
EarI           33  GAAGAG               CTCTTC(1/4)
               39  GAAGAG              
Ksp632I        33  GAAGAG               CTCTTC(1/4)
               39  GAAGAG              
MaeI          265  CTAG                 C^TAG
              499  CTAG                
NlaIV          87  GGCACC               GG[ACGT]^[ACGT]CC
              462  GGACCC              
Sau96I        210  GGACC                G^G[ACGT]CC
              462  GGACC               


#########################################
# 3 OR MORE CUT ENZYMES (   19 enzymes) #
#########################################

AlwI        BbsI        BpuAI       BssKI       BstNI       
BstYI       DpnII       EcoRII      HinfI       MboI        
MboII       MflI        MnlI        MvaI        NdeII       
PleI        Sau3AI      ScrFI       XhoII