Restriction map for
pF1KB7381
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BalI BbrPI
BbvCI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI Eam1105I EarI Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HindIII HpaI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MslI
MunI NaeI NcoI NdeI NgoMIV
NheI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SapI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI StuI SwaI
TfiI Van91I XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 410 GTCGAC GT^[AC][GT]AC
AcyI 392 GGCGCC G[GA]^CG[CT]C
AfaI 231 GTAC GT^AC
AflIII 227 ACATGT A^C[GA][CT]GT
AlwNI 334 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 828 CCTGAGG CC^T[ACGT]AGG
AspI 289 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 969 GGATCC G^GATCC
BbeI 392 GGCGCC GGCGC^C
BbsI 813 GAAGAC GAAGAC(2/6)
BfaI 1093 CTAG C^TAG
BglII 494 AGATCT A^GATCT
BpuAI 813 GAAGAC GAAGAC(2/6)
BsaHI 392 GGCGCC G[GA]^CG[CT]C
BsmI 945 GAATGC GAATGC(1/-1)
BspLU11I 227 ACATGT A^CATGT
BsrDI 602 GCAATG GCAATG(2/0)
BstAPI 429 GCAGGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 828 CCTGAGG CC^T[ACGT]AGG
Eco81I 828 CCTGAGG CC^T[ACGT]AGG
Hin1I 392 GGCGCC G[GA]^CG[CT]C
HincII 410 GTCGAC GT[CT]^[GA]AC
HindII 410 GTCGAC GT[CT]^[GA]AC
KasI 392 GGCGCC G^GCGCC
MaeI 1093 CTAG C^TAG
MaeII 293 ACGT A^CGT
MaeIII 514 GTGAC ^GT[ACGT]AC
MboII 813 GAAGA GAAGA(8/7)
NarI 392 GGCGCC GG^CGCC
NotI 323 GCGGCCGC GC^GGCCGC
NspI 227 ACATGT [GA]CATG^[CT]
PflFI 289 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 403 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 14 CAGCTG CAG^CTG
RsaI 231 GTAC GT^AC
SalI 410 GTCGAC G^TCGAC
SbfI 1081 CCTGCAGG CCTGCA^GG
SexAI 690 ACCTGGT A^CC[AT]GGT
SfoI 392 GGCGCC GGC^GCC
SmlI 1064 CTCAAG C^T[CT][GA]AG
Sse8387I 1081 CCTGCAGG CCTGCA^GG
Tsp45I 514 GTGAC ^GT[GC]AC
Tth111I 289 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1092 TCTAGA T^CTAGA
XcmI 567 CCAGTGTCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 758 AAATTT [GA]^AATT[CT]
1218 AAATTC
ApoI 758 AAATTT [GA]^AATT[CT]
1218 AAATTC
BcgI 206 CGAGCTTCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
311 CGAGCTTCGTGC
BsiEI 324 CGGCCG CG[GA][CT]^CG
384 CGGCCG
BsrBI 123 GAGCGG CCGCTC(-3/-3)
1183 GAGCGG
BstYI 494 AGATCT [GA]^GATC[CT]
969 GGATCC
DsaI 26 CCACGG C^C[GA][CT]GG
531 CCACGG
EaeI 324 CGGCCG [CT]^GGCC[GA]
384 CGGCCG
EagI 324 CGGCCG C^GGCCG
384 CGGCCG
EclXI 324 CGGCCG C^GGCCG
384 CGGCCG
Eco52I 324 CGGCCG C^GGCCG
384 CGGCCG
EcoNI 699 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
958 CCTGAACCAGG
HinfI 816 GACTC G^A[ACGT]TC
1032 GAGTC
MflI 494 AGATCT [GA]^GATC[CT]
969 GGATCC
MseI 1216 TTAA T^TAA
1318 TTAA
PleI 816 GACTC GAGTC(4/5)
1032 GAGTC
PstI 703 CTGCAG CTGCA^G
1082 CTGCAG
SacI 475 GAGCTC GAGCT^C
648 GAGCTC
SfcI 703 CTGCAG C^T[GA][CT]AG
1082 CTGCAG
Tru9I 1216 TTAA T^TAA
1318 TTAA
Tsp509I 759 AATT ^AATT
1219 AATT
XhoII 494 AGATCT [GA]^GATC[CT]
969 GGATCC
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AluI AlwI ApaI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MboI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PpuMI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI TspRI TthHB8I